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Highly Efficient Multi-Step Oxidation Bioanode Using Microfluidic Channels

With the rapid decline of fossil fuels, various types of biofuel cells (BFCs) are being developed as an alternative energy source. BFCs based on multi-enzyme cascade reactions are utilized to extract more electrons from substrates. Thus, more power density is obtained from a single molucule of subst...

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Autores principales: Komatsu, Tomohiro, Hishii, Kazuki, Kimura, Michiko, Amaya, Satoshi, Sakamoto, Hiroaki, Takamura, Eiichiro, Satomura, Takenori, Suye, Shin-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703374/
https://www.ncbi.nlm.nih.gov/pubmed/34948296
http://dx.doi.org/10.3390/ijms222413503
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author Komatsu, Tomohiro
Hishii, Kazuki
Kimura, Michiko
Amaya, Satoshi
Sakamoto, Hiroaki
Takamura, Eiichiro
Satomura, Takenori
Suye, Shin-ichiro
author_facet Komatsu, Tomohiro
Hishii, Kazuki
Kimura, Michiko
Amaya, Satoshi
Sakamoto, Hiroaki
Takamura, Eiichiro
Satomura, Takenori
Suye, Shin-ichiro
author_sort Komatsu, Tomohiro
collection PubMed
description With the rapid decline of fossil fuels, various types of biofuel cells (BFCs) are being developed as an alternative energy source. BFCs based on multi-enzyme cascade reactions are utilized to extract more electrons from substrates. Thus, more power density is obtained from a single molucule of substrate. In the present study, a bioanode that could extract six electrons from a single molecule of L-proline via a three-enzyme cascade reaction was developed and investigated for its possible use in BFCs. These enzymes were immobilized on the electrode to ensure highly efficient electron transfer. Then, oriented immobilization of enzymes was achieved using two types of self-assembled monolayers (SAMs). In addition, a microfluidic system was incorporated to achieve efficient electron transfer. The microfluidic system, in which the electrodes were arranged in a tooth-shaped comb, allowed for substrates to be supplied continuously to the cascade, which resulted in smooth electron transfer. Finally, we developed a high-performance bioanode which resulted in the accumulation of higher current density compared to that of a gold disc electrode (205.8 μA cm(−2): approximately 187 times higher). This presents an opportunity for using the bioanode to develop high-performance BFCs in the future.
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spelling pubmed-87033742021-12-25 Highly Efficient Multi-Step Oxidation Bioanode Using Microfluidic Channels Komatsu, Tomohiro Hishii, Kazuki Kimura, Michiko Amaya, Satoshi Sakamoto, Hiroaki Takamura, Eiichiro Satomura, Takenori Suye, Shin-ichiro Int J Mol Sci Article With the rapid decline of fossil fuels, various types of biofuel cells (BFCs) are being developed as an alternative energy source. BFCs based on multi-enzyme cascade reactions are utilized to extract more electrons from substrates. Thus, more power density is obtained from a single molucule of substrate. In the present study, a bioanode that could extract six electrons from a single molecule of L-proline via a three-enzyme cascade reaction was developed and investigated for its possible use in BFCs. These enzymes were immobilized on the electrode to ensure highly efficient electron transfer. Then, oriented immobilization of enzymes was achieved using two types of self-assembled monolayers (SAMs). In addition, a microfluidic system was incorporated to achieve efficient electron transfer. The microfluidic system, in which the electrodes were arranged in a tooth-shaped comb, allowed for substrates to be supplied continuously to the cascade, which resulted in smooth electron transfer. Finally, we developed a high-performance bioanode which resulted in the accumulation of higher current density compared to that of a gold disc electrode (205.8 μA cm(−2): approximately 187 times higher). This presents an opportunity for using the bioanode to develop high-performance BFCs in the future. MDPI 2021-12-16 /pmc/articles/PMC8703374/ /pubmed/34948296 http://dx.doi.org/10.3390/ijms222413503 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Komatsu, Tomohiro
Hishii, Kazuki
Kimura, Michiko
Amaya, Satoshi
Sakamoto, Hiroaki
Takamura, Eiichiro
Satomura, Takenori
Suye, Shin-ichiro
Highly Efficient Multi-Step Oxidation Bioanode Using Microfluidic Channels
title Highly Efficient Multi-Step Oxidation Bioanode Using Microfluidic Channels
title_full Highly Efficient Multi-Step Oxidation Bioanode Using Microfluidic Channels
title_fullStr Highly Efficient Multi-Step Oxidation Bioanode Using Microfluidic Channels
title_full_unstemmed Highly Efficient Multi-Step Oxidation Bioanode Using Microfluidic Channels
title_short Highly Efficient Multi-Step Oxidation Bioanode Using Microfluidic Channels
title_sort highly efficient multi-step oxidation bioanode using microfluidic channels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703374/
https://www.ncbi.nlm.nih.gov/pubmed/34948296
http://dx.doi.org/10.3390/ijms222413503
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