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Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology
Permethrin (PERM) is a member of the class I family of synthetic pyrethroids. Human use has shown that it affects different systems, with wide health dysfunctions. Our aim was to determine bioenergetics, neuroinflammation and morphology changes, as redox markers after subacute exposure to PERM in ra...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704605/ https://www.ncbi.nlm.nih.gov/pubmed/34941771 http://dx.doi.org/10.3390/toxics9120337 |
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author | López-Aceves, Teresita Guadalupe Coballase-Urrutia, Elvia Estrada-Rojo, Francisco Vanoye-Carlo, América Carmona-Aparicio, Liliana Hernández, María Eugenia Pedraza-Chaverri, José Navarro, Luz Aparicio-Trejo, Omar E. Pérez-Torres, Armando Medina-Campos, Omar N. Martínez-Fong, Daniel Sánchez-Valle, Vicente Cárdenas-Rodríguez, Noemi Granados-Rojas, Leticia Pulido-Camarillo, Evelyn Rodríguez-Mata, Verónica León-Sicairos, Claudia del R. |
author_facet | López-Aceves, Teresita Guadalupe Coballase-Urrutia, Elvia Estrada-Rojo, Francisco Vanoye-Carlo, América Carmona-Aparicio, Liliana Hernández, María Eugenia Pedraza-Chaverri, José Navarro, Luz Aparicio-Trejo, Omar E. Pérez-Torres, Armando Medina-Campos, Omar N. Martínez-Fong, Daniel Sánchez-Valle, Vicente Cárdenas-Rodríguez, Noemi Granados-Rojas, Leticia Pulido-Camarillo, Evelyn Rodríguez-Mata, Verónica León-Sicairos, Claudia del R. |
author_sort | López-Aceves, Teresita Guadalupe |
collection | PubMed |
description | Permethrin (PERM) is a member of the class I family of synthetic pyrethroids. Human use has shown that it affects different systems, with wide health dysfunctions. Our aim was to determine bioenergetics, neuroinflammation and morphology changes, as redox markers after subacute exposure to PERM in rats. We used MDA determination, protein carbonyl assay, mitochondrial O(2) consumption, expression of pro-inflammatory cytokines and a deep histopathological analysis of the hippocampus. PERM (150 mg/kg and 300 mg/kg body weight/day, o.v.) increased lipoperoxidation and carbonylated proteins in a dose-dependent manner in the brain regions. The activities of antioxidant enzymes glutathione peroxidase, reductase, S-transferase, catalase, and superoxide dismutase showed an increase in all the different brain areas, with dose-dependent effects in the cerebellum. Cytokine profiles (IL-1β, IL-6 and TNF-α) increased in a dose-dependent manner in different brain tissues. Exposure to 150 mg/kg of permethrin induced degenerated and/or dead neurons in the rat hippocampus and induced mitochondrial uncoupling and reduction of oxidative phosphorylation and significantly decreased the respiratory parameters state 3-associated respiration in complex I and II. PERM exposure at low doses induces reactive oxygen species production and imbalance in the enzymatic antioxidant system, increases gene expression of pro-inflammatory interleukins, and could lead to cell damage mediated by mitochondrial functional impairment. |
format | Online Article Text |
id | pubmed-8704605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87046052021-12-25 Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology López-Aceves, Teresita Guadalupe Coballase-Urrutia, Elvia Estrada-Rojo, Francisco Vanoye-Carlo, América Carmona-Aparicio, Liliana Hernández, María Eugenia Pedraza-Chaverri, José Navarro, Luz Aparicio-Trejo, Omar E. Pérez-Torres, Armando Medina-Campos, Omar N. Martínez-Fong, Daniel Sánchez-Valle, Vicente Cárdenas-Rodríguez, Noemi Granados-Rojas, Leticia Pulido-Camarillo, Evelyn Rodríguez-Mata, Verónica León-Sicairos, Claudia del R. Toxics Article Permethrin (PERM) is a member of the class I family of synthetic pyrethroids. Human use has shown that it affects different systems, with wide health dysfunctions. Our aim was to determine bioenergetics, neuroinflammation and morphology changes, as redox markers after subacute exposure to PERM in rats. We used MDA determination, protein carbonyl assay, mitochondrial O(2) consumption, expression of pro-inflammatory cytokines and a deep histopathological analysis of the hippocampus. PERM (150 mg/kg and 300 mg/kg body weight/day, o.v.) increased lipoperoxidation and carbonylated proteins in a dose-dependent manner in the brain regions. The activities of antioxidant enzymes glutathione peroxidase, reductase, S-transferase, catalase, and superoxide dismutase showed an increase in all the different brain areas, with dose-dependent effects in the cerebellum. Cytokine profiles (IL-1β, IL-6 and TNF-α) increased in a dose-dependent manner in different brain tissues. Exposure to 150 mg/kg of permethrin induced degenerated and/or dead neurons in the rat hippocampus and induced mitochondrial uncoupling and reduction of oxidative phosphorylation and significantly decreased the respiratory parameters state 3-associated respiration in complex I and II. PERM exposure at low doses induces reactive oxygen species production and imbalance in the enzymatic antioxidant system, increases gene expression of pro-inflammatory interleukins, and could lead to cell damage mediated by mitochondrial functional impairment. MDPI 2021-12-06 /pmc/articles/PMC8704605/ /pubmed/34941771 http://dx.doi.org/10.3390/toxics9120337 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article López-Aceves, Teresita Guadalupe Coballase-Urrutia, Elvia Estrada-Rojo, Francisco Vanoye-Carlo, América Carmona-Aparicio, Liliana Hernández, María Eugenia Pedraza-Chaverri, José Navarro, Luz Aparicio-Trejo, Omar E. Pérez-Torres, Armando Medina-Campos, Omar N. Martínez-Fong, Daniel Sánchez-Valle, Vicente Cárdenas-Rodríguez, Noemi Granados-Rojas, Leticia Pulido-Camarillo, Evelyn Rodríguez-Mata, Verónica León-Sicairos, Claudia del R. Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
title | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
title_full | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
title_fullStr | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
title_full_unstemmed | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
title_short | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
title_sort | exposure to sub-lethal doses of permethrin is associated with neurotoxicity: changes in bioenergetics, redox markers, neuroinflammation and morphology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704605/ https://www.ncbi.nlm.nih.gov/pubmed/34941771 http://dx.doi.org/10.3390/toxics9120337 |
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