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A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

Salmonella enterica subsp. enterica serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of...

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Autores principales: MA, ZHONGMEI, LI, NA, NING, CHENGCHENG, LIU, YUCHENG, GUO, YUN, JI, CHUNHUI, ZHU, XIAOZHEN, MENG, QINGLING, XIA, XIANZHU, ZHANG, XINGXING, CAI, XUEPENG, CAI, KUOJUN, JUN, QIAO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702606/
https://www.ncbi.nlm.nih.gov/pubmed/35003279
http://dx.doi.org/10.33073/pjm-2021-045
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author MA, ZHONGMEI
LI, NA
NING, CHENGCHENG
LIU, YUCHENG
GUO, YUN
JI, CHUNHUI
ZHU, XIAOZHEN
MENG, QINGLING
XIA, XIANZHU
ZHANG, XINGXING
CAI, XUEPENG
CAI, KUOJUN
JUN, QIAO
author_facet MA, ZHONGMEI
LI, NA
NING, CHENGCHENG
LIU, YUCHENG
GUO, YUN
JI, CHUNHUI
ZHU, XIAOZHEN
MENG, QINGLING
XIA, XIANZHU
ZHANG, XINGXING
CAI, XUEPENG
CAI, KUOJUN
JUN, QIAO
author_sort MA, ZHONGMEI
collection PubMed
description Salmonella enterica subsp. enterica serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of ST to environmental stress and biofilm formation, the STM0859 gene-deficient strain and the complementation strain ΔSTM0859/STM0859 were generated, respectively. Then, its capacity of responding to environmental stresses and biofilm (BF) formation ability under different stresses, including acid, alkali, high salt, cholate, and oxidative stresses was tested. We further analyzed the interaction between the STM0859 protein and the promoter of the acid stress response-related gene rcsB by performing an electrophoresis mobility shift assay (EMSA). The results showed that acid resistance and BF formation capacities of ST-ΔSTM0859 strain were significantly weaker, as compared with those of Salmonella Typhimurium SL1344 (ST-SL1344) wild strain (p < 0.01). Quantitative qRT-PCR analysis showed that the expression levels of acid stress and BF formation-related genes, rcsB and rpoS, of ST-ΔSTM0859 strain were significantly reduced at the transcription levels, while the transcription levels of these genes were fully restored in complementation strain ST-ΔSTM0859/STM0859. The results of EMSA showed that STM0859 was capable of binding the promoter DNA fragments of the rcsB gene, suggesting that STM0859 can promote the transcription of the rcsB gene through interaction with its promoter, thereby exerting an indirectly regulatory role on the adaptive responses to acid stress and BF formation of ST. This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.
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spelling pubmed-87026062022-01-06 A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation MA, ZHONGMEI LI, NA NING, CHENGCHENG LIU, YUCHENG GUO, YUN JI, CHUNHUI ZHU, XIAOZHEN MENG, QINGLING XIA, XIANZHU ZHANG, XINGXING CAI, XUEPENG CAI, KUOJUN JUN, QIAO Pol J Microbiol Microbiology Salmonella enterica subsp. enterica serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of ST to environmental stress and biofilm formation, the STM0859 gene-deficient strain and the complementation strain ΔSTM0859/STM0859 were generated, respectively. Then, its capacity of responding to environmental stresses and biofilm (BF) formation ability under different stresses, including acid, alkali, high salt, cholate, and oxidative stresses was tested. We further analyzed the interaction between the STM0859 protein and the promoter of the acid stress response-related gene rcsB by performing an electrophoresis mobility shift assay (EMSA). The results showed that acid resistance and BF formation capacities of ST-ΔSTM0859 strain were significantly weaker, as compared with those of Salmonella Typhimurium SL1344 (ST-SL1344) wild strain (p < 0.01). Quantitative qRT-PCR analysis showed that the expression levels of acid stress and BF formation-related genes, rcsB and rpoS, of ST-ΔSTM0859 strain were significantly reduced at the transcription levels, while the transcription levels of these genes were fully restored in complementation strain ST-ΔSTM0859/STM0859. The results of EMSA showed that STM0859 was capable of binding the promoter DNA fragments of the rcsB gene, suggesting that STM0859 can promote the transcription of the rcsB gene through interaction with its promoter, thereby exerting an indirectly regulatory role on the adaptive responses to acid stress and BF formation of ST. This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses. Exeley Inc. 2021-12 2021-12-23 /pmc/articles/PMC8702606/ /pubmed/35003279 http://dx.doi.org/10.33073/pjm-2021-045 Text en © 2021 Zhongmei Ma et al https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Microbiology
MA, ZHONGMEI
LI, NA
NING, CHENGCHENG
LIU, YUCHENG
GUO, YUN
JI, CHUNHUI
ZHU, XIAOZHEN
MENG, QINGLING
XIA, XIANZHU
ZHANG, XINGXING
CAI, XUEPENG
CAI, KUOJUN
JUN, QIAO
A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation
title A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation
title_full A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation
title_fullStr A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation
title_full_unstemmed A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation
title_short A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation
title_sort novel lysr family factor stm0859 is associated with the responses of salmonella typhimurium to environmental stress and biofilm formation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702606/
https://www.ncbi.nlm.nih.gov/pubmed/35003279
http://dx.doi.org/10.33073/pjm-2021-045
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