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Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing

The Yaqui Valley, Mexico, has been historically considered as an experimental field for semiarid regions worldwide since temperature is an important constraint affecting durum wheat cultivation. Here, we studied the transcriptional and morphometrical response of durum wheat at an increased temperatu...

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Autores principales: Chaparro-Encinas, Luis Abraham, Santoyo, Gustavo, Peña-Cabriales, Juan José, Castro-Espinoza, Luciano, Parra-Cota, Fannie Isela, Santos-Villalobos, Sergio de los
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703274/
https://www.ncbi.nlm.nih.gov/pubmed/34961263
http://dx.doi.org/10.3390/plants10122792
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author Chaparro-Encinas, Luis Abraham
Santoyo, Gustavo
Peña-Cabriales, Juan José
Castro-Espinoza, Luciano
Parra-Cota, Fannie Isela
Santos-Villalobos, Sergio de los
author_facet Chaparro-Encinas, Luis Abraham
Santoyo, Gustavo
Peña-Cabriales, Juan José
Castro-Espinoza, Luciano
Parra-Cota, Fannie Isela
Santos-Villalobos, Sergio de los
author_sort Chaparro-Encinas, Luis Abraham
collection PubMed
description The Yaqui Valley, Mexico, has been historically considered as an experimental field for semiarid regions worldwide since temperature is an important constraint affecting durum wheat cultivation. Here, we studied the transcriptional and morphometrical response of durum wheat at an increased temperature (+2 °C) for deciphering molecular mechanisms involved in the thermal adaptation by this crop. The morphometrical assay showed a significant decrease in almost all the evaluated traits (shoot/root length, biovolume index, and dry/shoot weight) except in the dry root weight and the root:shoot ratio. At the transcriptional level, 283 differentially expressed genes (DEGs) were obtained (False Discovery Rate (FDR) ≤ 0.05 and |log2 fold change| ≥ 1.3). From these, functional annotation with MapMan4 and a gene ontology (GO) enrichment analysis with GOSeq were carried out to obtain 27 GO terms significantly enriched (overrepresented FDR ≤ 0.05). Overrepresented and functionally annotated genes belonged to ontologies associated with photosynthetic acclimation, respiration, changes in carbon balance, lipid biosynthesis, the regulation of reactive oxygen species, and the acceleration of physiological progression. These findings are the first insight into the regulation of the mechanism influenced by a temperature increase in durum wheat.
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spelling pubmed-87032742021-12-25 Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing Chaparro-Encinas, Luis Abraham Santoyo, Gustavo Peña-Cabriales, Juan José Castro-Espinoza, Luciano Parra-Cota, Fannie Isela Santos-Villalobos, Sergio de los Plants (Basel) Article The Yaqui Valley, Mexico, has been historically considered as an experimental field for semiarid regions worldwide since temperature is an important constraint affecting durum wheat cultivation. Here, we studied the transcriptional and morphometrical response of durum wheat at an increased temperature (+2 °C) for deciphering molecular mechanisms involved in the thermal adaptation by this crop. The morphometrical assay showed a significant decrease in almost all the evaluated traits (shoot/root length, biovolume index, and dry/shoot weight) except in the dry root weight and the root:shoot ratio. At the transcriptional level, 283 differentially expressed genes (DEGs) were obtained (False Discovery Rate (FDR) ≤ 0.05 and |log2 fold change| ≥ 1.3). From these, functional annotation with MapMan4 and a gene ontology (GO) enrichment analysis with GOSeq were carried out to obtain 27 GO terms significantly enriched (overrepresented FDR ≤ 0.05). Overrepresented and functionally annotated genes belonged to ontologies associated with photosynthetic acclimation, respiration, changes in carbon balance, lipid biosynthesis, the regulation of reactive oxygen species, and the acceleration of physiological progression. These findings are the first insight into the regulation of the mechanism influenced by a temperature increase in durum wheat. MDPI 2021-12-16 /pmc/articles/PMC8703274/ /pubmed/34961263 http://dx.doi.org/10.3390/plants10122792 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chaparro-Encinas, Luis Abraham
Santoyo, Gustavo
Peña-Cabriales, Juan José
Castro-Espinoza, Luciano
Parra-Cota, Fannie Isela
Santos-Villalobos, Sergio de los
Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing
title Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing
title_full Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing
title_fullStr Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing
title_full_unstemmed Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing
title_short Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing
title_sort transcriptional regulation of metabolic and cellular processes in durum wheat (triticum turgidum subsp. durum) in the face of temperature increasing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703274/
https://www.ncbi.nlm.nih.gov/pubmed/34961263
http://dx.doi.org/10.3390/plants10122792
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