Cargando…

The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?

Mucin (MUC) 8 has been shown to play an important role in respiratory disease and inflammatory responses. In the present study, we investigated the question of whether MUC8 is also produced and secreted by salivary glands and whether it may also play a role in the oral cavity in the context of infla...

Descripción completa

Detalles Bibliográficos
Autores principales: Schicht, Martin, Reichle, Adrian, Schapher, Mirco, Garreis, Fabian, Kleinsasser, Benedikt, Aydin, Malik, Sahin, Afsun, Iro, Heinrich, Paulsen, Friedrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700234/
https://www.ncbi.nlm.nih.gov/pubmed/34943565
http://dx.doi.org/10.3390/diagnostics11122330
_version_ 1784620707857039360
author Schicht, Martin
Reichle, Adrian
Schapher, Mirco
Garreis, Fabian
Kleinsasser, Benedikt
Aydin, Malik
Sahin, Afsun
Iro, Heinrich
Paulsen, Friedrich
author_facet Schicht, Martin
Reichle, Adrian
Schapher, Mirco
Garreis, Fabian
Kleinsasser, Benedikt
Aydin, Malik
Sahin, Afsun
Iro, Heinrich
Paulsen, Friedrich
author_sort Schicht, Martin
collection PubMed
description Mucin (MUC) 8 has been shown to play an important role in respiratory disease and inflammatory responses. In the present study, we investigated the question of whether MUC8 is also produced and secreted by salivary glands and whether it may also play a role in the oral cavity in the context of inflammatory processes or in the context of salivary stone formation. Tissue samples from parotid and submandibular glands of body donors (n = 6, age range 63–88 years), as well as surgically removed salivary stones from patients (n = 38, age range 48–72 years) with parotid and submandibular stone disease were immunohistochemically analyzed targeting MUC8 and TNFα. The presence of MUC8 in salivary stones was additionally analyzed by dot blot analyses. Moreover, saliva samples from patients (n = 10, age range 51–72 years), who had a salivary stone of the submandibular gland on one side were compared with saliva samples from the other “healthy” side, which did not have a salivary stone, by ELISA. Positive MUC8 was detectable in the inter- and intralobular excretory ducts of both glands (parotid and submandibular). The glandular acini showed no reactivity. TNFα revealed comparable reactivity to MUC8 in the glandular excretory ducts and also did not react in glandular acini. Salivary stones demonstrated a characteristic distribution pattern of MUC8 that differed between parotid and submandibular salivary stones. The mean MUC8 concentration was 71.06 ng/mL in female and 33.21 ng/mL in male subjects (p = 0.156). Saliva from the side with salivary calculi contained significantly (15-fold) higher MUC8 concentration levels than saliva from the healthy side (p = 0.0005). MUC8 concentration in salivary stones varied from 4.59 ng/mL to 202.83 ng/mL. In females, the MUC8 concentration in salivary stones was significantly (2.3-fold) higher, with an average of 82.84 ng/mL compared to 25.27 ng/mL in male patients (p = 0.034). MUC8 is secreted in the excretory duct system of salivary glands and released into saliva. Importantly, MUC8 salivary concentrations vary greatly between individuals. In addition, the MUC8 concentration is gender-dependent (♀ > ♂). In the context of salivary stone diseases, MUC8 is highly secreted in saliva. The findings support a role for MUC8 in the context of inflammatory events and salivary stone formation. The findings allow conclusions on a gender-dependent component of MUC8.
format Online
Article
Text
id pubmed-8700234
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87002342021-12-24 The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease? Schicht, Martin Reichle, Adrian Schapher, Mirco Garreis, Fabian Kleinsasser, Benedikt Aydin, Malik Sahin, Afsun Iro, Heinrich Paulsen, Friedrich Diagnostics (Basel) Article Mucin (MUC) 8 has been shown to play an important role in respiratory disease and inflammatory responses. In the present study, we investigated the question of whether MUC8 is also produced and secreted by salivary glands and whether it may also play a role in the oral cavity in the context of inflammatory processes or in the context of salivary stone formation. Tissue samples from parotid and submandibular glands of body donors (n = 6, age range 63–88 years), as well as surgically removed salivary stones from patients (n = 38, age range 48–72 years) with parotid and submandibular stone disease were immunohistochemically analyzed targeting MUC8 and TNFα. The presence of MUC8 in salivary stones was additionally analyzed by dot blot analyses. Moreover, saliva samples from patients (n = 10, age range 51–72 years), who had a salivary stone of the submandibular gland on one side were compared with saliva samples from the other “healthy” side, which did not have a salivary stone, by ELISA. Positive MUC8 was detectable in the inter- and intralobular excretory ducts of both glands (parotid and submandibular). The glandular acini showed no reactivity. TNFα revealed comparable reactivity to MUC8 in the glandular excretory ducts and also did not react in glandular acini. Salivary stones demonstrated a characteristic distribution pattern of MUC8 that differed between parotid and submandibular salivary stones. The mean MUC8 concentration was 71.06 ng/mL in female and 33.21 ng/mL in male subjects (p = 0.156). Saliva from the side with salivary calculi contained significantly (15-fold) higher MUC8 concentration levels than saliva from the healthy side (p = 0.0005). MUC8 concentration in salivary stones varied from 4.59 ng/mL to 202.83 ng/mL. In females, the MUC8 concentration in salivary stones was significantly (2.3-fold) higher, with an average of 82.84 ng/mL compared to 25.27 ng/mL in male patients (p = 0.034). MUC8 is secreted in the excretory duct system of salivary glands and released into saliva. Importantly, MUC8 salivary concentrations vary greatly between individuals. In addition, the MUC8 concentration is gender-dependent (♀ > ♂). In the context of salivary stone diseases, MUC8 is highly secreted in saliva. The findings support a role for MUC8 in the context of inflammatory events and salivary stone formation. The findings allow conclusions on a gender-dependent component of MUC8. MDPI 2021-12-10 /pmc/articles/PMC8700234/ /pubmed/34943565 http://dx.doi.org/10.3390/diagnostics11122330 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schicht, Martin
Reichle, Adrian
Schapher, Mirco
Garreis, Fabian
Kleinsasser, Benedikt
Aydin, Malik
Sahin, Afsun
Iro, Heinrich
Paulsen, Friedrich
The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?
title The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?
title_full The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?
title_fullStr The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?
title_full_unstemmed The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?
title_short The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?
title_sort translational role of muc8 in salivary glands: a potential biomarker for salivary stone disease?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700234/
https://www.ncbi.nlm.nih.gov/pubmed/34943565
http://dx.doi.org/10.3390/diagnostics11122330
work_keys_str_mv AT schichtmartin thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT reichleadrian thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT schaphermirco thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT garreisfabian thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT kleinsasserbenedikt thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT aydinmalik thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT sahinafsun thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT iroheinrich thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT paulsenfriedrich thetranslationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT schichtmartin translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT reichleadrian translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT schaphermirco translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT garreisfabian translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT kleinsasserbenedikt translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT aydinmalik translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT sahinafsun translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT iroheinrich translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease
AT paulsenfriedrich translationalroleofmuc8insalivaryglandsapotentialbiomarkerforsalivarystonedisease