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Genomic Evaluation of the Genus Coltivirus Indicates Genetic Diversity among Colorado Tick Fever Virus Strains and Demarcation of a New Species

The type species of the genus Coltivirus, Colorado tick fever virus (CTFV), was discovered in 1943 and is the most common tick-borne viral infection in the Western US. Despite its long history, very little is known about the molecular diversity of viruses classified within the species Colorado tick...

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Autores principales: Hughes, Holly R., Velez, Jason O., Fitzpatrick, Kelly, Davis, Emily H., Russell, Brandy J., Lambert, Amy J., Staples, J. Erin, Brault, Aaron C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700517/
https://www.ncbi.nlm.nih.gov/pubmed/34940030
http://dx.doi.org/10.3390/diseases9040092
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author Hughes, Holly R.
Velez, Jason O.
Fitzpatrick, Kelly
Davis, Emily H.
Russell, Brandy J.
Lambert, Amy J.
Staples, J. Erin
Brault, Aaron C.
author_facet Hughes, Holly R.
Velez, Jason O.
Fitzpatrick, Kelly
Davis, Emily H.
Russell, Brandy J.
Lambert, Amy J.
Staples, J. Erin
Brault, Aaron C.
author_sort Hughes, Holly R.
collection PubMed
description The type species of the genus Coltivirus, Colorado tick fever virus (CTFV), was discovered in 1943 and is the most common tick-borne viral infection in the Western US. Despite its long history, very little is known about the molecular diversity of viruses classified within the species Colorado tick fever coltivirus. Previous studies have suggested genetic variants and potential serotypes of CTFV, but limited genetic sequence information is available for CTFV strains. To address this knowledge gap, we report herein the full-length genomes of five strains of CTFV, including Salmon River virus and California hare coltivirus (CTFV-Ca). The sequence from the full-length genome of Salmon River virus identified a high genetic identity to the CTFV prototype strain with >90% amino acid identity in all the segments except segment four, suggesting Salmon River virus is a strain of the species Colorado tick fever coltivirus. Additionally, analysis suggests that segment four has been associated with reassortment in at least one strain. The CTFV-Ca full-length genomic sequence was highly variable from the prototype CTFV in all the segments. The genome of CTFV-Ca was most similar to the Eyach virus, including similar segments six and seven. These data suggest that CTFV-Ca is not a strain of CTFV but a unique species. Additional sequence information of CTFV strains will improve the molecular surveillance tools and provide additional taxonomic resolution to this understudied virus.
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spelling pubmed-87005172021-12-24 Genomic Evaluation of the Genus Coltivirus Indicates Genetic Diversity among Colorado Tick Fever Virus Strains and Demarcation of a New Species Hughes, Holly R. Velez, Jason O. Fitzpatrick, Kelly Davis, Emily H. Russell, Brandy J. Lambert, Amy J. Staples, J. Erin Brault, Aaron C. Diseases Article The type species of the genus Coltivirus, Colorado tick fever virus (CTFV), was discovered in 1943 and is the most common tick-borne viral infection in the Western US. Despite its long history, very little is known about the molecular diversity of viruses classified within the species Colorado tick fever coltivirus. Previous studies have suggested genetic variants and potential serotypes of CTFV, but limited genetic sequence information is available for CTFV strains. To address this knowledge gap, we report herein the full-length genomes of five strains of CTFV, including Salmon River virus and California hare coltivirus (CTFV-Ca). The sequence from the full-length genome of Salmon River virus identified a high genetic identity to the CTFV prototype strain with >90% amino acid identity in all the segments except segment four, suggesting Salmon River virus is a strain of the species Colorado tick fever coltivirus. Additionally, analysis suggests that segment four has been associated with reassortment in at least one strain. The CTFV-Ca full-length genomic sequence was highly variable from the prototype CTFV in all the segments. The genome of CTFV-Ca was most similar to the Eyach virus, including similar segments six and seven. These data suggest that CTFV-Ca is not a strain of CTFV but a unique species. Additional sequence information of CTFV strains will improve the molecular surveillance tools and provide additional taxonomic resolution to this understudied virus. MDPI 2021-12-17 /pmc/articles/PMC8700517/ /pubmed/34940030 http://dx.doi.org/10.3390/diseases9040092 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hughes, Holly R.
Velez, Jason O.
Fitzpatrick, Kelly
Davis, Emily H.
Russell, Brandy J.
Lambert, Amy J.
Staples, J. Erin
Brault, Aaron C.
Genomic Evaluation of the Genus Coltivirus Indicates Genetic Diversity among Colorado Tick Fever Virus Strains and Demarcation of a New Species
title Genomic Evaluation of the Genus Coltivirus Indicates Genetic Diversity among Colorado Tick Fever Virus Strains and Demarcation of a New Species
title_full Genomic Evaluation of the Genus Coltivirus Indicates Genetic Diversity among Colorado Tick Fever Virus Strains and Demarcation of a New Species
title_fullStr Genomic Evaluation of the Genus Coltivirus Indicates Genetic Diversity among Colorado Tick Fever Virus Strains and Demarcation of a New Species
title_full_unstemmed Genomic Evaluation of the Genus Coltivirus Indicates Genetic Diversity among Colorado Tick Fever Virus Strains and Demarcation of a New Species
title_short Genomic Evaluation of the Genus Coltivirus Indicates Genetic Diversity among Colorado Tick Fever Virus Strains and Demarcation of a New Species
title_sort genomic evaluation of the genus coltivirus indicates genetic diversity among colorado tick fever virus strains and demarcation of a new species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700517/
https://www.ncbi.nlm.nih.gov/pubmed/34940030
http://dx.doi.org/10.3390/diseases9040092
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