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The Evolution and Expression Profiles of EC1 Gene Family during Development in Cotton
Fertilization is essential to sexual reproduction of flowering plants. EC1 (EGG CELL 1) proteins have a conserved cysteine spacer characteristic and play a crucial role in double fertilization process in many plant species. However, to date, the role of EC1 gene family in cotton is fully unknown. He...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702097/ https://www.ncbi.nlm.nih.gov/pubmed/34946950 http://dx.doi.org/10.3390/genes12122001 |
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author | Wang, Xinyu Chen, Wei Yao, Jinbo Li, Yan Yeboah, Akwasi Zhu, Shouhong Zhang, Yongshan |
author_facet | Wang, Xinyu Chen, Wei Yao, Jinbo Li, Yan Yeboah, Akwasi Zhu, Shouhong Zhang, Yongshan |
author_sort | Wang, Xinyu |
collection | PubMed |
description | Fertilization is essential to sexual reproduction of flowering plants. EC1 (EGG CELL 1) proteins have a conserved cysteine spacer characteristic and play a crucial role in double fertilization process in many plant species. However, to date, the role of EC1 gene family in cotton is fully unknown. Hence, detailed bioinformatics analysis was explored to elucidate the biological mechanisms of EC1 gene family in cotton. In this study, we identified 66 genes in 10 plant species in which a total of 39 EC1 genes were detected from cotton genome. Phylogenetic analysis clustered the identified EC1 genes into three families (I-III) and all of them contain Prolamin-like domains. A good collinearity was observed in the synteny analysis of the orthologs from cotton genomes. Whole-genome duplication was determined to be one of the major impetuses for the expansion of the EC1 gene family during the process of evolution. qRT-PCR analysis showed that EC1 genes were highly expressed in reproductive tissues under multiple stresses, signifying their potential role in enhancing stress tolerance or responses. Additionally, gene interaction networks showed that EC1 genes may be involved in cell stress and response transcriptional regulator in the synergid cells and activate the expression of genes required for pollen tube guidance. Our results provide novel functional insights into the evolution and functional elucidation of EC1 gene family in cotton. |
format | Online Article Text |
id | pubmed-8702097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87020972021-12-24 The Evolution and Expression Profiles of EC1 Gene Family during Development in Cotton Wang, Xinyu Chen, Wei Yao, Jinbo Li, Yan Yeboah, Akwasi Zhu, Shouhong Zhang, Yongshan Genes (Basel) Article Fertilization is essential to sexual reproduction of flowering plants. EC1 (EGG CELL 1) proteins have a conserved cysteine spacer characteristic and play a crucial role in double fertilization process in many plant species. However, to date, the role of EC1 gene family in cotton is fully unknown. Hence, detailed bioinformatics analysis was explored to elucidate the biological mechanisms of EC1 gene family in cotton. In this study, we identified 66 genes in 10 plant species in which a total of 39 EC1 genes were detected from cotton genome. Phylogenetic analysis clustered the identified EC1 genes into three families (I-III) and all of them contain Prolamin-like domains. A good collinearity was observed in the synteny analysis of the orthologs from cotton genomes. Whole-genome duplication was determined to be one of the major impetuses for the expansion of the EC1 gene family during the process of evolution. qRT-PCR analysis showed that EC1 genes were highly expressed in reproductive tissues under multiple stresses, signifying their potential role in enhancing stress tolerance or responses. Additionally, gene interaction networks showed that EC1 genes may be involved in cell stress and response transcriptional regulator in the synergid cells and activate the expression of genes required for pollen tube guidance. Our results provide novel functional insights into the evolution and functional elucidation of EC1 gene family in cotton. MDPI 2021-12-17 /pmc/articles/PMC8702097/ /pubmed/34946950 http://dx.doi.org/10.3390/genes12122001 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Xinyu Chen, Wei Yao, Jinbo Li, Yan Yeboah, Akwasi Zhu, Shouhong Zhang, Yongshan The Evolution and Expression Profiles of EC1 Gene Family during Development in Cotton |
title | The Evolution and Expression Profiles of EC1 Gene Family during Development in Cotton |
title_full | The Evolution and Expression Profiles of EC1 Gene Family during Development in Cotton |
title_fullStr | The Evolution and Expression Profiles of EC1 Gene Family during Development in Cotton |
title_full_unstemmed | The Evolution and Expression Profiles of EC1 Gene Family during Development in Cotton |
title_short | The Evolution and Expression Profiles of EC1 Gene Family during Development in Cotton |
title_sort | evolution and expression profiles of ec1 gene family during development in cotton |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702097/ https://www.ncbi.nlm.nih.gov/pubmed/34946950 http://dx.doi.org/10.3390/genes12122001 |
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