Cargando…

Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia

Research into ergot alkaloid production in major cereal cash crops is crucial for furthering our understanding of the potential toxicological impacts of Claviceps purpurea upon Canadian agriculture and to ensure consumer safety. An untargeted metabolomics approach profiling extracts of C. purpurea s...

Descripción completa

Detalles Bibliográficos
Autores principales: Hicks, Carmen, Witte, Thomas E., Sproule, Amanda, Lee, Tiah, Shoukouhi, Parivash, Popovic, Zlatko, Menzies, Jim G., Boddy, Christopher N., Liu, Miao, Overy, David P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704706/
https://www.ncbi.nlm.nih.gov/pubmed/34941699
http://dx.doi.org/10.3390/toxins13120861
_version_ 1784621771317575680
author Hicks, Carmen
Witte, Thomas E.
Sproule, Amanda
Lee, Tiah
Shoukouhi, Parivash
Popovic, Zlatko
Menzies, Jim G.
Boddy, Christopher N.
Liu, Miao
Overy, David P.
author_facet Hicks, Carmen
Witte, Thomas E.
Sproule, Amanda
Lee, Tiah
Shoukouhi, Parivash
Popovic, Zlatko
Menzies, Jim G.
Boddy, Christopher N.
Liu, Miao
Overy, David P.
author_sort Hicks, Carmen
collection PubMed
description Research into ergot alkaloid production in major cereal cash crops is crucial for furthering our understanding of the potential toxicological impacts of Claviceps purpurea upon Canadian agriculture and to ensure consumer safety. An untargeted metabolomics approach profiling extracts of C. purpurea sclerotia from four different grain crops separated the C. purpurea strains into two distinct metabolomic classes based on ergot alkaloid content. Variances in C. purpurea alkaloid profiles were correlated to genetic differences within the lpsA gene of the ergot alkaloid biosynthetic gene cluster from previously published genomes and from newly sequenced, long-read genome assemblies of Canadian strains. Based on gene cluster composition and unique polymorphisms, we hypothesize that the alkaloid content of C. purpurea sclerotia is currently undergoing adaptation. The patterns of lpsA gene diversity described in this small subset of Canadian strains provides a remarkable framework for understanding accelerated evolution of ergot alkaloid production in Claviceps purpurea.
format Online
Article
Text
id pubmed-8704706
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87047062021-12-25 Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia Hicks, Carmen Witte, Thomas E. Sproule, Amanda Lee, Tiah Shoukouhi, Parivash Popovic, Zlatko Menzies, Jim G. Boddy, Christopher N. Liu, Miao Overy, David P. Toxins (Basel) Article Research into ergot alkaloid production in major cereal cash crops is crucial for furthering our understanding of the potential toxicological impacts of Claviceps purpurea upon Canadian agriculture and to ensure consumer safety. An untargeted metabolomics approach profiling extracts of C. purpurea sclerotia from four different grain crops separated the C. purpurea strains into two distinct metabolomic classes based on ergot alkaloid content. Variances in C. purpurea alkaloid profiles were correlated to genetic differences within the lpsA gene of the ergot alkaloid biosynthetic gene cluster from previously published genomes and from newly sequenced, long-read genome assemblies of Canadian strains. Based on gene cluster composition and unique polymorphisms, we hypothesize that the alkaloid content of C. purpurea sclerotia is currently undergoing adaptation. The patterns of lpsA gene diversity described in this small subset of Canadian strains provides a remarkable framework for understanding accelerated evolution of ergot alkaloid production in Claviceps purpurea. MDPI 2021-12-02 /pmc/articles/PMC8704706/ /pubmed/34941699 http://dx.doi.org/10.3390/toxins13120861 Text en © 2021 by the authors and Her Majesty the Queen in Right of Canada as represented by the Minister of Agriculture and Agri-Food Canada; https://creativecommons.org/licenses/by/4.0/licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hicks, Carmen
Witte, Thomas E.
Sproule, Amanda
Lee, Tiah
Shoukouhi, Parivash
Popovic, Zlatko
Menzies, Jim G.
Boddy, Christopher N.
Liu, Miao
Overy, David P.
Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia
title Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia
title_full Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia
title_fullStr Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia
title_full_unstemmed Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia
title_short Evolution of the Ergot Alkaloid Biosynthetic Gene Cluster Results in Divergent Mycotoxin Profiles in Claviceps purpurea Sclerotia
title_sort evolution of the ergot alkaloid biosynthetic gene cluster results in divergent mycotoxin profiles in claviceps purpurea sclerotia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704706/
https://www.ncbi.nlm.nih.gov/pubmed/34941699
http://dx.doi.org/10.3390/toxins13120861
work_keys_str_mv AT hickscarmen evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT wittethomase evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT sprouleamanda evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT leetiah evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT shoukouhiparivash evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT popoviczlatko evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT menziesjimg evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT boddychristophern evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT liumiao evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia
AT overydavidp evolutionoftheergotalkaloidbiosyntheticgeneclusterresultsindivergentmycotoxinprofilesinclavicepspurpureasclerotia