Cargando…

An In Vitro Approach to Studying the Microbial Community and Impact of Pre and Probiotics under Anorexia Nervosa Related Dietary Restrictions

Individuals with anorexia nervosa (AN) often suffer psychological and gastrointestinal problems consistent with a dysregulated gut microbial community. Psychobiotics have been postulated to modify microbiota and improve mental well-being and gut symptoms, but there is currently a lack of evidence fo...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Litai, Poveda, Carlos, Jenkins, Paul E., Walton, Gemma E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703691/
https://www.ncbi.nlm.nih.gov/pubmed/34959997
http://dx.doi.org/10.3390/nu13124447
_version_ 1784621524867612672
author Liu, Litai
Poveda, Carlos
Jenkins, Paul E.
Walton, Gemma E.
author_facet Liu, Litai
Poveda, Carlos
Jenkins, Paul E.
Walton, Gemma E.
author_sort Liu, Litai
collection PubMed
description Individuals with anorexia nervosa (AN) often suffer psychological and gastrointestinal problems consistent with a dysregulated gut microbial community. Psychobiotics have been postulated to modify microbiota and improve mental well-being and gut symptoms, but there is currently a lack of evidence for such approaches in AN. The aim of this study was to use an in vitro colonic model to evaluate the impact of dietary restrictions associated with AN on the intestinal ecosystem and to assess the impact of pre and probiotic intervention. Bacteriology was quantified using flow cytometry combined with fluorescence in situ hybridisation and metabolic end products (including neurotransmitters) by gas chromatography and liquid chromatography mass spectrometry Consistent with previous research, the nutritional changes significantly reduced total microbiota and metabolites compared with healthy conditions. Pre and probiotic supplementation on restricted conditions enhanced the microbial community and modulated metabolic activity to resemble that of a healthy diet. The model system indicates that nutritional changes associated with AN can impact the microbial community, and that these changes can, at least in part, be restored through the use of pre and probiotic interventions.
format Online
Article
Text
id pubmed-8703691
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87036912021-12-25 An In Vitro Approach to Studying the Microbial Community and Impact of Pre and Probiotics under Anorexia Nervosa Related Dietary Restrictions Liu, Litai Poveda, Carlos Jenkins, Paul E. Walton, Gemma E. Nutrients Article Individuals with anorexia nervosa (AN) often suffer psychological and gastrointestinal problems consistent with a dysregulated gut microbial community. Psychobiotics have been postulated to modify microbiota and improve mental well-being and gut symptoms, but there is currently a lack of evidence for such approaches in AN. The aim of this study was to use an in vitro colonic model to evaluate the impact of dietary restrictions associated with AN on the intestinal ecosystem and to assess the impact of pre and probiotic intervention. Bacteriology was quantified using flow cytometry combined with fluorescence in situ hybridisation and metabolic end products (including neurotransmitters) by gas chromatography and liquid chromatography mass spectrometry Consistent with previous research, the nutritional changes significantly reduced total microbiota and metabolites compared with healthy conditions. Pre and probiotic supplementation on restricted conditions enhanced the microbial community and modulated metabolic activity to resemble that of a healthy diet. The model system indicates that nutritional changes associated with AN can impact the microbial community, and that these changes can, at least in part, be restored through the use of pre and probiotic interventions. MDPI 2021-12-13 /pmc/articles/PMC8703691/ /pubmed/34959997 http://dx.doi.org/10.3390/nu13124447 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Litai
Poveda, Carlos
Jenkins, Paul E.
Walton, Gemma E.
An In Vitro Approach to Studying the Microbial Community and Impact of Pre and Probiotics under Anorexia Nervosa Related Dietary Restrictions
title An In Vitro Approach to Studying the Microbial Community and Impact of Pre and Probiotics under Anorexia Nervosa Related Dietary Restrictions
title_full An In Vitro Approach to Studying the Microbial Community and Impact of Pre and Probiotics under Anorexia Nervosa Related Dietary Restrictions
title_fullStr An In Vitro Approach to Studying the Microbial Community and Impact of Pre and Probiotics under Anorexia Nervosa Related Dietary Restrictions
title_full_unstemmed An In Vitro Approach to Studying the Microbial Community and Impact of Pre and Probiotics under Anorexia Nervosa Related Dietary Restrictions
title_short An In Vitro Approach to Studying the Microbial Community and Impact of Pre and Probiotics under Anorexia Nervosa Related Dietary Restrictions
title_sort in vitro approach to studying the microbial community and impact of pre and probiotics under anorexia nervosa related dietary restrictions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703691/
https://www.ncbi.nlm.nih.gov/pubmed/34959997
http://dx.doi.org/10.3390/nu13124447
work_keys_str_mv AT liulitai aninvitroapproachtostudyingthemicrobialcommunityandimpactofpreandprobioticsunderanorexianervosarelateddietaryrestrictions
AT povedacarlos aninvitroapproachtostudyingthemicrobialcommunityandimpactofpreandprobioticsunderanorexianervosarelateddietaryrestrictions
AT jenkinspaule aninvitroapproachtostudyingthemicrobialcommunityandimpactofpreandprobioticsunderanorexianervosarelateddietaryrestrictions
AT waltongemmae aninvitroapproachtostudyingthemicrobialcommunityandimpactofpreandprobioticsunderanorexianervosarelateddietaryrestrictions
AT liulitai invitroapproachtostudyingthemicrobialcommunityandimpactofpreandprobioticsunderanorexianervosarelateddietaryrestrictions
AT povedacarlos invitroapproachtostudyingthemicrobialcommunityandimpactofpreandprobioticsunderanorexianervosarelateddietaryrestrictions
AT jenkinspaule invitroapproachtostudyingthemicrobialcommunityandimpactofpreandprobioticsunderanorexianervosarelateddietaryrestrictions
AT waltongemmae invitroapproachtostudyingthemicrobialcommunityandimpactofpreandprobioticsunderanorexianervosarelateddietaryrestrictions