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Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis

In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglycer...

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Autores principales: Park, Woo Yong, Song, Gahee, Noh, Joon Hak, Kim, Taegon, Kim, Jae Jin, Hong, Seokbeom, Park, Jinbong, Um, Jae-Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705906/
https://www.ncbi.nlm.nih.gov/pubmed/34959999
http://dx.doi.org/10.3390/nu13124448
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author Park, Woo Yong
Song, Gahee
Noh, Joon Hak
Kim, Taegon
Kim, Jae Jin
Hong, Seokbeom
Park, Jinbong
Um, Jae-Young
author_facet Park, Woo Yong
Song, Gahee
Noh, Joon Hak
Kim, Taegon
Kim, Jae Jin
Hong, Seokbeom
Park, Jinbong
Um, Jae-Young
author_sort Park, Woo Yong
collection PubMed
description In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis.
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spelling pubmed-87059062021-12-25 Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis Park, Woo Yong Song, Gahee Noh, Joon Hak Kim, Taegon Kim, Jae Jin Hong, Seokbeom Park, Jinbong Um, Jae-Young Nutrients Article In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis. MDPI 2021-12-13 /pmc/articles/PMC8705906/ /pubmed/34959999 http://dx.doi.org/10.3390/nu13124448 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Woo Yong
Song, Gahee
Noh, Joon Hak
Kim, Taegon
Kim, Jae Jin
Hong, Seokbeom
Park, Jinbong
Um, Jae-Young
Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis
title Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis
title_full Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis
title_fullStr Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis
title_full_unstemmed Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis
title_short Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis
title_sort raphani semen (raphanus sativus l.) ameliorates alcoholic fatty liver disease by regulating de novo lipogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705906/
https://www.ncbi.nlm.nih.gov/pubmed/34959999
http://dx.doi.org/10.3390/nu13124448
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