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A Deep Learning Analysis Reveals Nitrogen-Doped Graphene Quantum Dots Damage Neurons of Nematode Caenorhabditis elegans

Along with the rapidly increasing applications of nitrogen-doped graphene quantum dots (N-GQDs) in the field of biomedicine, the exposure of N-GQDs undoubtedly pose a risk to the health of human beings, especially in the nervous system. In view of the lack of data from in vivo studies, this study us...

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Autores principales: Xu, Hongsheng, Wang, Xinyu, Zhang, Xiaomeng, Cheng, Jin, Zhang, Jixiang, Chen, Min, Wu, Tianshu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703693/
https://www.ncbi.nlm.nih.gov/pubmed/34947663
http://dx.doi.org/10.3390/nano11123314
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author Xu, Hongsheng
Wang, Xinyu
Zhang, Xiaomeng
Cheng, Jin
Zhang, Jixiang
Chen, Min
Wu, Tianshu
author_facet Xu, Hongsheng
Wang, Xinyu
Zhang, Xiaomeng
Cheng, Jin
Zhang, Jixiang
Chen, Min
Wu, Tianshu
author_sort Xu, Hongsheng
collection PubMed
description Along with the rapidly increasing applications of nitrogen-doped graphene quantum dots (N-GQDs) in the field of biomedicine, the exposure of N-GQDs undoubtedly pose a risk to the health of human beings, especially in the nervous system. In view of the lack of data from in vivo studies, this study used the nematode Caenorhabditis elegans (C. elegans), which has become a valuable animal model in nanotoxicological studies due to its multiple advantages, to undertake a bio-safety assessment of N-GQDs in the nervous system with the assistance of a deep learning model. The findings suggested that accumulated N-GQDs in the nematodes’ bodies damaged their normal behavior in a dose- and time-dependent manner, and the impairments of the nervous system were obviously severe when the exposure dosages were above 100 μg/mL. When assessing the morphological changes of neurons caused by N-GQDs, a quantitative image-based analysis based on a deep neural network algorithm (YOLACT) was used because traditional image-based analysis is labor-intensive and limited to qualitative evaluation. The quantitative results indicated that N-GQDs damaged dopaminergic and glutamatergic neurons, which are involved in the neurotoxic effects of N-GQDs in the nematode C. elegans. This study not only suggests a fast and economic C. elegans model to undertake the risk assessment of nanomaterials in the nervous system, but also provides a valuable deep learning approach to quantitatively track subtle morphological changes of neurons at an unbiased level in a nanotoxicological study using C. elegans.
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spelling pubmed-87036932021-12-25 A Deep Learning Analysis Reveals Nitrogen-Doped Graphene Quantum Dots Damage Neurons of Nematode Caenorhabditis elegans Xu, Hongsheng Wang, Xinyu Zhang, Xiaomeng Cheng, Jin Zhang, Jixiang Chen, Min Wu, Tianshu Nanomaterials (Basel) Article Along with the rapidly increasing applications of nitrogen-doped graphene quantum dots (N-GQDs) in the field of biomedicine, the exposure of N-GQDs undoubtedly pose a risk to the health of human beings, especially in the nervous system. In view of the lack of data from in vivo studies, this study used the nematode Caenorhabditis elegans (C. elegans), which has become a valuable animal model in nanotoxicological studies due to its multiple advantages, to undertake a bio-safety assessment of N-GQDs in the nervous system with the assistance of a deep learning model. The findings suggested that accumulated N-GQDs in the nematodes’ bodies damaged their normal behavior in a dose- and time-dependent manner, and the impairments of the nervous system were obviously severe when the exposure dosages were above 100 μg/mL. When assessing the morphological changes of neurons caused by N-GQDs, a quantitative image-based analysis based on a deep neural network algorithm (YOLACT) was used because traditional image-based analysis is labor-intensive and limited to qualitative evaluation. The quantitative results indicated that N-GQDs damaged dopaminergic and glutamatergic neurons, which are involved in the neurotoxic effects of N-GQDs in the nematode C. elegans. This study not only suggests a fast and economic C. elegans model to undertake the risk assessment of nanomaterials in the nervous system, but also provides a valuable deep learning approach to quantitatively track subtle morphological changes of neurons at an unbiased level in a nanotoxicological study using C. elegans. MDPI 2021-12-07 /pmc/articles/PMC8703693/ /pubmed/34947663 http://dx.doi.org/10.3390/nano11123314 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Hongsheng
Wang, Xinyu
Zhang, Xiaomeng
Cheng, Jin
Zhang, Jixiang
Chen, Min
Wu, Tianshu
A Deep Learning Analysis Reveals Nitrogen-Doped Graphene Quantum Dots Damage Neurons of Nematode Caenorhabditis elegans
title A Deep Learning Analysis Reveals Nitrogen-Doped Graphene Quantum Dots Damage Neurons of Nematode Caenorhabditis elegans
title_full A Deep Learning Analysis Reveals Nitrogen-Doped Graphene Quantum Dots Damage Neurons of Nematode Caenorhabditis elegans
title_fullStr A Deep Learning Analysis Reveals Nitrogen-Doped Graphene Quantum Dots Damage Neurons of Nematode Caenorhabditis elegans
title_full_unstemmed A Deep Learning Analysis Reveals Nitrogen-Doped Graphene Quantum Dots Damage Neurons of Nematode Caenorhabditis elegans
title_short A Deep Learning Analysis Reveals Nitrogen-Doped Graphene Quantum Dots Damage Neurons of Nematode Caenorhabditis elegans
title_sort deep learning analysis reveals nitrogen-doped graphene quantum dots damage neurons of nematode caenorhabditis elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703693/
https://www.ncbi.nlm.nih.gov/pubmed/34947663
http://dx.doi.org/10.3390/nano11123314
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