Cargando…

Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like

Obesity is associated with chronic low-grade inflammation. The benefits of exercise are partly attributed to its anti-inflammatory effect, but whether exercise can regulate NLRP3 inflammasome activation in obese adipose tissue remains unknown. Meteorin-like (METRNL), a recently discovered myokine, h...

Descripción completa

Detalles Bibliográficos
Autores principales: Javaid, Hafiz Muhammad Ahmad, Sahar, Namood E, ZhuGe, De-Li, Huh, Joo Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700724/
https://www.ncbi.nlm.nih.gov/pubmed/34943988
http://dx.doi.org/10.3390/cells10123480
_version_ 1784620826456227840
author Javaid, Hafiz Muhammad Ahmad
Sahar, Namood E
ZhuGe, De-Li
Huh, Joo Young
author_facet Javaid, Hafiz Muhammad Ahmad
Sahar, Namood E
ZhuGe, De-Li
Huh, Joo Young
author_sort Javaid, Hafiz Muhammad Ahmad
collection PubMed
description Obesity is associated with chronic low-grade inflammation. The benefits of exercise are partly attributed to its anti-inflammatory effect, but whether exercise can regulate NLRP3 inflammasome activation in obese adipose tissue remains unknown. Meteorin-like (METRNL), a recently discovered myokine, has been implicated in mediating the effect of exercise on metabolism. Herein, we examined the effect of exercise and METRNL on NLRP3 inflammasome activation. High-fat diet (HFD)-induced obese mice were subjected to treadmill exercise for 8 weeks. A subgroup of HFD mice was switched to normal chow with the exercise intervention. Exercise and diet attenuated weight gain, fat accumulation, and insulin resistance in obese mice. In addition, exercise downregulated gene and protein levels of inflammasome markers, including NLRP3 and caspase-1, in adipose tissue. In isolated bone marrow-derived macrophages, activation of NLRP3 inflammasome was suppressed in the exercise group, as confirmed by the downregulation of IL-1β and IL-18. Exercise significantly enhanced the expression of METRNL in various muscle depots, and further in vitro analysis revealed that recombinant METRNL treatment inhibited IL-1β secretion in macrophages. In conclusion, exercise exerts its anti-inflammatory action by suppressing adipose tissue NLRP3 inflammasome, and this is, in part, associated with METRNL induction in muscle and its anti-inflammatory effects in macrophages.
format Online
Article
Text
id pubmed-8700724
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87007242021-12-24 Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like Javaid, Hafiz Muhammad Ahmad Sahar, Namood E ZhuGe, De-Li Huh, Joo Young Cells Article Obesity is associated with chronic low-grade inflammation. The benefits of exercise are partly attributed to its anti-inflammatory effect, but whether exercise can regulate NLRP3 inflammasome activation in obese adipose tissue remains unknown. Meteorin-like (METRNL), a recently discovered myokine, has been implicated in mediating the effect of exercise on metabolism. Herein, we examined the effect of exercise and METRNL on NLRP3 inflammasome activation. High-fat diet (HFD)-induced obese mice were subjected to treadmill exercise for 8 weeks. A subgroup of HFD mice was switched to normal chow with the exercise intervention. Exercise and diet attenuated weight gain, fat accumulation, and insulin resistance in obese mice. In addition, exercise downregulated gene and protein levels of inflammasome markers, including NLRP3 and caspase-1, in adipose tissue. In isolated bone marrow-derived macrophages, activation of NLRP3 inflammasome was suppressed in the exercise group, as confirmed by the downregulation of IL-1β and IL-18. Exercise significantly enhanced the expression of METRNL in various muscle depots, and further in vitro analysis revealed that recombinant METRNL treatment inhibited IL-1β secretion in macrophages. In conclusion, exercise exerts its anti-inflammatory action by suppressing adipose tissue NLRP3 inflammasome, and this is, in part, associated with METRNL induction in muscle and its anti-inflammatory effects in macrophages. MDPI 2021-12-09 /pmc/articles/PMC8700724/ /pubmed/34943988 http://dx.doi.org/10.3390/cells10123480 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Javaid, Hafiz Muhammad Ahmad
Sahar, Namood E
ZhuGe, De-Li
Huh, Joo Young
Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like
title Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like
title_full Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like
title_fullStr Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like
title_full_unstemmed Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like
title_short Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like
title_sort exercise inhibits nlrp3 inflammasome activation in obese mice via the anti-inflammatory effect of meteorin-like
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700724/
https://www.ncbi.nlm.nih.gov/pubmed/34943988
http://dx.doi.org/10.3390/cells10123480
work_keys_str_mv AT javaidhafizmuhammadahmad exerciseinhibitsnlrp3inflammasomeactivationinobesemiceviatheantiinflammatoryeffectofmeteorinlike
AT saharnamoode exerciseinhibitsnlrp3inflammasomeactivationinobesemiceviatheantiinflammatoryeffectofmeteorinlike
AT zhugedeli exerciseinhibitsnlrp3inflammasomeactivationinobesemiceviatheantiinflammatoryeffectofmeteorinlike
AT huhjooyoung exerciseinhibitsnlrp3inflammasomeactivationinobesemiceviatheantiinflammatoryeffectofmeteorinlike