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Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018–2019

We performed whole genome sequencing and genetic characterization of rabies viruses (RABV) detected in bats submitted to the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) during 2018–2019. Among 88 bats submitted to CVMDL, six brain samples (6.8%, 95% confidence interval: 1.6% to 12.1...

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Autores principales: Hyeon, Ji-Yeon, Risatti, Guillermo R., Helal, Zeinab H., McGinnis, Holly, Sims, Maureen, Hunt, Amelia, Chung, David H., Kim, Junwon, Desiato, Julia, Lee, Dong-Hun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704678/
https://www.ncbi.nlm.nih.gov/pubmed/34960769
http://dx.doi.org/10.3390/v13122500
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author Hyeon, Ji-Yeon
Risatti, Guillermo R.
Helal, Zeinab H.
McGinnis, Holly
Sims, Maureen
Hunt, Amelia
Chung, David H.
Kim, Junwon
Desiato, Julia
Lee, Dong-Hun
author_facet Hyeon, Ji-Yeon
Risatti, Guillermo R.
Helal, Zeinab H.
McGinnis, Holly
Sims, Maureen
Hunt, Amelia
Chung, David H.
Kim, Junwon
Desiato, Julia
Lee, Dong-Hun
author_sort Hyeon, Ji-Yeon
collection PubMed
description We performed whole genome sequencing and genetic characterization of rabies viruses (RABV) detected in bats submitted to the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) during 2018–2019. Among 88 bats submitted to CVMDL, six brain samples (6.8%, 95% confidence interval: 1.6% to 12.1%) tested positive by direct fluorescent antibody test. RABVs were detected in big brown bats (Eptesicus fuscus, n = 4), a hoary bat (Lasiurus cinereus, n = 1), and an unidentified bat species (n = 1). Complete coding sequences of four out of six detected RABVs were obtained. In phylogenetic analysis, the RABVs (18-62, 18-4347, and 19-2274) from big brown bats belong to the bats EF-E1 clade, clustering with RABVs detected from the same bat species in Pennsylvania and New Jersey. The bat RABV (19-2898) detected from the migratory hoary bat belongs to the bats LC clade, clustering with the eleven viruses detected from the same species in Arizona, Washington, Idaho, and Tennessee. The approach used in this study generated novel data regarding genetic relationships of RABV variants, including their reservoirs, and their spatial origin and it would be useful as reference data for future investigations on RABV in North America. Continued surveillance and genome sequencing of bat RABV would be needed to monitor virus evolution and transmission, and to assess the emergence of genetic mutations that may be relevant for public health.
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spelling pubmed-87046782021-12-25 Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018–2019 Hyeon, Ji-Yeon Risatti, Guillermo R. Helal, Zeinab H. McGinnis, Holly Sims, Maureen Hunt, Amelia Chung, David H. Kim, Junwon Desiato, Julia Lee, Dong-Hun Viruses Communication We performed whole genome sequencing and genetic characterization of rabies viruses (RABV) detected in bats submitted to the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) during 2018–2019. Among 88 bats submitted to CVMDL, six brain samples (6.8%, 95% confidence interval: 1.6% to 12.1%) tested positive by direct fluorescent antibody test. RABVs were detected in big brown bats (Eptesicus fuscus, n = 4), a hoary bat (Lasiurus cinereus, n = 1), and an unidentified bat species (n = 1). Complete coding sequences of four out of six detected RABVs were obtained. In phylogenetic analysis, the RABVs (18-62, 18-4347, and 19-2274) from big brown bats belong to the bats EF-E1 clade, clustering with RABVs detected from the same bat species in Pennsylvania and New Jersey. The bat RABV (19-2898) detected from the migratory hoary bat belongs to the bats LC clade, clustering with the eleven viruses detected from the same species in Arizona, Washington, Idaho, and Tennessee. The approach used in this study generated novel data regarding genetic relationships of RABV variants, including their reservoirs, and their spatial origin and it would be useful as reference data for future investigations on RABV in North America. Continued surveillance and genome sequencing of bat RABV would be needed to monitor virus evolution and transmission, and to assess the emergence of genetic mutations that may be relevant for public health. MDPI 2021-12-13 /pmc/articles/PMC8704678/ /pubmed/34960769 http://dx.doi.org/10.3390/v13122500 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Hyeon, Ji-Yeon
Risatti, Guillermo R.
Helal, Zeinab H.
McGinnis, Holly
Sims, Maureen
Hunt, Amelia
Chung, David H.
Kim, Junwon
Desiato, Julia
Lee, Dong-Hun
Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018–2019
title Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018–2019
title_full Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018–2019
title_fullStr Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018–2019
title_full_unstemmed Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018–2019
title_short Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018–2019
title_sort whole genome sequencing and phylogenetic analysis of rabies viruses from bats in connecticut, usa, 2018–2019
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704678/
https://www.ncbi.nlm.nih.gov/pubmed/34960769
http://dx.doi.org/10.3390/v13122500
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