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The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach
Malignant mesothelioma (MM) is an aggressive asbestos-related cancer, against which no curative modalities exist. Oncolytic virotherapy is a promising therapeutic approach, for which MM is an ideal candidate; indeed, the pleural location provides direct access for the intra-tumoral injection of onco...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703924/ https://www.ncbi.nlm.nih.gov/pubmed/34960727 http://dx.doi.org/10.3390/v13122458 |
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author | Forte, Iris Maria Indovina, Paola Montagnaro, Serena Costa, Aurora Iannuzzi, Carmelina Antonella Capone, Francesca Camerlingo, Rosa Malfitano, Anna Maria Pentimalli, Francesca Ferrara, Gianmarco Quintiliani, Massimiliamo Portella, Giuseppe Giordano, Antonio Ciarcia, Roberto |
author_facet | Forte, Iris Maria Indovina, Paola Montagnaro, Serena Costa, Aurora Iannuzzi, Carmelina Antonella Capone, Francesca Camerlingo, Rosa Malfitano, Anna Maria Pentimalli, Francesca Ferrara, Gianmarco Quintiliani, Massimiliamo Portella, Giuseppe Giordano, Antonio Ciarcia, Roberto |
author_sort | Forte, Iris Maria |
collection | PubMed |
description | Malignant mesothelioma (MM) is an aggressive asbestos-related cancer, against which no curative modalities exist. Oncolytic virotherapy is a promising therapeutic approach, for which MM is an ideal candidate; indeed, the pleural location provides direct access for the intra-tumoral injection of oncolytic viruses (OVs). Some non-human OVs offer advantages over human OVs, including the non-pathogenicity in humans and the absence of pre-existing immunity. We previously showed that caprine herpesvirus 1 (CpHV-1), a non-pathogenic virus for humans, can kill different human cancer cell lines. Here, we assessed CpHV-1 effects on MM (NCI-H28, MSTO, NCI-H2052) and non-tumor mesothelial (MET-5A) cells. We found that CpHV-1 reduced cell viability and clonogenic potential in all MM cell lines without affecting non-tumor cells, in which, indeed, we did not detect intracellular viral DNA after treatment. In particular, CpHV-1 induced MM cell apoptosis and accumulation in G0/G1 or S cell cycle phases. Moreover, CpHV-1 strongly synergized with cisplatin, the drug currently used in MM chemotherapy, and this agent combination did not affect normal mesothelial cells. Although further studies are required to elucidate the mechanisms underlying the selective CpHV-1 action on MM cells, our data suggest that the CpHV-1-cisplatin combination could be a feasible strategy against MM. |
format | Online Article Text |
id | pubmed-8703924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87039242021-12-25 The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach Forte, Iris Maria Indovina, Paola Montagnaro, Serena Costa, Aurora Iannuzzi, Carmelina Antonella Capone, Francesca Camerlingo, Rosa Malfitano, Anna Maria Pentimalli, Francesca Ferrara, Gianmarco Quintiliani, Massimiliamo Portella, Giuseppe Giordano, Antonio Ciarcia, Roberto Viruses Article Malignant mesothelioma (MM) is an aggressive asbestos-related cancer, against which no curative modalities exist. Oncolytic virotherapy is a promising therapeutic approach, for which MM is an ideal candidate; indeed, the pleural location provides direct access for the intra-tumoral injection of oncolytic viruses (OVs). Some non-human OVs offer advantages over human OVs, including the non-pathogenicity in humans and the absence of pre-existing immunity. We previously showed that caprine herpesvirus 1 (CpHV-1), a non-pathogenic virus for humans, can kill different human cancer cell lines. Here, we assessed CpHV-1 effects on MM (NCI-H28, MSTO, NCI-H2052) and non-tumor mesothelial (MET-5A) cells. We found that CpHV-1 reduced cell viability and clonogenic potential in all MM cell lines without affecting non-tumor cells, in which, indeed, we did not detect intracellular viral DNA after treatment. In particular, CpHV-1 induced MM cell apoptosis and accumulation in G0/G1 or S cell cycle phases. Moreover, CpHV-1 strongly synergized with cisplatin, the drug currently used in MM chemotherapy, and this agent combination did not affect normal mesothelial cells. Although further studies are required to elucidate the mechanisms underlying the selective CpHV-1 action on MM cells, our data suggest that the CpHV-1-cisplatin combination could be a feasible strategy against MM. MDPI 2021-12-08 /pmc/articles/PMC8703924/ /pubmed/34960727 http://dx.doi.org/10.3390/v13122458 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Forte, Iris Maria Indovina, Paola Montagnaro, Serena Costa, Aurora Iannuzzi, Carmelina Antonella Capone, Francesca Camerlingo, Rosa Malfitano, Anna Maria Pentimalli, Francesca Ferrara, Gianmarco Quintiliani, Massimiliamo Portella, Giuseppe Giordano, Antonio Ciarcia, Roberto The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach |
title | The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach |
title_full | The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach |
title_fullStr | The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach |
title_full_unstemmed | The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach |
title_short | The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach |
title_sort | oncolytic caprine herpesvirus 1 (cphv-1) induces apoptosis and synergizes with cisplatin in mesothelioma cell lines: a new potential virotherapy approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703924/ https://www.ncbi.nlm.nih.gov/pubmed/34960727 http://dx.doi.org/10.3390/v13122458 |
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