Cargando…

Analysis of Peripheral Blood Mononuclear Cells Gene Expression Highlights the Role of Extracellular Vesicles in the Immune Response following Hematopoietic Stem Cell Transplantation in Children

Hematopoietic stem cell transplantation (HSCT) is an effective treatment method used in many neoplastic and non-neoplastic diseases that affect the bone marrow, blood cells, and immune system. The procedure is associated with a risk of adverse events, mostly related to the immune response after tran...

Descripción completa

Detalles Bibliográficos
Autores principales: Strojny, Wojciech, Kwiecińska, Kinga, Hałubiec, Przemysław, Kowalczyk, Wojciech, Miklusiak, Karol, Łazarczyk, Agnieszka, Skoczeń, Szymon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8701260/
https://www.ncbi.nlm.nih.gov/pubmed/34946957
http://dx.doi.org/10.3390/genes12122008
_version_ 1784620957166469120
author Strojny, Wojciech
Kwiecińska, Kinga
Hałubiec, Przemysław
Kowalczyk, Wojciech
Miklusiak, Karol
Łazarczyk, Agnieszka
Skoczeń, Szymon
author_facet Strojny, Wojciech
Kwiecińska, Kinga
Hałubiec, Przemysław
Kowalczyk, Wojciech
Miklusiak, Karol
Łazarczyk, Agnieszka
Skoczeń, Szymon
author_sort Strojny, Wojciech
collection PubMed
description Hematopoietic stem cell transplantation (HSCT) is an effective treatment method used in many neoplastic and non-neoplastic diseases that affect the bone marrow, blood cells, and immune system. The procedure is associated with a risk of adverse events, mostly related to the immune response after transplantation. The aim of our research was to identify genes, processes and cellular entities involved in the variety of changes occurring after allogeneic HSCT in children by performing a whole genome expression assessment together with pathway enrichment analysis. We conducted a prospective study of 27 patients (aged 1.5–18 years) qualified for allogenic HSCT. Blood samples were obtained before HSCT and 6 months after the procedure. Microarrays were used to analyze gene expressions in peripheral blood mononuclear cells. This was followed by Gene Ontology (GO) functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein–protein interaction (PPI) analysis using bioinformatic tools. We found 139 differentially expressed genes (DEGs) of which 91 were upregulated and 48 were downregulated. “Blood microparticle”, “extracellular exosome”, “B-cell receptor signaling pathway”, “complement activation” and “antigen binding” were among GO terms found to be significantly enriched. The PPI analysis identified 16 hub genes. Our results provide insight into a broad spectrum of epigenetic changes that occur after HSCT. In particular, they further highlight the importance of extracellular vesicles (exosomes and microparticles) in the post-HSCT immune response.
format Online
Article
Text
id pubmed-8701260
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87012602021-12-24 Analysis of Peripheral Blood Mononuclear Cells Gene Expression Highlights the Role of Extracellular Vesicles in the Immune Response following Hematopoietic Stem Cell Transplantation in Children Strojny, Wojciech Kwiecińska, Kinga Hałubiec, Przemysław Kowalczyk, Wojciech Miklusiak, Karol Łazarczyk, Agnieszka Skoczeń, Szymon Genes (Basel) Article Hematopoietic stem cell transplantation (HSCT) is an effective treatment method used in many neoplastic and non-neoplastic diseases that affect the bone marrow, blood cells, and immune system. The procedure is associated with a risk of adverse events, mostly related to the immune response after transplantation. The aim of our research was to identify genes, processes and cellular entities involved in the variety of changes occurring after allogeneic HSCT in children by performing a whole genome expression assessment together with pathway enrichment analysis. We conducted a prospective study of 27 patients (aged 1.5–18 years) qualified for allogenic HSCT. Blood samples were obtained before HSCT and 6 months after the procedure. Microarrays were