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A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection
To achieve the rapid detection of Listeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO(3)) was used as a photosensitive material, was modified with gold nanopar...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8701101/ https://www.ncbi.nlm.nih.gov/pubmed/34945447 http://dx.doi.org/10.3390/foods10122896 |
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author | Zhu, Liangliang Hao, Hongshun Ding, Chao Gan, Hanwei Jiang, Shuting Zhang, Gongliang Bi, Jingran Yan, Shuang Hou, Hongman |
author_facet | Zhu, Liangliang Hao, Hongshun Ding, Chao Gan, Hanwei Jiang, Shuting Zhang, Gongliang Bi, Jingran Yan, Shuang Hou, Hongman |
author_sort | Zhu, Liangliang |
collection | PubMed |
description | To achieve the rapid detection of Listeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO(3)) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 × 10(1)–1.3 × 10(7) CFU/mL. The construction strategy provides a new way to detect Listeria monocytogenes. |
format | Online Article Text |
id | pubmed-8701101 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87011012021-12-24 A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection Zhu, Liangliang Hao, Hongshun Ding, Chao Gan, Hanwei Jiang, Shuting Zhang, Gongliang Bi, Jingran Yan, Shuang Hou, Hongman Foods Article To achieve the rapid detection of Listeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO(3)) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 × 10(1)–1.3 × 10(7) CFU/mL. The construction strategy provides a new way to detect Listeria monocytogenes. MDPI 2021-11-23 /pmc/articles/PMC8701101/ /pubmed/34945447 http://dx.doi.org/10.3390/foods10122896 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhu, Liangliang Hao, Hongshun Ding, Chao Gan, Hanwei Jiang, Shuting Zhang, Gongliang Bi, Jingran Yan, Shuang Hou, Hongman A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection |
title | A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection |
title_full | A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection |
title_fullStr | A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection |
title_full_unstemmed | A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection |
title_short | A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection |
title_sort | novel photoelectrochemical aptamer sensor based on cdte quantum dots enhancement and exonuclease i-assisted signal amplification for listeria monocytogenes detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8701101/ https://www.ncbi.nlm.nih.gov/pubmed/34945447 http://dx.doi.org/10.3390/foods10122896 |
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