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The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach

Malignant mesothelioma (MM) is an aggressive asbestos-related cancer, against which no curative modalities exist. Oncolytic virotherapy is a promising therapeutic approach, for which MM is an ideal candidate; indeed, the pleural location provides direct access for the intra-tumoral injection of onco...

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Autores principales: Forte, Iris Maria, Indovina, Paola, Montagnaro, Serena, Costa, Aurora, Iannuzzi, Carmelina Antonella, Capone, Francesca, Camerlingo, Rosa, Malfitano, Anna Maria, Pentimalli, Francesca, Ferrara, Gianmarco, Quintiliani, Massimiliamo, Portella, Giuseppe, Giordano, Antonio, Ciarcia, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703924/
https://www.ncbi.nlm.nih.gov/pubmed/34960727
http://dx.doi.org/10.3390/v13122458
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author Forte, Iris Maria
Indovina, Paola
Montagnaro, Serena
Costa, Aurora
Iannuzzi, Carmelina Antonella
Capone, Francesca
Camerlingo, Rosa
Malfitano, Anna Maria
Pentimalli, Francesca
Ferrara, Gianmarco
Quintiliani, Massimiliamo
Portella, Giuseppe
Giordano, Antonio
Ciarcia, Roberto
author_facet Forte, Iris Maria
Indovina, Paola
Montagnaro, Serena
Costa, Aurora
Iannuzzi, Carmelina Antonella
Capone, Francesca
Camerlingo, Rosa
Malfitano, Anna Maria
Pentimalli, Francesca
Ferrara, Gianmarco
Quintiliani, Massimiliamo
Portella, Giuseppe
Giordano, Antonio
Ciarcia, Roberto
author_sort Forte, Iris Maria
collection PubMed
description Malignant mesothelioma (MM) is an aggressive asbestos-related cancer, against which no curative modalities exist. Oncolytic virotherapy is a promising therapeutic approach, for which MM is an ideal candidate; indeed, the pleural location provides direct access for the intra-tumoral injection of oncolytic viruses (OVs). Some non-human OVs offer advantages over human OVs, including the non-pathogenicity in humans and the absence of pre-existing immunity. We previously showed that caprine herpesvirus 1 (CpHV-1), a non-pathogenic virus for humans, can kill different human cancer cell lines. Here, we assessed CpHV-1 effects on MM (NCI-H28, MSTO, NCI-H2052) and non-tumor mesothelial (MET-5A) cells. We found that CpHV-1 reduced cell viability and clonogenic potential in all MM cell lines without affecting non-tumor cells, in which, indeed, we did not detect intracellular viral DNA after treatment. In particular, CpHV-1 induced MM cell apoptosis and accumulation in G0/G1 or S cell cycle phases. Moreover, CpHV-1 strongly synergized with cisplatin, the drug currently used in MM chemotherapy, and this agent combination did not affect normal mesothelial cells. Although further studies are required to elucidate the mechanisms underlying the selective CpHV-1 action on MM cells, our data suggest that the CpHV-1-cisplatin combination could be a feasible strategy against MM.
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spelling pubmed-87039242021-12-25 The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach Forte, Iris Maria Indovina, Paola Montagnaro, Serena Costa, Aurora Iannuzzi, Carmelina Antonella Capone, Francesca Camerlingo, Rosa Malfitano, Anna Maria Pentimalli, Francesca Ferrara, Gianmarco Quintiliani, Massimiliamo Portella, Giuseppe Giordano, Antonio Ciarcia, Roberto Viruses Article Malignant mesothelioma (MM) is an aggressive asbestos-related cancer, against which no curative modalities exist. Oncolytic virotherapy is a promising therapeutic approach, for which MM is an ideal candidate; indeed, the pleural location provides direct access for the intra-tumoral injection of oncolytic viruses (OVs). Some non-human OVs offer advantages over human OVs, including the non-pathogenicity in humans and the absence of pre-existing immunity. We previously showed that caprine herpesvirus 1 (CpHV-1), a non-pathogenic virus for humans, can kill different human cancer cell lines. Here, we assessed CpHV-1 effects on MM (NCI-H28, MSTO, NCI-H2052) and non-tumor mesothelial (MET-5A) cells. We found that CpHV-1 reduced cell viability and clonogenic potential in all MM cell lines without affecting non-tumor cells, in which, indeed, we did not detect intracellular viral DNA after treatment. In particular, CpHV-1 induced MM cell apoptosis and accumulation in G0/G1 or S cell cycle phases. Moreover, CpHV-1 strongly synergized with cisplatin, the drug currently used in MM chemotherapy, and this agent combination did not affect normal mesothelial cells. Although further studies are required to elucidate the mechanisms underlying the selective CpHV-1 action on MM cells, our data suggest that the CpHV-1-cisplatin combination could be a feasible strategy against MM. MDPI 2021-12-08 /pmc/articles/PMC8703924/ /pubmed/34960727 http://dx.doi.org/10.3390/v13122458 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Forte, Iris Maria
Indovina, Paola
Montagnaro, Serena
Costa, Aurora
Iannuzzi, Carmelina Antonella
Capone, Francesca
Camerlingo, Rosa
Malfitano, Anna Maria
Pentimalli, Francesca
Ferrara, Gianmarco
Quintiliani, Massimiliamo
Portella, Giuseppe
Giordano, Antonio
Ciarcia, Roberto
The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach
title The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach
title_full The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach
title_fullStr The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach
title_full_unstemmed The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach
title_short The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach
title_sort oncolytic caprine herpesvirus 1 (cphv-1) induces apoptosis and synergizes with cisplatin in mesothelioma cell lines: a new potential virotherapy approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703924/
https://www.ncbi.nlm.nih.gov/pubmed/34960727
http://dx.doi.org/10.3390/v13122458
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