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The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays

Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumptio...

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Autores principales: Frattolin, Jennifer, Watson, Daniel J., Bonneuil, Willy V., Russell, Matthew J., Fasanella Masci, Francesca, Bandara, Mikaila, Brook, Bindi S., Nibbs, Robert J. B., Moore, James E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700135/
https://www.ncbi.nlm.nih.gov/pubmed/34943947
http://dx.doi.org/10.3390/cells10123439
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author Frattolin, Jennifer
Watson, Daniel J.
Bonneuil, Willy V.
Russell, Matthew J.
Fasanella Masci, Francesca
Bandara, Mikaila
Brook, Bindi S.
Nibbs, Robert J. B.
Moore, James E.
author_facet Frattolin, Jennifer
Watson, Daniel J.
Bonneuil, Willy V.
Russell, Matthew J.
Fasanella Masci, Francesca
Bandara, Mikaila
Brook, Bindi S.
Nibbs, Robert J. B.
Moore, James E.
author_sort Frattolin, Jennifer
collection PubMed
description Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response.
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spelling pubmed-87001352021-12-24 The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays Frattolin, Jennifer Watson, Daniel J. Bonneuil, Willy V. Russell, Matthew J. Fasanella Masci, Francesca Bandara, Mikaila Brook, Bindi S. Nibbs, Robert J. B. Moore, James E. Cells Review Intravital microscopy and other direct-imaging techniques have allowed for a characterisation of leukocyte migration that has revolutionised the field of immunology, resulting in an unprecedented understanding of the mechanisms of immune response and adaptive immunity. However, there is an assumption within the field that modern imaging techniques permit imaging parameters where the resulting cell track accurately captures a cell’s motion. This notion is almost entirely untested, and the relationship between what could be observed at a given scale and the underlying cell behaviour is undefined. Insufficient spatial and temporal resolutions within migration assays can result in misrepresentation of important physiologic processes or cause subtle changes in critical cell behaviour to be missed. In this review, we contextualise how scale can affect the perceived migratory behaviour of cells, summarise the limited approaches to mitigate this effect, and establish the need for a widely implemented framework to account for scale and correct observations of cell motion. We then extend the concept of scale to new approaches that seek to bridge the current “black box” between single-cell behaviour and systemic response. MDPI 2021-12-07 /pmc/articles/PMC8700135/ /pubmed/34943947 http://dx.doi.org/10.3390/cells10123439 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Frattolin, Jennifer
Watson, Daniel J.
Bonneuil, Willy V.
Russell, Matthew J.
Fasanella Masci, Francesca
Bandara, Mikaila
Brook, Bindi S.
Nibbs, Robert J. B.
Moore, James E.
The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays
title The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays
title_full The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays
title_fullStr The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays
title_full_unstemmed The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays
title_short The Critical Importance of Spatial and Temporal Scales in Designing and Interpreting Immune Cell Migration Assays
title_sort critical importance of spatial and temporal scales in designing and interpreting immune cell migration assays
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700135/
https://www.ncbi.nlm.nih.gov/pubmed/34943947
http://dx.doi.org/10.3390/cells10123439
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