Cargando…

Antioxidant and Anti-Inflammatory Activity of Coffee Brew Evaluated after Simulated Gastrointestinal Digestion

Coffee contains human health-related molecules, namely polyphenols that possess a wide range of pharmacological functions, and their intake is associated with reduced colon cancer risk. This study aimed to assess the changes in the anti-inflammatory and antioxidant activity of coffee after simulated...

Descripción completa

Detalles Bibliográficos
Autores principales: Castaldo, Luigi, Toriello, Marianna, Sessa, Raffaele, Izzo, Luana, Lombardi, Sonia, Narváez, Alfonso, Ritieni, Alberto, Grosso, Michela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705407/
https://www.ncbi.nlm.nih.gov/pubmed/34959920
http://dx.doi.org/10.3390/nu13124368
_version_ 1784621938583273472
author Castaldo, Luigi
Toriello, Marianna
Sessa, Raffaele
Izzo, Luana
Lombardi, Sonia
Narváez, Alfonso
Ritieni, Alberto
Grosso, Michela
author_facet Castaldo, Luigi
Toriello, Marianna
Sessa, Raffaele
Izzo, Luana
Lombardi, Sonia
Narváez, Alfonso
Ritieni, Alberto
Grosso, Michela
author_sort Castaldo, Luigi
collection PubMed
description Coffee contains human health-related molecules, namely polyphenols that possess a wide range of pharmacological functions, and their intake is associated with reduced colon cancer risk. This study aimed to assess the changes in the anti-inflammatory and antioxidant activity of coffee after simulated gastrointestinal digestion. The evaluation of intracellular reactive oxygen species (ROS) levels in the HT-29 human colon cancer cell line and three in vitro spectrophotometric assays were performed to determine the antioxidant activity of the samples. Characterization of coffee composition was also assessed through a Q-Orbitrap high-resolution mass spectrometry analysis. The results highlighted that the levels of polyphenols in the digested coffee brews were higher than those of the non-digested ones. All assayed samples decreased the levels of intracellular ROS when compared to untreated cells, while digested coffee samples exhibited higher antioxidant capacity and total phenolic content than not-digested coffee samples. Digested coffee samples showed a higher reduction in interleukin-6 levels than the not-digested samples in lipopolysaccharide-stimulated HT-29 cells treated for 48 h and fewer cytotoxic effects in the MTT assay. Overall, our findings suggest that coffee may exert antioxidant and anti-inflammatory properties, and the digestion process may be able to release compounds with higher bioactivity.
format Online
Article
Text
id pubmed-8705407
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87054072021-12-25 Antioxidant and Anti-Inflammatory Activity of Coffee Brew Evaluated after Simulated Gastrointestinal Digestion Castaldo, Luigi Toriello, Marianna Sessa, Raffaele Izzo, Luana Lombardi, Sonia Narváez, Alfonso Ritieni, Alberto Grosso, Michela Nutrients Article Coffee contains human health-related molecules, namely polyphenols that possess a wide range of pharmacological functions, and their intake is associated with reduced colon cancer risk. This study aimed to assess the changes in the anti-inflammatory and antioxidant activity of coffee after simulated gastrointestinal digestion. The evaluation of intracellular reactive oxygen species (ROS) levels in the HT-29 human colon cancer cell line and three in vitro spectrophotometric assays were performed to determine the antioxidant activity of the samples. Characterization of coffee composition was also assessed through a Q-Orbitrap high-resolution mass spectrometry analysis. The results highlighted that the levels of polyphenols in the digested coffee brews were higher than those of the non-digested ones. All assayed samples decreased the levels of intracellular ROS when compared to untreated cells, while digested coffee samples exhibited higher antioxidant capacity and total phenolic content than not-digested coffee samples. Digested coffee samples showed a higher reduction in interleukin-6 levels than the not-digested samples in lipopolysaccharide-stimulated HT-29 cells treated for 48 h and fewer cytotoxic effects in the MTT assay. Overall, our findings suggest that coffee may exert antioxidant and anti-inflammatory properties, and the digestion process may be able to release compounds with higher bioactivity. MDPI 2021-12-05 /pmc/articles/PMC8705407/ /pubmed/34959920 http://dx.doi.org/10.3390/nu13124368 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Castaldo, Luigi
Toriello, Marianna
Sessa, Raffaele
Izzo, Luana
Lombardi, Sonia
Narváez, Alfonso
Ritieni, Alberto
Grosso, Michela
Antioxidant and Anti-Inflammatory Activity of Coffee Brew Evaluated after Simulated Gastrointestinal Digestion
title Antioxidant and Anti-Inflammatory Activity of Coffee Brew Evaluated after Simulated Gastrointestinal Digestion
title_full Antioxidant and Anti-Inflammatory Activity of Coffee Brew Evaluated after Simulated Gastrointestinal Digestion
title_fullStr Antioxidant and Anti-Inflammatory Activity of Coffee Brew Evaluated after Simulated Gastrointestinal Digestion
title_full_unstemmed Antioxidant and Anti-Inflammatory Activity of Coffee Brew Evaluated after Simulated Gastrointestinal Digestion
title_short Antioxidant and Anti-Inflammatory Activity of Coffee Brew Evaluated after Simulated Gastrointestinal Digestion
title_sort antioxidant and anti-inflammatory activity of coffee brew evaluated after simulated gastrointestinal digestion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705407/
https://www.ncbi.nlm.nih.gov/pubmed/34959920
http://dx.doi.org/10.3390/nu13124368
work_keys_str_mv AT castaldoluigi antioxidantandantiinflammatoryactivityofcoffeebrewevaluatedaftersimulatedgastrointestinaldigestion
AT toriellomarianna antioxidantandantiinflammatoryactivityofcoffeebrewevaluatedaftersimulatedgastrointestinaldigestion
AT sessaraffaele antioxidantandantiinflammatoryactivityofcoffeebrewevaluatedaftersimulatedgastrointestinaldigestion
AT izzoluana antioxidantandantiinflammatoryactivityofcoffeebrewevaluatedaftersimulatedgastrointestinaldigestion
AT lombardisonia antioxidantandantiinflammatoryactivityofcoffeebrewevaluatedaftersimulatedgastrointestinaldigestion
AT narvaezalfonso antioxidantandantiinflammatoryactivityofcoffeebrewevaluatedaftersimulatedgastrointestinaldigestion
AT ritienialberto antioxidantandantiinflammatoryactivityofcoffeebrewevaluatedaftersimulatedgastrointestinaldigestion
AT grossomichela antioxidantandantiinflammatoryactivityofcoffeebrewevaluatedaftersimulatedgastrointestinaldigestion