used to analyze gene expressions in peripheral blood mononuclear cells. This was followed by Gene Ontology (GO) functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein–protein interaction (PPI) analysis using bioinformatic tools. We found 139 differentially expressed genes (DEGs) of which 91 were upregulated and 48 were downregulated. “Blood microparticle”, “extracellular exosome”, “B-cell receptor signaling pathway”, “complement activation” and “antigen binding” were among GO terms found to be significantly enriched. The PPI analysis identified 16 hub genes. Our results provide insight into a broad spectrum of epigenetic changes that occur after HSCT. In particular, they further highlight the importance of extracellular vesicles (exosomes and microparticles) in the post-HSCT immune response. MDPI 2021-12-17 /pmc/articles/PMC8701260/ /pubmed/34946957 http://dx.doi.org/10.3390/genes12122008 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Strojny, Wojciech
Kwiecińska, Kinga
Hałubiec, Przemysław
Kowalczyk, Wojciech
Miklusiak, Karol
Łazarczyk, Agnieszka
Skoczeń, Szymon
Analysis of Peripheral Blood Mononuclear Cells Gene Expression Highlights the Role of Extracellular Vesicles in the Immune Response following Hematopoietic Stem Cell Transplantation in Children
title Analysis of Peripheral Blood Mononuclear Cells Gene Expression Highlights the Role of Extracellular Vesicles in the Immune Response following Hematopoietic Stem Cell Transplantation in Children
title_full Analysis of Peripheral Blood Mononuclear Cells Gene Expression Highlights the Role of Extracellular Vesicles in the Immune Response following Hematopoietic Stem Cell Transplantation in Children
title_fullStr Analysis of Peripheral Blood Mononuclear Cells Gene Expression Highlights the Role of Extracellular Vesicles in the Immune Response following Hematopoietic Stem Cell Transplantation in Children
title_full_unstemmed Analysis of Peripheral Blood Mononuclear Cells Gene Expression Highlights the Role of Extracellular Vesicles in the Immune Response following Hematopoietic Stem Cell Transplantation in Children
title_short Analysis of Peripheral Blood Mononuclear Cells Gene Expression Highlights the Role of Extracellular Vesicles in the Immune Response following Hematopoietic Stem Cell Transplantation in Children
title_sort analysis of peripheral blood mononuclear cells gene expression highlights the role of extracellular vesicles in the immune response following hematopoietic stem cell transplantation in children
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8701260/
https://www.ncbi.nlm.nih.gov/pubmed/34946957
http://dx.doi.org/10.3390/genes12122008
work_keys_str_mv AT strojnywojciech analysisofperipheralbloodmononuclearcellsgeneexpressionhighlightstheroleofextracellularvesiclesintheimmuneresponsefollowinghematopoieticstemcelltransplantationinchildren
AT kwiecinskakinga analysisofperipheralbloodmononuclearcellsgeneexpressionhighlightstheroleofextracellularvesiclesintheimmuneresponsefollowinghematopoieticstemcelltransplantationinchildren
AT hałubiecprzemysław analysisofperipheralbloodmononuclearcellsgeneexpressionhighlightstheroleofextracellularvesiclesintheimmuneresponsefollowinghematopoieticstemcelltransplantationinchildren
AT kowalczykwojciech analysisofperipheralbloodmononuclearcellsgeneexpressionhighlightstheroleofextracellularvesiclesintheimmuneresponsefollowinghematopoieticstemcelltransplantationinchildren
AT miklusiakkarol analysisofperipheralbloodmononuclearcellsgeneexpressionhighlightstheroleofextracellularvesiclesintheimmuneresponsefollowinghematopoieticstemcelltransplantationinchildren
AT łazarczykagnieszka analysisofperipheralbloodmononuclearcellsgeneexpressionhighlightstheroleofextracellularvesiclesintheimmuneresponsefollowinghematopoieticstemcelltransplantationinchildren
AT skoczenszymon analysisofperipheralbloodmononuclearcellsgeneexpressionhighlightstheroleofextracellularvesiclesintheimmuneresponsefollowinghematopoieticstemcelltransplantationinchildren