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Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices

We present a novel methodology based on ion conductance to evaluate the perfusability of vascular vessels in microfluidic devices without microscopic imaging. The devices consisted of five channels, with the center channel filled with fibrin/collagen gel containing human umbilical vein endothelial c...

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Detalles Bibliográficos
Autores principales: Akasaka, Rise, Ozawa, Masashi, Nashimoto, Yuji, Ino, Kosuke, Shiku, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705798/
https://www.ncbi.nlm.nih.gov/pubmed/34945341
http://dx.doi.org/10.3390/mi12121491
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author Akasaka, Rise
Ozawa, Masashi
Nashimoto, Yuji
Ino, Kosuke
Shiku, Hitoshi
author_facet Akasaka, Rise
Ozawa, Masashi
Nashimoto, Yuji
Ino, Kosuke
Shiku, Hitoshi
author_sort Akasaka, Rise
collection PubMed
description We present a novel methodology based on ion conductance to evaluate the perfusability of vascular vessels in microfluidic devices without microscopic imaging. The devices consisted of five channels, with the center channel filled with fibrin/collagen gel containing human umbilical vein endothelial cells (HUVECs). Fibroblasts were cultured in the other channels to improve the vascular network formation. To form vessel structures bridging the center channel, HUVEC monolayers were prepared on both side walls of the gel. During the culture, the HUVECs migrated from the monolayer and connected to the HUVECs in the gel, and vascular vessels formed, resulting in successful perfusion between the channels after culturing for 3–5 d. To evaluate perfusion without microscopic imaging, Ag/AgCl wires were inserted into the channels, and ion currents were obtained to measure the ion conductance between the channels separated by the HUVEC monolayers. As the HUVEC monolayers blocked the ion current flow, the ion currents were low before vessel formation. In contrast, ion currents increased after vessel formation because of creation of ion current paths. Thus, the observed ion currents were correlated with the perfusability of the vessels, indicating that they can be used as indicators of perfusion during vessel formation in microfluidic devices. The developed methodology will be used for drug screening using organs-on-a-chip containing vascular vessels.
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spelling pubmed-87057982021-12-25 Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices Akasaka, Rise Ozawa, Masashi Nashimoto, Yuji Ino, Kosuke Shiku, Hitoshi Micromachines (Basel) Article We present a novel methodology based on ion conductance to evaluate the perfusability of vascular vessels in microfluidic devices without microscopic imaging. The devices consisted of five channels, with the center channel filled with fibrin/collagen gel containing human umbilical vein endothelial cells (HUVECs). Fibroblasts were cultured in the other channels to improve the vascular network formation. To form vessel structures bridging the center channel, HUVEC monolayers were prepared on both side walls of the gel. During the culture, the HUVECs migrated from the monolayer and connected to the HUVECs in the gel, and vascular vessels formed, resulting in successful perfusion between the channels after culturing for 3–5 d. To evaluate perfusion without microscopic imaging, Ag/AgCl wires were inserted into the channels, and ion currents were obtained to measure the ion conductance between the channels separated by the HUVEC monolayers. As the HUVEC monolayers blocked the ion current flow, the ion currents were low before vessel formation. In contrast, ion currents increased after vessel formation because of creation of ion current paths. Thus, the observed ion currents were correlated with the perfusability of the vessels, indicating that they can be used as indicators of perfusion during vessel formation in microfluidic devices. The developed methodology will be used for drug screening using organs-on-a-chip containing vascular vessels. MDPI 2021-11-30 /pmc/articles/PMC8705798/ /pubmed/34945341 http://dx.doi.org/10.3390/mi12121491 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akasaka, Rise
Ozawa, Masashi
Nashimoto, Yuji
Ino, Kosuke
Shiku, Hitoshi
Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
title Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
title_full Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
title_fullStr Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
title_full_unstemmed Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
title_short Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices
title_sort ion conductance-based perfusability assay of vascular vessel models in microfluidic devices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705798/
https://www.ncbi.nlm.nih.gov/pubmed/34945341
http://dx.doi.org/10.3390/mi12121491
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