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The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress
Oilseed rape (Brassica napus) is an economically important crop. In a temperate climate, powdery mildew Erysiphe crucifertaum can drastically reduce its yield. Nevertheless, cultivars resistant to this fungal disease have not yet been selected. Glutathione S-transferase GSTF11 is involved in glucosi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704923/ https://www.ncbi.nlm.nih.gov/pubmed/34961200 http://dx.doi.org/10.3390/plants10122729 |
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author | Mikhaylova, Elena Khusnutdinov, Emil Shein, Michael Yu Alekseev, Valentin Yu Nikonorov, Yuri Kuluev, Bulat |
author_facet | Mikhaylova, Elena Khusnutdinov, Emil Shein, Michael Yu Alekseev, Valentin Yu Nikonorov, Yuri Kuluev, Bulat |
author_sort | Mikhaylova, Elena |
collection | PubMed |
description | Oilseed rape (Brassica napus) is an economically important crop. In a temperate climate, powdery mildew Erysiphe crucifertaum can drastically reduce its yield. Nevertheless, cultivars resistant to this fungal disease have not yet been selected. Glutathione S-transferase GSTF11 is involved in glucosinolate (GSL) biosynthesis and response to stress, including fungal deceases. However, the impact of exogenous GSTF11 gene expression on resistance to powdery mildew has not yet been confirmed and requires further investigation. Transgenic B. napus was generated for this purpose. It demonstrated increased GST activity and a higher GSH:GSSG ratio under normal conditions. Powdery mildew Erysiphe crucifertaum caused 50% mortality in wild type (WT) plants. In most of transgenic plants, mycelium growth was inhibited. The infection contributed to higher GSTF11 expression and increased levels of glutathione (GSH) and oxidized glutathione (GSSG) in both transgenic and WT plants. In contrast, GSTF11 mRNA content, GST activity and GSSG level were lower only in WT plants. In transgenic plants, increased resistance to powdery mildew correlated with a lower GSH:GSSG ratio, indicating a higher content of neutralized toxic molecules. GSTF11 expression was also affected by cold stress, but not drought. At −1 °C, the expression level increased only in transgenic plants. Therefore, GSTF11 appears to be nonspecific and is able to protect plants under several types of stress. This gene could be used as a target in the production of stress tolerant cultivars. |
format | Online Article Text |
id | pubmed-8704923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87049232021-12-25 The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress Mikhaylova, Elena Khusnutdinov, Emil Shein, Michael Yu Alekseev, Valentin Yu Nikonorov, Yuri Kuluev, Bulat Plants (Basel) Article Oilseed rape (Brassica napus) is an economically important crop. In a temperate climate, powdery mildew Erysiphe crucifertaum can drastically reduce its yield. Nevertheless, cultivars resistant to this fungal disease have not yet been selected. Glutathione S-transferase GSTF11 is involved in glucosinolate (GSL) biosynthesis and response to stress, including fungal deceases. However, the impact of exogenous GSTF11 gene expression on resistance to powdery mildew has not yet been confirmed and requires further investigation. Transgenic B. napus was generated for this purpose. It demonstrated increased GST activity and a higher GSH:GSSG ratio under normal conditions. Powdery mildew Erysiphe crucifertaum caused 50% mortality in wild type (WT) plants. In most of transgenic plants, mycelium growth was inhibited. The infection contributed to higher GSTF11 expression and increased levels of glutathione (GSH) and oxidized glutathione (GSSG) in both transgenic and WT plants. In contrast, GSTF11 mRNA content, GST activity and GSSG level were lower only in WT plants. In transgenic plants, increased resistance to powdery mildew correlated with a lower GSH:GSSG ratio, indicating a higher content of neutralized toxic molecules. GSTF11 expression was also affected by cold stress, but not drought. At −1 °C, the expression level increased only in transgenic plants. Therefore, GSTF11 appears to be nonspecific and is able to protect plants under several types of stress. This gene could be used as a target in the production of stress tolerant cultivars. MDPI 2021-12-11 /pmc/articles/PMC8704923/ /pubmed/34961200 http://dx.doi.org/10.3390/plants10122729 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mikhaylova, Elena Khusnutdinov, Emil Shein, Michael Yu Alekseev, Valentin Yu Nikonorov, Yuri Kuluev, Bulat The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress |
title | The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress |
title_full | The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress |
title_fullStr | The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress |
title_full_unstemmed | The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress |
title_short | The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress |
title_sort | role of the gstf11 gene in resistance to powdery mildew infection and cold stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704923/ https://www.ncbi.nlm.nih.gov/pubmed/34961200 http://dx.doi.org/10.3390/plants10122729 |
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