Cargando…

NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn)

Starch is an important component in lotus. ABA is an important plant hormone, which plays a very crucial role in regulating plant starch synthesis. Using ‘MRH’ as experimental materials, the leaves were sprayed with exogenous ABA before the rhizome expansion. The results showed that stomatal conduct...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Peng, Liu, Ailian, Zhang, Yongyan, Feng, Kai, Zhao, Shuping, Li, Liangjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705639/
https://www.ncbi.nlm.nih.gov/pubmed/34948298
http://dx.doi.org/10.3390/ijms222413506
_version_ 1784621996383928320
author Wu, Peng
Liu, Ailian
Zhang, Yongyan
Feng, Kai
Zhao, Shuping
Li, Liangjun
author_facet Wu, Peng
Liu, Ailian
Zhang, Yongyan
Feng, Kai
Zhao, Shuping
Li, Liangjun
author_sort Wu, Peng
collection PubMed
description Starch is an important component in lotus. ABA is an important plant hormone, which plays a very crucial role in regulating plant starch synthesis. Using ‘MRH’ as experimental materials, the leaves were sprayed with exogenous ABA before the rhizome expansion. The results showed that stomatal conductance and transpiration rate decreased while net photosynthetic rate increased. The total starch content of the underground rhizome of lotus increased significantly. Meanwhile, qPCR results showed that the relative expression levels of NnSS1, NnSBE1 and NnABI4 were all upregulated after ABA treatment. Then, yeast one-hybrid and dual luciferase assay suggested that NnABI4 protein can promote the expression of NnSS1 by directly binding to its promoter. In addition, subcellular localization results showed that NnABI4 encodes a nuclear protein, and NnSS1 protein was located in the chloroplast. Finally, these results indicate that ABA induced the upregulated expression of NnABI4, and NnABI4 promoted the expression of NnSS1 and thus enhanced starch accumulation in lotus rhizomes. This will provide a theoretical basis for studying the molecular mechanism of ABA regulating starch synthesis in plant.
format Online
Article
Text
id pubmed-8705639
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87056392021-12-25 NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn) Wu, Peng Liu, Ailian Zhang, Yongyan Feng, Kai Zhao, Shuping Li, Liangjun Int J Mol Sci Article Starch is an important component in lotus. ABA is an important plant hormone, which plays a very crucial role in regulating plant starch synthesis. Using ‘MRH’ as experimental materials, the leaves were sprayed with exogenous ABA before the rhizome expansion. The results showed that stomatal conductance and transpiration rate decreased while net photosynthetic rate increased. The total starch content of the underground rhizome of lotus increased significantly. Meanwhile, qPCR results showed that the relative expression levels of NnSS1, NnSBE1 and NnABI4 were all upregulated after ABA treatment. Then, yeast one-hybrid and dual luciferase assay suggested that NnABI4 protein can promote the expression of NnSS1 by directly binding to its promoter. In addition, subcellular localization results showed that NnABI4 encodes a nuclear protein, and NnSS1 protein was located in the chloroplast. Finally, these results indicate that ABA induced the upregulated expression of NnABI4, and NnABI4 promoted the expression of NnSS1 and thus enhanced starch accumulation in lotus rhizomes. This will provide a theoretical basis for studying the molecular mechanism of ABA regulating starch synthesis in plant. MDPI 2021-12-16 /pmc/articles/PMC8705639/ /pubmed/34948298 http://dx.doi.org/10.3390/ijms222413506 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Peng
Liu, Ailian
Zhang, Yongyan
Feng, Kai
Zhao, Shuping
Li, Liangjun
NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn)
title NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn)
title_full NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn)
title_fullStr NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn)
title_full_unstemmed NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn)
title_short NnABI4-Mediated ABA Regulation of Starch Biosynthesis in Lotus (Nelumbo nucifera Gaertn)
title_sort nnabi4-mediated aba regulation of starch biosynthesis in lotus (nelumbo nucifera gaertn)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705639/
https://www.ncbi.nlm.nih.gov/pubmed/34948298
http://dx.doi.org/10.3390/ijms222413506
work_keys_str_mv AT wupeng nnabi4mediatedabaregulationofstarchbiosynthesisinlotusnelumbonuciferagaertn
AT liuailian nnabi4mediatedabaregulationofstarchbiosynthesisinlotusnelumbonuciferagaertn
AT zhangyongyan nnabi4mediatedabaregulationofstarchbiosynthesisinlotusnelumbonuciferagaertn
AT fengkai nnabi4mediatedabaregulationofstarchbiosynthesisinlotusnelumbonuciferagaertn
AT zhaoshuping nnabi4mediatedabaregulationofstarchbiosynthesisinlotusnelumbonuciferagaertn
AT liliangjun nnabi4mediatedabaregulationofstarchbiosynthesisinlotusnelumbonuciferagaertn