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Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells
Microbe-associated molecular patterns, such as lipopolysaccharide (LPS) and β-glucan (BG), are surrogates of immune challenges like bacterial and fungal infections, respectively. The biologically active form of vitamin D, 1α,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), supports the immune system in i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702862/ https://www.ncbi.nlm.nih.gov/pubmed/34956186 http://dx.doi.org/10.3389/fimmu.2021.754056 |
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author | Malmberg, Henna-Riikka Hanel, Andrea Taipale, Mari Heikkinen, Sami Carlberg, Carsten |
author_facet | Malmberg, Henna-Riikka Hanel, Andrea Taipale, Mari Heikkinen, Sami Carlberg, Carsten |
author_sort | Malmberg, Henna-Riikka |
collection | PubMed |
description | Microbe-associated molecular patterns, such as lipopolysaccharide (LPS) and β-glucan (BG), are surrogates of immune challenges like bacterial and fungal infections, respectively. The biologically active form of vitamin D, 1α,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), supports the immune system in its fight against infections. This study investigated significant and prominent changes of the transcriptome of human peripheral blood mononuclear cells that immediately after isolation are exposed to 1,25(OH)(2)D(3)-modulated immune challenges over a time frame of 24-48 h. In this in vitro study design, most LPS and BG responsive genes are downregulated and their counts are drastically reduced when cells are treated 24 h after, 24 h before or in parallel with 1,25(OH)(2)D(3). Interestingly, only a 1,25(OH)(2)D(3) pre-treatment of the LPS challenge results in a majority of upregulated genes. Based on transcriptome-wide data both immune challenges display characteristic differences in responsive genes and their associated pathways, to which the actions of 1,25(OH)(2)D(3) often oppose. The joined BG/1,25(OH)(2)D(3) response is less sensitive to treatment sequence than that of LPS/1,25(OH)(2)D(3). In conclusion, the functional consequences of immune challenges are significantly modulated by 1,25(OH)(2)D(3) but largely depend on treatment sequence. This may suggest that a sufficient vitamin D status before an infection is more important than vitamin D supplementation afterwards. |
format | Online Article Text |
id | pubmed-8702862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87028622021-12-25 Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells Malmberg, Henna-Riikka Hanel, Andrea Taipale, Mari Heikkinen, Sami Carlberg, Carsten Front Immunol Immunology Microbe-associated molecular patterns, such as lipopolysaccharide (LPS) and β-glucan (BG), are surrogates of immune challenges like bacterial and fungal infections, respectively. The biologically active form of vitamin D, 1α,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), supports the immune system in its fight against infections. This study investigated significant and prominent changes of the transcriptome of human peripheral blood mononuclear cells that immediately after isolation are exposed to 1,25(OH)(2)D(3)-modulated immune challenges over a time frame of 24-48 h. In this in vitro study design, most LPS and BG responsive genes are downregulated and their counts are drastically reduced when cells are treated 24 h after, 24 h before or in parallel with 1,25(OH)(2)D(3). Interestingly, only a 1,25(OH)(2)D(3) pre-treatment of the LPS challenge results in a majority of upregulated genes. Based on transcriptome-wide data both immune challenges display characteristic differences in responsive genes and their associated pathways, to which the actions of 1,25(OH)(2)D(3) often oppose. The joined BG/1,25(OH)(2)D(3) response is less sensitive to treatment sequence than that of LPS/1,25(OH)(2)D(3). In conclusion, the functional consequences of immune challenges are significantly modulated by 1,25(OH)(2)D(3) but largely depend on treatment sequence. This may suggest that a sufficient vitamin D status before an infection is more important than vitamin D supplementation afterwards. Frontiers Media S.A. 2021-12-10 /pmc/articles/PMC8702862/ /pubmed/34956186 http://dx.doi.org/10.3389/fimmu.2021.754056 Text en Copyright © 2021 Malmberg, Hanel, Taipale, Heikkinen and Carlberg https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Malmberg, Henna-Riikka Hanel, Andrea Taipale, Mari Heikkinen, Sami Carlberg, Carsten Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells |
title | Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells |
title_full | Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells |
title_fullStr | Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells |
title_full_unstemmed | Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells |
title_short | Vitamin D Treatment Sequence Is Critical for Transcriptome Modulation of Immune Challenged Primary Human Cells |
title_sort | vitamin d treatment sequence is critical for transcriptome modulation of immune challenged primary human cells |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702862/ https://www.ncbi.nlm.nih.gov/pubmed/34956186 http://dx.doi.org/10.3389/fimmu.2021.754056 |
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