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Multiple Ion Scaffold-Based Delivery Platform for Potential Application in Early Stages of Bone Regeneration

Bone has the intrinsic capacity to regenerate itself, as long as the damage is small, through the sequential stimulation of specific phases, such as angiogenesis followed by osteogenesis. However, when the damage is extensive it is unable to regenerate and bone tissue engineering is used as an alter...

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Autores principales: Bosch-Rué, Èlia, Díez-Tercero, Leire, Rodriguez-Gonzalez, Raquel, Pérez, Román A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706177/
https://www.ncbi.nlm.nih.gov/pubmed/34947272
http://dx.doi.org/10.3390/ma14247676
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author Bosch-Rué, Èlia
Díez-Tercero, Leire
Rodriguez-Gonzalez, Raquel
Pérez, Román A.
author_facet Bosch-Rué, Èlia
Díez-Tercero, Leire
Rodriguez-Gonzalez, Raquel
Pérez, Román A.
author_sort Bosch-Rué, Èlia
collection PubMed
description Bone has the intrinsic capacity to regenerate itself, as long as the damage is small, through the sequential stimulation of specific phases, such as angiogenesis followed by osteogenesis. However, when the damage is extensive it is unable to regenerate and bone tissue engineering is used as an alternative. In this study, we developed a platform to allow the triple ion delivery with sequential delivery capacity to potentially stimulate antibacterial, angiogenic and osteogenic processes. The scaffold-based platform consisted of alginate/hydroxyapatite (HA) microparticles embedded in alginate fibers. Firstly, microparticles were developed using different ratios of alginate:HA using the spraying method, resulting in a high reproducibility of the technique. Microparticle size between 100–300 µm and ratio 1:40 resulted in a more spherical morphology and were selected for their incorporation into alginate fiber. Different amounts of copper and cobalt were added with the microparticles and alginate fiber, respectively, were used as model ions which could eventually modulate and mimic antimicrobial and angiogenic processes. Moreover, calcium ion was also incorporated in both, in order to provide the system with potential osteogenic properties together with HA. The multiple delivery of copper, cobalt and calcium released were in the therapeutic range as measured by induced coupled plasma (ICP), providing a promising delivery strategy for tissue engineering.
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spelling pubmed-87061772021-12-25 Multiple Ion Scaffold-Based Delivery Platform for Potential Application in Early Stages of Bone Regeneration Bosch-Rué, Èlia Díez-Tercero, Leire Rodriguez-Gonzalez, Raquel Pérez, Román A. Materials (Basel) Article Bone has the intrinsic capacity to regenerate itself, as long as the damage is small, through the sequential stimulation of specific phases, such as angiogenesis followed by osteogenesis. However, when the damage is extensive it is unable to regenerate and bone tissue engineering is used as an alternative. In this study, we developed a platform to allow the triple ion delivery with sequential delivery capacity to potentially stimulate antibacterial, angiogenic and osteogenic processes. The scaffold-based platform consisted of alginate/hydroxyapatite (HA) microparticles embedded in alginate fibers. Firstly, microparticles were developed using different ratios of alginate:HA using the spraying method, resulting in a high reproducibility of the technique. Microparticle size between 100–300 µm and ratio 1:40 resulted in a more spherical morphology and were selected for their incorporation into alginate fiber. Different amounts of copper and cobalt were added with the microparticles and alginate fiber, respectively, were used as model ions which could eventually modulate and mimic antimicrobial and angiogenic processes. Moreover, calcium ion was also incorporated in both, in order to provide the system with potential osteogenic properties together with HA. The multiple delivery of copper, cobalt and calcium released were in the therapeutic range as measured by induced coupled plasma (ICP), providing a promising delivery strategy for tissue engineering. MDPI 2021-12-13 /pmc/articles/PMC8706177/ /pubmed/34947272 http://dx.doi.org/10.3390/ma14247676 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bosch-Rué, Èlia
Díez-Tercero, Leire
Rodriguez-Gonzalez, Raquel
Pérez, Román A.
Multiple Ion Scaffold-Based Delivery Platform for Potential Application in Early Stages of Bone Regeneration
title Multiple Ion Scaffold-Based Delivery Platform for Potential Application in Early Stages of Bone Regeneration
title_full Multiple Ion Scaffold-Based Delivery Platform for Potential Application in Early Stages of Bone Regeneration
title_fullStr Multiple Ion Scaffold-Based Delivery Platform for Potential Application in Early Stages of Bone Regeneration
title_full_unstemmed Multiple Ion Scaffold-Based Delivery Platform for Potential Application in Early Stages of Bone Regeneration
title_short Multiple Ion Scaffold-Based Delivery Platform for Potential Application in Early Stages of Bone Regeneration
title_sort multiple ion scaffold-based delivery platform for potential application in early stages of bone regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706177/
https://www.ncbi.nlm.nih.gov/pubmed/34947272
http://dx.doi.org/10.3390/ma14247676
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