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Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer

The transmission of chronic wasting disease (CWD) has largely been attributed to contact with infectious prions shed in excretions (saliva, urine, feces, blood) by direct animal-to-animal exposure or indirect contact with the environment. Less-well studied has been the role that mother-to-offspring...

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Autores principales: Nalls, Amy V., McNulty, Erin E., Mayfield, Amber, Crum, James M., Keel, Michael K., Hoover, Edward A., Ruder, Mark G., Mathiason, Candace K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705995/
https://www.ncbi.nlm.nih.gov/pubmed/34960698
http://dx.doi.org/10.3390/v13122430
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author Nalls, Amy V.
McNulty, Erin E.
Mayfield, Amber
Crum, James M.
Keel, Michael K.
Hoover, Edward A.
Ruder, Mark G.
Mathiason, Candace K.
author_facet Nalls, Amy V.
McNulty, Erin E.
Mayfield, Amber
Crum, James M.
Keel, Michael K.
Hoover, Edward A.
Ruder, Mark G.
Mathiason, Candace K.
author_sort Nalls, Amy V.
collection PubMed
description The transmission of chronic wasting disease (CWD) has largely been attributed to contact with infectious prions shed in excretions (saliva, urine, feces, blood) by direct animal-to-animal exposure or indirect contact with the environment. Less-well studied has been the role that mother-to-offspring transmission may play in the facile transmission of CWD, and whether mother-to-offspring transmission before birth may contribute to the extensive spread of CWD. We thereby focused on a population of free-ranging white-tailed deer from West Virginia, USA, in which CWD has been detected. Fetal tissues, ranging from 113 to 158 days of gestation, were harvested from the uteri of CWD+ dams in the asymptomatic phase of infection. Using serial protein misfolding amplification (sPMCA), we detected evidence of prion seeds in 7 of 14 fetuses (50%) from 7 of 9 pregnancies (78%), with the earliest detection at 113 gestational days. This is the first report of CWD detection in free ranging white-tailed deer fetal tissues. Further investigation within cervid populations across North America will help define the role and impact of mother-to-offspring vertical transmission of CWD.
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spelling pubmed-87059952021-12-25 Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer Nalls, Amy V. McNulty, Erin E. Mayfield, Amber Crum, James M. Keel, Michael K. Hoover, Edward A. Ruder, Mark G. Mathiason, Candace K. Viruses Article The transmission of chronic wasting disease (CWD) has largely been attributed to contact with infectious prions shed in excretions (saliva, urine, feces, blood) by direct animal-to-animal exposure or indirect contact with the environment. Less-well studied has been the role that mother-to-offspring transmission may play in the facile transmission of CWD, and whether mother-to-offspring transmission before birth may contribute to the extensive spread of CWD. We thereby focused on a population of free-ranging white-tailed deer from West Virginia, USA, in which CWD has been detected. Fetal tissues, ranging from 113 to 158 days of gestation, were harvested from the uteri of CWD+ dams in the asymptomatic phase of infection. Using serial protein misfolding amplification (sPMCA), we detected evidence of prion seeds in 7 of 14 fetuses (50%) from 7 of 9 pregnancies (78%), with the earliest detection at 113 gestational days. This is the first report of CWD detection in free ranging white-tailed deer fetal tissues. Further investigation within cervid populations across North America will help define the role and impact of mother-to-offspring vertical transmission of CWD. MDPI 2021-12-03 /pmc/articles/PMC8705995/ /pubmed/34960698 http://dx.doi.org/10.3390/v13122430 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nalls, Amy V.
McNulty, Erin E.
Mayfield, Amber
Crum, James M.
Keel, Michael K.
Hoover, Edward A.
Ruder, Mark G.
Mathiason, Candace K.
Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer
title Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer
title_full Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer
title_fullStr Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer
title_full_unstemmed Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer
title_short Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer
title_sort detection of chronic wasting disease prions in fetal tissues of free-ranging white-tailed deer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705995/
https://www.ncbi.nlm.nih.gov/pubmed/34960698
http://dx.doi.org/10.3390/v13122430
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