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Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation

Dogroses belong to a taxonomically difficult genus and family and represent important phytogenetic resources associated with high ornamental, pharmaceutical-cosmetic and nutritional values, thus suggesting a potentially high exploitation merit. Triggered by these prospects, wild-growing Rosa canina...

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Autores principales: Maloupa, Eleni, Karapatzak, Eleftherios, Ganopoulos, Ioannis, Karydas, Antonis, Papanastasi, Katerina, Kyrkas, Dimitris, Yfanti, Paraskevi, Nikisianis, Nikos, Zahariadis, Anthimos, Kosma, Ioanna S., Badeka, Anastasia V., Patakioutas, Giorgos, Fotakis, Dimitrios, Krigas, Nikos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704562/
https://www.ncbi.nlm.nih.gov/pubmed/34961105
http://dx.doi.org/10.3390/plants10122634
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author Maloupa, Eleni
Karapatzak, Eleftherios
Ganopoulos, Ioannis
Karydas, Antonis
Papanastasi, Katerina
Kyrkas, Dimitris
Yfanti, Paraskevi
Nikisianis, Nikos
Zahariadis, Anthimos
Kosma, Ioanna S.
Badeka, Anastasia V.
Patakioutas, Giorgos
Fotakis, Dimitrios
Krigas, Nikos
author_facet Maloupa, Eleni
Karapatzak, Eleftherios
Ganopoulos, Ioannis
Karydas, Antonis
Papanastasi, Katerina
Kyrkas, Dimitris
Yfanti, Paraskevi
Nikisianis, Nikos
Zahariadis, Anthimos
Kosma, Ioanna S.
Badeka, Anastasia V.
Patakioutas, Giorgos
Fotakis, Dimitrios
Krigas, Nikos
author_sort Maloupa, Eleni
collection PubMed
description Dogroses belong to a taxonomically difficult genus and family and represent important phytogenetic resources associated with high ornamental, pharmaceutical-cosmetic and nutritional values, thus suggesting a potentially high exploitation merit. Triggered by these prospects, wild-growing Rosa canina populations of Greece were selected for investigation and evaluation of their potential for integrated domestication. We collected ripe rosehips from Greek native wild-growing populations (samples from seven genotypes) for phytochemical analysis (total phenolics, total flavonoids, antioxidant activity and vitamin C content), leaf samples for DNA analysis using the ITS2 sequence (nine genotypes) and fresh soft-wood stem cuttings for propagation trials (seven genotypes). After evaluation of these materials, this study reports for the first-time distinct DNA-fingerprinted genotypes from Greece with interesting phytochemical profiles mainly in terms of Vitamic C content (up to 500.22 ± 0.15 mg of ascorbic acid equivalents/100 g of sample) as well as effective asexual propagation protocols for prioritized R. canina genotypes via cuttings. The latter highlights the importance of the levels of external hormone application (2000 ppm of indole-3-butyric acid), the effect of season (highly-effective spring trials) and genotype-specific differences in rooting capacities of the studied genotypes. All inclusive, this study offers new artificially selected material of Greek native R. canina with a consolidated identity and interesting phytochemical profile. These materials are currently under ex-situ conservation for further evaluation and characterization in pilot field studies, thus facilitating its sustainable exploitation for applications in the agro-alimentary, medicinal-cosmetic, and ornamental sectors.
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spelling pubmed-87045622021-12-25 Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation Maloupa, Eleni Karapatzak, Eleftherios Ganopoulos, Ioannis Karydas, Antonis Papanastasi, Katerina Kyrkas, Dimitris Yfanti, Paraskevi Nikisianis, Nikos Zahariadis, Anthimos Kosma, Ioanna S. Badeka, Anastasia V. Patakioutas, Giorgos Fotakis, Dimitrios Krigas, Nikos Plants (Basel) Article Dogroses belong to a taxonomically difficult genus and family and represent important phytogenetic resources associated with high ornamental, pharmaceutical-cosmetic and nutritional values, thus suggesting a potentially high exploitation merit. Triggered by these prospects, wild-growing Rosa canina populations of Greece were selected for investigation and evaluation of their potential for integrated domestication. We collected ripe rosehips from Greek native wild-growing populations (samples from seven genotypes) for phytochemical analysis (total phenolics, total flavonoids, antioxidant activity and vitamin C content), leaf samples for DNA analysis using the ITS2 sequence (nine genotypes) and fresh soft-wood stem cuttings for propagation trials (seven genotypes). After evaluation of these materials, this study reports for the first-time distinct DNA-fingerprinted genotypes from Greece with interesting phytochemical profiles mainly in terms of Vitamic C content (up to 500.22 ± 0.15 mg of ascorbic acid equivalents/100 g of sample) as well as effective asexual propagation protocols for prioritized R. canina genotypes via cuttings. The latter highlights the importance of the levels of external hormone application (2000 ppm of indole-3-butyric acid), the effect of season (highly-effective spring trials) and genotype-specific differences in rooting capacities of the studied genotypes. All inclusive, this study offers new artificially selected material of Greek native R. canina with a consolidated identity and interesting phytochemical profile. These materials are currently under ex-situ conservation for further evaluation and characterization in pilot field studies, thus facilitating its sustainable exploitation for applications in the agro-alimentary, medicinal-cosmetic, and ornamental sectors. MDPI 2021-11-30 /pmc/articles/PMC8704562/ /pubmed/34961105 http://dx.doi.org/10.3390/plants10122634 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maloupa, Eleni
Karapatzak, Eleftherios
Ganopoulos, Ioannis
Karydas, Antonis
Papanastasi, Katerina
Kyrkas, Dimitris
Yfanti, Paraskevi
Nikisianis, Nikos
Zahariadis, Anthimos
Kosma, Ioanna S.
Badeka, Anastasia V.
Patakioutas, Giorgos
Fotakis, Dimitrios
Krigas, Nikos
Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation
title Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation
title_full Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation
title_fullStr Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation
title_full_unstemmed Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation
title_short Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation
title_sort molecular authentication, phytochemical evaluation and asexual propagation of wild-growing rosa canina l. (rosaceae) genotypes of northern greece for sustainable exploitation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704562/
https://www.ncbi.nlm.nih.gov/pubmed/34961105
http://dx.doi.org/10.3390/plants10122634
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