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A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains

Combined potentiometric titration and isothermal titration calorimetry (ITC) methods were used to study the interactions of nickel(II) ions with the N-terminal fragments and histidine-rich fragments of Hpn-like protein from two Helicobacter pylori strains (11637 and 26695). The ITC measurements were...

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Autores principales: Witkowska, Danuta, Szebesczyk, Agnieszka, Wątły, Joanna, Braczkowski, Michał, Rowińska-Żyrek, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704837/
https://www.ncbi.nlm.nih.gov/pubmed/34948007
http://dx.doi.org/10.3390/ijms222413210
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author Witkowska, Danuta
Szebesczyk, Agnieszka
Wątły, Joanna
Braczkowski, Michał
Rowińska-Żyrek, Magdalena
author_facet Witkowska, Danuta
Szebesczyk, Agnieszka
Wątły, Joanna
Braczkowski, Michał
Rowińska-Żyrek, Magdalena
author_sort Witkowska, Danuta
collection PubMed
description Combined potentiometric titration and isothermal titration calorimetry (ITC) methods were used to study the interactions of nickel(II) ions with the N-terminal fragments and histidine-rich fragments of Hpn-like protein from two Helicobacter pylori strains (11637 and 26695). The ITC measurements were performed at various temperatures and buffers in order to extract proton-independent reaction enthalpies of nickel binding to each of the studied protein fragments. We bring up the problem of ITC results of nickel binding to the Hpn-like protein being not always compatible with those from potentiometry and MS regarding the stoichiometry and affinity. The roles of the ATCUN motif and multiple His and Gln residues in Ni(II) binding are discussed. The results provided the possibility to compare the Ni(II) binding properties between N-terminal and histidine-rich part of Hpn-like protein and between N-terminal parts of two Hpn-like strains, which differ mainly in the number of glutamine residues.
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spelling pubmed-87048372021-12-25 A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains Witkowska, Danuta Szebesczyk, Agnieszka Wątły, Joanna Braczkowski, Michał Rowińska-Żyrek, Magdalena Int J Mol Sci Article Combined potentiometric titration and isothermal titration calorimetry (ITC) methods were used to study the interactions of nickel(II) ions with the N-terminal fragments and histidine-rich fragments of Hpn-like protein from two Helicobacter pylori strains (11637 and 26695). The ITC measurements were performed at various temperatures and buffers in order to extract proton-independent reaction enthalpies of nickel binding to each of the studied protein fragments. We bring up the problem of ITC results of nickel binding to the Hpn-like protein being not always compatible with those from potentiometry and MS regarding the stoichiometry and affinity. The roles of the ATCUN motif and multiple His and Gln residues in Ni(II) binding are discussed. The results provided the possibility to compare the Ni(II) binding properties between N-terminal and histidine-rich part of Hpn-like protein and between N-terminal parts of two Hpn-like strains, which differ mainly in the number of glutamine residues. MDPI 2021-12-08 /pmc/articles/PMC8704837/ /pubmed/34948007 http://dx.doi.org/10.3390/ijms222413210 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Witkowska, Danuta
Szebesczyk, Agnieszka
Wątły, Joanna
Braczkowski, Michał
Rowińska-Żyrek, Magdalena
A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains
title A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains
title_full A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains
title_fullStr A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains
title_full_unstemmed A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains
title_short A Comparative Study on Nickel Binding to Hpn-like Polypeptides from Two Helicobacter pylori Strains
title_sort comparative study on nickel binding to hpn-like polypeptides from two helicobacter pylori strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704837/
https://www.ncbi.nlm.nih.gov/pubmed/34948007
http://dx.doi.org/10.3390/ijms222413210
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