Cargando…

Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells

A core challenge in the field of tissue engineering is the ability to establish pipeline workflows for the design and characterization of scaffold technologies with clinically translatable attributes. The parallel development of biomaterials and stem cell populations represents a self-sufficient and...

Descripción completa

Detalles Bibliográficos
Autores principales: Bow, Austin J., Masi, Thomas J., Dhar, Madhu S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704510/
https://www.ncbi.nlm.nih.gov/pubmed/34959426
http://dx.doi.org/10.3390/pharmaceutics13122146
_version_ 1784621723872657408
author Bow, Austin J.
Masi, Thomas J.
Dhar, Madhu S.
author_facet Bow, Austin J.
Masi, Thomas J.
Dhar, Madhu S.
author_sort Bow, Austin J.
collection PubMed
description A core challenge in the field of tissue engineering is the ability to establish pipeline workflows for the design and characterization of scaffold technologies with clinically translatable attributes. The parallel development of biomaterials and stem cell populations represents a self-sufficient and streamlined approach for establishing such a pipeline. In the current study, rat dental pulp stem cell (rDPSC) populations were established to assess functionalized polycaprolactone (PCL) constructs. Initial optimization and characterization of rDPSC extraction and culture conditions confirmed that cell populations were readily expandable and demonstrated surface markers associated with multi-potency. Subset populations were transduced to express DsRed fluorescent protein as a mechanism of tracking both cells and cell-derived extracellular matrix content on complex scaffold architecture. Thermoplastic constructs included reduced graphene oxide (rGO) as an additive to promote cellular attachment and were further modified by surface etching a weak acetic acid solution to roughen surface topographical features, which was observed to dramatically improve cell surface coverage in vitro. Based on these data, the modified rGO-functionalized PCL constructs represent a versatile platform for bone tissue engineering, capable of being applied as a standalone matrix or in conjunction with bio-active payloads such as DPSCs or other bio-inks.
format Online
Article
Text
id pubmed-8704510
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87045102021-12-25 Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells Bow, Austin J. Masi, Thomas J. Dhar, Madhu S. Pharmaceutics Article A core challenge in the field of tissue engineering is the ability to establish pipeline workflows for the design and characterization of scaffold technologies with clinically translatable attributes. The parallel development of biomaterials and stem cell populations represents a self-sufficient and streamlined approach for establishing such a pipeline. In the current study, rat dental pulp stem cell (rDPSC) populations were established to assess functionalized polycaprolactone (PCL) constructs. Initial optimization and characterization of rDPSC extraction and culture conditions confirmed that cell populations were readily expandable and demonstrated surface markers associated with multi-potency. Subset populations were transduced to express DsRed fluorescent protein as a mechanism of tracking both cells and cell-derived extracellular matrix content on complex scaffold architecture. Thermoplastic constructs included reduced graphene oxide (rGO) as an additive to promote cellular attachment and were further modified by surface etching a weak acetic acid solution to roughen surface topographical features, which was observed to dramatically improve cell surface coverage in vitro. Based on these data, the modified rGO-functionalized PCL constructs represent a versatile platform for bone tissue engineering, capable of being applied as a standalone matrix or in conjunction with bio-active payloads such as DPSCs or other bio-inks. MDPI 2021-12-13 /pmc/articles/PMC8704510/ /pubmed/34959426 http://dx.doi.org/10.3390/pharmaceutics13122146 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bow, Austin J.
Masi, Thomas J.
Dhar, Madhu S.
Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells
title Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells
title_full Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells
title_fullStr Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells
title_full_unstemmed Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells
title_short Etched 3D-Printed Polycaprolactone Constructs Functionalized with Reduced Graphene Oxide for Enhanced Attachment of Dental Pulp-Derived Stem Cells
title_sort etched 3d-printed polycaprolactone constructs functionalized with reduced graphene oxide for enhanced attachment of dental pulp-derived stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704510/
https://www.ncbi.nlm.nih.gov/pubmed/34959426
http://dx.doi.org/10.3390/pharmaceutics13122146
work_keys_str_mv AT bowaustinj etched3dprintedpolycaprolactoneconstructsfunctionalizedwithreducedgrapheneoxideforenhancedattachmentofdentalpulpderivedstemcells
AT masithomasj etched3dprintedpolycaprolactoneconstructsfunctionalizedwithreducedgrapheneoxideforenhancedattachmentofdentalpulpderivedstemcells
AT dharmadhus etched3dprintedpolycaprolactoneconstructsfunctionalizedwithreducedgrapheneoxideforenhancedattachmentofdentalpulpderivedstemcells