Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots

Neonatal dried blood spots (DBS) provide a remarkable resource for biobanks. These microsamples can provide information related to the genetic correlates of disease and can be used to quantify a range of analytes, such as proteins and small molecules. However, after routine neonatal screening, the a...

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Autores principales: Boelt, Sanne Grundvad, Melgaard, Lars, Thorbek, Marta Jadwiga, MacSween, Nadia Sara Jensen, McGrath, John J., Cohen, Arieh S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704356/
https://www.ncbi.nlm.nih.gov/pubmed/34940052
http://dx.doi.org/10.3390/ijns7040082
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author Boelt, Sanne Grundvad
Melgaard, Lars
Thorbek, Marta Jadwiga
MacSween, Nadia Sara Jensen
McGrath, John J.
Cohen, Arieh S.
author_facet Boelt, Sanne Grundvad
Melgaard, Lars
Thorbek, Marta Jadwiga
MacSween, Nadia Sara Jensen
McGrath, John J.
Cohen, Arieh S.
author_sort Boelt, Sanne Grundvad
collection PubMed
description Neonatal dried blood spots (DBS) provide a remarkable resource for biobanks. These microsamples can provide information related to the genetic correlates of disease and can be used to quantify a range of analytes, such as proteins and small molecules. However, after routine neonatal screening, the amount of DBS sample available is limited. To optimize the use of these samples, there is a need for sensitive assays which are integrated across different analytic platforms. For example, after DNA extraction, protein extracts are available for additional analyses. We describe a sensitive and robust LC-MS/MS method for 25-hydroxyvitamin D(2) and 25-hydroxyvitamin D(3) optimized for leftover protein extracts from DBS, which has excellent recovery, precision, and accuracy.
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spelling pubmed-87043562021-12-25 Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots Boelt, Sanne Grundvad Melgaard, Lars Thorbek, Marta Jadwiga MacSween, Nadia Sara Jensen McGrath, John J. Cohen, Arieh S. Int J Neonatal Screen Technical Note Neonatal dried blood spots (DBS) provide a remarkable resource for biobanks. These microsamples can provide information related to the genetic correlates of disease and can be used to quantify a range of analytes, such as proteins and small molecules. However, after routine neonatal screening, the amount of DBS sample available is limited. To optimize the use of these samples, there is a need for sensitive assays which are integrated across different analytic platforms. For example, after DNA extraction, protein extracts are available for additional analyses. We describe a sensitive and robust LC-MS/MS method for 25-hydroxyvitamin D(2) and 25-hydroxyvitamin D(3) optimized for leftover protein extracts from DBS, which has excellent recovery, precision, and accuracy. MDPI 2021-12-10 /pmc/articles/PMC8704356/ /pubmed/34940052 http://dx.doi.org/10.3390/ijns7040082 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Boelt, Sanne Grundvad
Melgaard, Lars
Thorbek, Marta Jadwiga
MacSween, Nadia Sara Jensen
McGrath, John J.
Cohen, Arieh S.
Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots
title Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots
title_full Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots
title_fullStr Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots
title_full_unstemmed Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots
title_short Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots
title_sort sensitive and robust lc-ms/ms assay to quantify 25-hydroxyvitamin d in leftover protein extract from dried blood spots
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704356/
https://www.ncbi.nlm.nih.gov/pubmed/34940052
http://dx.doi.org/10.3390/ijns7040082
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