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Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan

Toxic crabs of the family Xanthidae contain saxitoxins (STXs) and/or tetrodotoxin (TTX), but the toxin ratio differs depending on their habitat. In the present study, to clarify within reef variations in the toxin profile of xanthid crabs, we collected specimens of the toxic xanthid crab Zosimus aen...

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Autores principales: Zhang, Yuchengmin, Tsutsui, Hideto, Yamawaki, Nobuhiro, Morii, Yasuhiro, Nishihara, Gregory N., Itoi, Shiro, Arakawa, Osamu, Takatani, Tomohiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705613/
https://www.ncbi.nlm.nih.gov/pubmed/34940669
http://dx.doi.org/10.3390/md19120670
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author Zhang, Yuchengmin
Tsutsui, Hideto
Yamawaki, Nobuhiro
Morii, Yasuhiro
Nishihara, Gregory N.
Itoi, Shiro
Arakawa, Osamu
Takatani, Tomohiro
author_facet Zhang, Yuchengmin
Tsutsui, Hideto
Yamawaki, Nobuhiro
Morii, Yasuhiro
Nishihara, Gregory N.
Itoi, Shiro
Arakawa, Osamu
Takatani, Tomohiro
author_sort Zhang, Yuchengmin
collection PubMed
description Toxic crabs of the family Xanthidae contain saxitoxins (STXs) and/or tetrodotoxin (TTX), but the toxin ratio differs depending on their habitat. In the present study, to clarify within reef variations in the toxin profile of xanthid crabs, we collected specimens of the toxic xanthid crab Zosimus aeneus and their sampling location within a single reef (Yoshihara reef) on Ishigaki Island, Okinawa Prefecture, Japan, in 2018 and 2019. The STXs/TTX content within the appendages and viscera or stomach contents of each specimen was determined by instrumental analyses. Our findings revealed the existence of three zones in Yoshihara reef; one in which many individuals accumulate extremely high concentrations of STXs (northwestern part of the reef; NW zone), another in which individuals generally have small amounts of TTX but little STXs (central part of the reef; CTR zone), and a third in which individuals generally exhibit intermediate characteristics (southeastern part of the reef; SE zone). Furthermore, light microscopic observations of the stomach contents of crab specimens collected from the NW and CTR zones revealed that ascidian spicules of the genus Lissoclinum were dominant in the NW zone, whereas those of the genus Trididemnum were dominant in the CTR zone. Although the toxicity of these ascidians is unknown, Lissoclinum ascidians are considered good candidate source organisms of STXs harbored by toxic xanthid crabs.
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spelling pubmed-87056132021-12-25 Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan Zhang, Yuchengmin Tsutsui, Hideto Yamawaki, Nobuhiro Morii, Yasuhiro Nishihara, Gregory N. Itoi, Shiro Arakawa, Osamu Takatani, Tomohiro Mar Drugs Article Toxic crabs of the family Xanthidae contain saxitoxins (STXs) and/or tetrodotoxin (TTX), but the toxin ratio differs depending on their habitat. In the present study, to clarify within reef variations in the toxin profile of xanthid crabs, we collected specimens of the toxic xanthid crab Zosimus aeneus and their sampling location within a single reef (Yoshihara reef) on Ishigaki Island, Okinawa Prefecture, Japan, in 2018 and 2019. The STXs/TTX content within the appendages and viscera or stomach contents of each specimen was determined by instrumental analyses. Our findings revealed the existence of three zones in Yoshihara reef; one in which many individuals accumulate extremely high concentrations of STXs (northwestern part of the reef; NW zone), another in which individuals generally have small amounts of TTX but little STXs (central part of the reef; CTR zone), and a third in which individuals generally exhibit intermediate characteristics (southeastern part of the reef; SE zone). Furthermore, light microscopic observations of the stomach contents of crab specimens collected from the NW and CTR zones revealed that ascidian spicules of the genus Lissoclinum were dominant in the NW zone, whereas those of the genus Trididemnum were dominant in the CTR zone. Although the toxicity of these ascidians is unknown, Lissoclinum ascidians are considered good candidate source organisms of STXs harbored by toxic xanthid crabs. MDPI 2021-11-26 /pmc/articles/PMC8705613/ /pubmed/34940669 http://dx.doi.org/10.3390/md19120670 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yuchengmin
Tsutsui, Hideto
Yamawaki, Nobuhiro
Morii, Yasuhiro
Nishihara, Gregory N.
Itoi, Shiro
Arakawa, Osamu
Takatani, Tomohiro
Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan
title Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan
title_full Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan
title_fullStr Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan
title_full_unstemmed Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan
title_short Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan
title_sort geographic variations in the toxin profile of the xanthid crab zosimus aeneus in a single reef on ishigaki island, okinawa, japan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705613/
https://www.ncbi.nlm.nih.gov/pubmed/34940669
http://dx.doi.org/10.3390/md19120670
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