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Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge

Salmonella enterica serovar Enteritidis is a bacterial pathogen that contributes to poultry production losses and human foodborne illness. The bacterium elicits a broad immune response involving both the innate and adaptive components of the immune system. Coordination of the immune response is larg...

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Autores principales: Kaiser, Michael G., Hsieh, John, Kaiser, Pete, Lamont, Susan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703071/
https://www.ncbi.nlm.nih.gov/pubmed/34936953
http://dx.doi.org/10.1016/j.psj.2021.101605
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author Kaiser, Michael G.
Hsieh, John
Kaiser, Pete
Lamont, Susan J.
author_facet Kaiser, Michael G.
Hsieh, John
Kaiser, Pete
Lamont, Susan J.
author_sort Kaiser, Michael G.
collection PubMed
description Salmonella enterica serovar Enteritidis is a bacterial pathogen that contributes to poultry production losses and human foodborne illness. The bacterium elicits a broad immune response involving both the innate and adaptive components of the immune system. Coordination of the immune response is largely directed by cytokines. The objective of the current study was to characterize the expression of a select set of cytokines and regulatory immune genes in three genetically diverse chicken lines after infection with S. Enteritidis. Leghorn, Fayoumi and broiler day-old chicks were orally infected with pathogenic S. Enteritidis or culture medium. At 2 and 18 h postinfection, spleens and ceca were collected and mRNA expression levels for 7 genes (GM-CSF, IL2, IL15, TGF-β1, SOCS3, P20K, and MHC class IIβ) were evaluated by real-time quantitative PCR. Genetic line had a significant effect on mRNA expression levels of IL15, TGF-β1, SOCS3 and P20K in the spleen and on P20K and MHC class IIβ in the cecum. Comparing challenged vs. unchallenged birds, the expression of SOCS3 and P20K mRNA were significantly higher in the spleen and cecum, while MHC class IIβ mRNA was significantly lower in spleen. Combining the current RNA expression results with those of previously reported studies on the same samples reveals distinct RNA expression profiles among the three genetic chicken lines and the 2 tissues. This study illustrates that these diverse genetic lines have distinctively different immune response to S. Enteritidis challenge within the spleen and the cecum.
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spelling pubmed-87030712022-01-04 Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge Kaiser, Michael G. Hsieh, John Kaiser, Pete Lamont, Susan J. Poult Sci IMMUNOLOGY, HEALTH AND DISEASE Salmonella enterica serovar Enteritidis is a bacterial pathogen that contributes to poultry production losses and human foodborne illness. The bacterium elicits a broad immune response involving both the innate and adaptive components of the immune system. Coordination of the immune response is largely directed by cytokines. The objective of the current study was to characterize the expression of a select set of cytokines and regulatory immune genes in three genetically diverse chicken lines after infection with S. Enteritidis. Leghorn, Fayoumi and broiler day-old chicks were orally infected with pathogenic S. Enteritidis or culture medium. At 2 and 18 h postinfection, spleens and ceca were collected and mRNA expression levels for 7 genes (GM-CSF, IL2, IL15, TGF-β1, SOCS3, P20K, and MHC class IIβ) were evaluated by real-time quantitative PCR. Genetic line had a significant effect on mRNA expression levels of IL15, TGF-β1, SOCS3 and P20K in the spleen and on P20K and MHC class IIβ in the cecum. Comparing challenged vs. unchallenged birds, the expression of SOCS3 and P20K mRNA were significantly higher in the spleen and cecum, while MHC class IIβ mRNA was significantly lower in spleen. Combining the current RNA expression results with those of previously reported studies on the same samples reveals distinct RNA expression profiles among the three genetic chicken lines and the 2 tissues. This study illustrates that these diverse genetic lines have distinctively different immune response to S. Enteritidis challenge within the spleen and the cecum. Elsevier 2021-11-19 /pmc/articles/PMC8703071/ /pubmed/34936953 http://dx.doi.org/10.1016/j.psj.2021.101605 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Kaiser, Michael G.
Hsieh, John
Kaiser, Pete
Lamont, Susan J.
Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge
title Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge
title_full Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge
title_fullStr Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge
title_full_unstemmed Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge
title_short Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge
title_sort differential immunological response detected in mrna expression profiles among diverse chicken lines in response to salmonella challenge
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703071/
https://www.ncbi.nlm.nih.gov/pubmed/34936953
http://dx.doi.org/10.1016/j.psj.2021.101605
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