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Encapsidation of Staufen-2 Enhances Infectivity of HIV-1

Staufen, the RNA-binding family of proteins, affects various steps in the Human Immuno-Deficiency Virus (HIV-1) replication cycle. While our previous study established Staufen-2–HIV-1 Rev interaction and its role in augmenting nucleocytoplasmic export of RRE-containing viral RNA, viral incorporation...

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Autores principales: Balakrishnan, Kannan, Jaguva Vasudevan, Ananda Ayyappan, Mohareer, Krishnaveni, Luedde, Tom, Münk, Carsten, Banerjee, Sharmistha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703407/
https://www.ncbi.nlm.nih.gov/pubmed/34960728
http://dx.doi.org/10.3390/v13122459
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author Balakrishnan, Kannan
Jaguva Vasudevan, Ananda Ayyappan
Mohareer, Krishnaveni
Luedde, Tom
Münk, Carsten
Banerjee, Sharmistha
author_facet Balakrishnan, Kannan
Jaguva Vasudevan, Ananda Ayyappan
Mohareer, Krishnaveni
Luedde, Tom
Münk, Carsten
Banerjee, Sharmistha
author_sort Balakrishnan, Kannan
collection PubMed
description Staufen, the RNA-binding family of proteins, affects various steps in the Human Immuno-Deficiency Virus (HIV-1) replication cycle. While our previous study established Staufen-2–HIV-1 Rev interaction and its role in augmenting nucleocytoplasmic export of RRE-containing viral RNA, viral incorporation of Staufen-2 and its effect on viral propagation were unknown. Here, we report that Staufen-2 interacts with HIV-1 Gag and is incorporated into virions and that encapsidated Staufen-2 boosted viral infectivity. Further, Staufen-2 gets co-packaged into virions, possibly by interacting with host factors Staufen-1 or antiviral protein APOBEC3G, which resulted in different outcomes on the infectivity of Staufen-2-encapsidated virions. These observations suggest that encapsidated host factors influence viral population dynamics and infectivity. With the explicit identification of the incorporation of Staufen proteins into HIV-1 and other retroviruses, such as Simian Immunodeficiency Virus (SIV), we propose that packaging of RNA binding proteins, such as Staufen, in budding virions of retroviruses is probably a general phenomenon that can drive or impact the viral population dynamics, infectivity, and evolution.
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spelling pubmed-87034072021-12-25 Encapsidation of Staufen-2 Enhances Infectivity of HIV-1 Balakrishnan, Kannan Jaguva Vasudevan, Ananda Ayyappan Mohareer, Krishnaveni Luedde, Tom Münk, Carsten Banerjee, Sharmistha Viruses Article Staufen, the RNA-binding family of proteins, affects various steps in the Human Immuno-Deficiency Virus (HIV-1) replication cycle. While our previous study established Staufen-2–HIV-1 Rev interaction and its role in augmenting nucleocytoplasmic export of RRE-containing viral RNA, viral incorporation of Staufen-2 and its effect on viral propagation were unknown. Here, we report that Staufen-2 interacts with HIV-1 Gag and is incorporated into virions and that encapsidated Staufen-2 boosted viral infectivity. Further, Staufen-2 gets co-packaged into virions, possibly by interacting with host factors Staufen-1 or antiviral protein APOBEC3G, which resulted in different outcomes on the infectivity of Staufen-2-encapsidated virions. These observations suggest that encapsidated host factors influence viral population dynamics and infectivity. With the explicit identification of the incorporation of Staufen proteins into HIV-1 and other retroviruses, such as Simian Immunodeficiency Virus (SIV), we propose that packaging of RNA binding proteins, such as Staufen, in budding virions of retroviruses is probably a general phenomenon that can drive or impact the viral population dynamics, infectivity, and evolution. MDPI 2021-12-08 /pmc/articles/PMC8703407/ /pubmed/34960728 http://dx.doi.org/10.3390/v13122459 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Balakrishnan, Kannan
Jaguva Vasudevan, Ananda Ayyappan
Mohareer, Krishnaveni
Luedde, Tom
Münk, Carsten
Banerjee, Sharmistha
Encapsidation of Staufen-2 Enhances Infectivity of HIV-1
title Encapsidation of Staufen-2 Enhances Infectivity of HIV-1
title_full Encapsidation of Staufen-2 Enhances Infectivity of HIV-1
title_fullStr Encapsidation of Staufen-2 Enhances Infectivity of HIV-1
title_full_unstemmed Encapsidation of Staufen-2 Enhances Infectivity of HIV-1
title_short Encapsidation of Staufen-2 Enhances Infectivity of HIV-1
title_sort encapsidation of staufen-2 enhances infectivity of hiv-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703407/
https://www.ncbi.nlm.nih.gov/pubmed/34960728
http://dx.doi.org/10.3390/v13122459
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