Cargando…
Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis
In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglycer...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705906/ https://www.ncbi.nlm.nih.gov/pubmed/34959999 http://dx.doi.org/10.3390/nu13124448 |
_version_ | 1784622062231355392 |
---|---|
author | Park, Woo Yong Song, Gahee Noh, Joon Hak Kim, Taegon Kim, Jae Jin Hong, Seokbeom Park, Jinbong Um, Jae-Young |
author_facet | Park, Woo Yong Song, Gahee Noh, Joon Hak Kim, Taegon Kim, Jae Jin Hong, Seokbeom Park, Jinbong Um, Jae-Young |
author_sort | Park, Woo Yong |
collection | PubMed |
description | In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis. |
format | Online Article Text |
id | pubmed-8705906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87059062021-12-25 Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis Park, Woo Yong Song, Gahee Noh, Joon Hak Kim, Taegon Kim, Jae Jin Hong, Seokbeom Park, Jinbong Um, Jae-Young Nutrients Article In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis. MDPI 2021-12-13 /pmc/articles/PMC8705906/ /pubmed/34959999 http://dx.doi.org/10.3390/nu13124448 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Woo Yong Song, Gahee Noh, Joon Hak Kim, Taegon Kim, Jae Jin Hong, Seokbeom Park, Jinbong Um, Jae-Young Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
title | Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
title_full | Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
title_fullStr | Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
title_full_unstemmed | Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
title_short | Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis |
title_sort | raphani semen (raphanus sativus l.) ameliorates alcoholic fatty liver disease by regulating de novo lipogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705906/ https://www.ncbi.nlm.nih.gov/pubmed/34959999 http://dx.doi.org/10.3390/nu13124448 |
work_keys_str_mv | AT parkwooyong raphanisemenraphanussativuslamelioratesalcoholicfattyliverdiseasebyregulatingdenovolipogenesis AT songgahee raphanisemenraphanussativuslamelioratesalcoholicfattyliverdiseasebyregulatingdenovolipogenesis AT nohjoonhak raphanisemenraphanussativuslamelioratesalcoholicfattyliverdiseasebyregulatingdenovolipogenesis AT kimtaegon raphanisemenraphanussativuslamelioratesalcoholicfattyliverdiseasebyregulatingdenovolipogenesis AT kimjaejin raphanisemenraphanussativuslamelioratesalcoholicfattyliverdiseasebyregulatingdenovolipogenesis AT hongseokbeom raphanisemenraphanussativuslamelioratesalcoholicfattyliverdiseasebyregulatingdenovolipogenesis AT parkjinbong raphanisemenraphanussativuslamelioratesalcoholicfattyliverdiseasebyregulatingdenovolipogenesis AT umjaeyoung raphanisemenraphanussativuslamelioratesalcoholicfattyliverdiseasebyregulatingdenovolipogenesis |