Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots

Neonatal dried blood spots (DBS) provide a remarkable resource for biobanks. These microsamples can provide information related to the genetic correlates of disease and can be used to quantify a range of analytes, such as proteins and small molecules. However, after routine neonatal screening, the a...

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Detalles Bibliográficos
Autores principales: Boelt, Sanne Grundvad, Melgaard, Lars, Thorbek, Marta Jadwiga, MacSween, Nadia Sara Jensen, McGrath, John J., Cohen, Arieh S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704356/
https://www.ncbi.nlm.nih.gov/pubmed/34940052
http://dx.doi.org/10.3390/ijns7040082
Descripción
Sumario:Neonatal dried blood spots (DBS) provide a remarkable resource for biobanks. These microsamples can provide information related to the genetic correlates of disease and can be used to quantify a range of analytes, such as proteins and small molecules. However, after routine neonatal screening, the amount of DBS sample available is limited. To optimize the use of these samples, there is a need for sensitive assays which are integrated across different analytic platforms. For example, after DNA extraction, protein extracts are available for additional analyses. We describe a sensitive and robust LC-MS/MS method for 25-hydroxyvitamin D(2) and 25-hydroxyvitamin D(3) optimized for leftover protein extracts from DBS, which has excellent recovery, precision, and accuracy.

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