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The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays
Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumptio...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700135/ https://www.ncbi.nlm.nih.gov/pubmed/34943947 http://dx.doi.org/10.3390/cells10123439 |
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author | Frattolin, Jennifer Watson, Daniel J. Bonneuil, Willy V. Russell, Matthew J. Fasanella Masci, Francesca Bandara, Mikaila Brook, Bindi S. Nibbs, Robert J. B. Moore, James E. |
author_facet | Frattolin, Jennifer Watson, Daniel J. Bonneuil, Willy V. Russell, Matthew J. Fasanella Masci, Francesca Bandara, Mikaila Brook, Bindi S. Nibbs, Robert J. B. Moore, James E. |
author_sort | Frattolin, Jennifer |
collection | PubMed |
description | Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. |
format | Online Article Text |
id | pubmed-8700135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87001352021-12-24 The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays Frattolin, Jennifer Watson, Daniel J. Bonneuil, Willy V. Russell, Matthew J. Fasanella Masci, Francesca Bandara, Mikaila Brook, Bindi S. Nibbs, Robert J. B. Moore, James E. Cells Review Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. MDPI 2021-12-07 /pmc/articles/PMC8700135/ /pubmed/34943947 http://dx.doi.org/10.3390/cells10123439 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Frattolin, Jennifer Watson, Daniel J. Bonneuil, Willy V. Russell, Matthew J. Fasanella Masci, Francesca Bandara, Mikaila Brook, Bindi S. Nibbs, Robert J. B. Moore, James E. The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays |
title | The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays |
title_full | The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays |
title_fullStr | The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays |
title_full_unstemmed | The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays |
title_short | The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays |
title_sort | critical importance of spatial and temporal scales in designing and interpreting immune cell migration assays |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700135/ https://www.ncbi.nlm.nih.gov/pubmed/34943947 http://dx.doi.org/10.3390/cells10123439 |
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