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Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress

Poplars can be harmed by poplar canker. Inoculation with mycorrhizal fungi can improve the resistance of poplars to canker, but the molecular mechanism is still unclear. In this study, an aseptic inoculation system of L. bicolor–P. trichocarpa–B. dothidea was constructed, and transcriptome analysis...

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Autores principales: Dong, Fengxin, Wang, Yihan, Tang, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703858/
https://www.ncbi.nlm.nih.gov/pubmed/34947006
http://dx.doi.org/10.3390/jof7121024
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author Dong, Fengxin
Wang, Yihan
Tang, Ming
author_facet Dong, Fengxin
Wang, Yihan
Tang, Ming
author_sort Dong, Fengxin
collection PubMed
description Poplars can be harmed by poplar canker. Inoculation with mycorrhizal fungi can improve the resistance of poplars to canker, but the molecular mechanism is still unclear. In this study, an aseptic inoculation system of L. bicolor–P. trichocarpa–B. dothidea was constructed, and transcriptome analysis was performed to investigate regulation by L. bicolor of the expression of genes in the roots of P. trichocarpa during the onset of B. dothidea infection, and a total of 3022 differentially expressed genes (DEGs) were identified. Weighted correlation network analysis (WGCNA) was performed on these DEGs, and 661 genes’ expressions were considered to be affected by inoculation with L. bicolor and B. dothidea. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that these 661 DEGs were involved in multiple pathways such as signal transduction, reactive oxygen metabolism, and plant-pathogen interaction. Inoculation with L. bicolor changed the gene expression pattern of the roots, evidencing its involvement in the disease resistance response of P. trichocarpa. This research reveals the mechanism of L. bicolor in inducing resistance to canker of P. trichocarpa at the molecular level and provides a theoretical basis for the practical application of mycorrhizal fungi to improve plant disease resistance.
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spelling pubmed-87038582021-12-25 Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress Dong, Fengxin Wang, Yihan Tang, Ming J Fungi (Basel) Article Poplars can be harmed by poplar canker. Inoculation with mycorrhizal fungi can improve the resistance of poplars to canker, but the molecular mechanism is still unclear. In this study, an aseptic inoculation system of L. bicolor–P. trichocarpa–B. dothidea was constructed, and transcriptome analysis was performed to investigate regulation by L. bicolor of the expression of genes in the roots of P. trichocarpa during the onset of B. dothidea infection, and a total of 3022 differentially expressed genes (DEGs) were identified. Weighted correlation network analysis (WGCNA) was performed on these DEGs, and 661 genes’ expressions were considered to be affected by inoculation with L. bicolor and B. dothidea. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that these 661 DEGs were involved in multiple pathways such as signal transduction, reactive oxygen metabolism, and plant-pathogen interaction. Inoculation with L. bicolor changed the gene expression pattern of the roots, evidencing its involvement in the disease resistance response of P. trichocarpa. This research reveals the mechanism of L. bicolor in inducing resistance to canker of P. trichocarpa at the molecular level and provides a theoretical basis for the practical application of mycorrhizal fungi to improve plant disease resistance. MDPI 2021-11-29 /pmc/articles/PMC8703858/ /pubmed/34947006 http://dx.doi.org/10.3390/jof7121024 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dong, Fengxin
Wang, Yihan
Tang, Ming
Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress
title Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress
title_full Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress
title_fullStr Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress
title_full_unstemmed Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress
title_short Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress
title_sort effects of laccaria bicolor on gene expression of populus trichocarpa root under poplar canker stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703858/
https://www.ncbi.nlm.nih.gov/pubmed/34947006
http://dx.doi.org/10.3390/jof7121024
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