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Antifungal Activity against Fusarium oxysporum of Botanical End-Products: An Integration of Chemical Composition and Antifungal Activity Datasets to Identify Antifungal Bioactives

Plants produce various compounds as defensive barriers to naturally control fungal diseases. Among them, vascular wilt caused by Fusarium oxysporum is one of the most destructive diseases in crops, causing relevant economic losses. The application of synthetic fungicides is the most used management...

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Autores principales: Cárdenas-Laverde, Diego, Barbosa-Cornelio, Ricardo, Coy-Barrera, Ericsson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705217/
https://www.ncbi.nlm.nih.gov/pubmed/34961034
http://dx.doi.org/10.3390/plants10122563
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author Cárdenas-Laverde, Diego
Barbosa-Cornelio, Ricardo
Coy-Barrera, Ericsson
author_facet Cárdenas-Laverde, Diego
Barbosa-Cornelio, Ricardo
Coy-Barrera, Ericsson
author_sort Cárdenas-Laverde, Diego
collection PubMed
description Plants produce various compounds as defensive barriers to naturally control fungal diseases. Among them, vascular wilt caused by Fusarium oxysporum is one of the most destructive diseases in crops, causing relevant economic losses. The application of synthetic fungicides is the most used management for this disease. However, this kind of method also involves adverse environmental impacts. Therefore, alternative methods are continuously being developed as a strategy to be involved in integrated pest management programs. Thus, as part of our research on antifungals of plant origin, a group of botanical extracts was assessed for the respective inhibitory effect on mycelium and conidia of F. oxysporum. Mycelial growth inhibition was measured in 12-well plates containing amended semi-solid medium, whereas conidial susceptibility was determined through microdilution. The identification of the bioactive compounds among test extracts was performed using an indirect approach, consisting of the integration of chemical composition and antifungal activity datasets through single-Y orthogonal partial least squares (OPLS) regression. Results showed that Piper aduncum extract was the most potent mycelial growth inhibitor whereas P. elongatum exhibited the best effect on conidia susceptibility. The active compounds identified through statistical integration and subsequent isolation were piperaduncin C, asebogenin and (−)-methyllinderatin. These findings indicated that the integrative, indirect approach is useful for the identification of bioactive metabolites from botanical extracts to be further used as biological protective agents against this phytopathogen.
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spelling pubmed-87052172021-12-25 Antifungal Activity against Fusarium oxysporum of Botanical End-Products: An Integration of Chemical Composition and Antifungal Activity Datasets to Identify Antifungal Bioactives Cárdenas-Laverde, Diego Barbosa-Cornelio, Ricardo Coy-Barrera, Ericsson Plants (Basel) Article Plants produce various compounds as defensive barriers to naturally control fungal diseases. Among them, vascular wilt caused by Fusarium oxysporum is one of the most destructive diseases in crops, causing relevant economic losses. The application of synthetic fungicides is the most used management for this disease. However, this kind of method also involves adverse environmental impacts. Therefore, alternative methods are continuously being developed as a strategy to be involved in integrated pest management programs. Thus, as part of our research on antifungals of plant origin, a group of botanical extracts was assessed for the respective inhibitory effect on mycelium and conidia of F. oxysporum. Mycelial growth inhibition was measured in 12-well plates containing amended semi-solid medium, whereas conidial susceptibility was determined through microdilution. The identification of the bioactive compounds among test extracts was performed using an indirect approach, consisting of the integration of chemical composition and antifungal activity datasets through single-Y orthogonal partial least squares (OPLS) regression. Results showed that Piper aduncum extract was the most potent mycelial growth inhibitor whereas P. elongatum exhibited the best effect on conidia susceptibility. The active compounds identified through statistical integration and subsequent isolation were piperaduncin C, asebogenin and (−)-methyllinderatin. These findings indicated that the integrative, indirect approach is useful for the identification of bioactive metabolites from botanical extracts to be further used as biological protective agents against this phytopathogen. MDPI 2021-11-24 /pmc/articles/PMC8705217/ /pubmed/34961034 http://dx.doi.org/10.3390/plants10122563 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cárdenas-Laverde, Diego
Barbosa-Cornelio, Ricardo
Coy-Barrera, Ericsson
Antifungal Activity against Fusarium oxysporum of Botanical End-Products: An Integration of Chemical Composition and Antifungal Activity Datasets to Identify Antifungal Bioactives
title Antifungal Activity against Fusarium oxysporum of Botanical End-Products: An Integration of Chemical Composition and Antifungal Activity Datasets to Identify Antifungal Bioactives
title_full Antifungal Activity against Fusarium oxysporum of Botanical End-Products: An Integration of Chemical Composition and Antifungal Activity Datasets to Identify Antifungal Bioactives
title_fullStr Antifungal Activity against Fusarium oxysporum of Botanical End-Products: An Integration of Chemical Composition and Antifungal Activity Datasets to Identify Antifungal Bioactives
title_full_unstemmed Antifungal Activity against Fusarium oxysporum of Botanical End-Products: An Integration of Chemical Composition and Antifungal Activity Datasets to Identify Antifungal Bioactives
title_short Antifungal Activity against Fusarium oxysporum of Botanical End-Products: An Integration of Chemical Composition and Antifungal Activity Datasets to Identify Antifungal Bioactives
title_sort antifungal activity against fusarium oxysporum of botanical end-products: an integration of chemical composition and antifungal activity datasets to identify antifungal bioactives
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705217/
https://www.ncbi.nlm.nih.gov/pubmed/34961034
http://dx.doi.org/10.3390/plants10122563
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