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Xerotolerance: A New Property in Exiguobacterium Genus
The highly xerotolerant bacterium classified as Exiguobacterium sp. Helios isolated from a solar panel in Spain showed a close relationship to Exiguobacterium sibiricum 255-15 isolated from Siberian permafrost. Xerotolerance has not been previously described as a characteristic of the extremely dive...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706201/ https://www.ncbi.nlm.nih.gov/pubmed/34946057 http://dx.doi.org/10.3390/microorganisms9122455 |
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author | López, María Castillo Galán, Beatriz Carmona, Manuel Navarro Llorens, Juana María Peretó, Juli Porcar, Manuel Getino, Luis Olivera, Elías R. Luengo, José M. Castro, Laura García, José Luís |
author_facet | López, María Castillo Galán, Beatriz Carmona, Manuel Navarro Llorens, Juana María Peretó, Juli Porcar, Manuel Getino, Luis Olivera, Elías R. Luengo, José M. Castro, Laura García, José Luís |
author_sort | López, María Castillo |
collection | PubMed |
description | The highly xerotolerant bacterium classified as Exiguobacterium sp. Helios isolated from a solar panel in Spain showed a close relationship to Exiguobacterium sibiricum 255-15 isolated from Siberian permafrost. Xerotolerance has not been previously described as a characteristic of the extremely diverse Exiguobacterium genus, but both strains Helios and 255-15 showed higher xerotolerance than that described in the reference xerotolerant model strain Deinococcus radiodurans. Significant changes observed in the cell morphology after their desiccation suggests that the structure of cellular surface plays an important role in xerotolerance. Apart from its remarkable resistance to desiccation, Exiguobacterium sp. Helios strain shows several polyextremophilic characteristics that make it a promising chassis for biotechnological applications. Exiguobacterium sp. Helios cells produce nanoparticles of selenium in the presence of selenite linked to its resistance mechanism. Using the Lactobacillus plasmid pRCR12 that harbors a cherry marker, we have developed a transformation protocol for Exiguobacterium sp. Helios strain, being the first time that a bacterium of Exiguobacterium genus has been genetically modified. The comparison of Exiguobacterium sp. Helios and E. sibiricum 255-15 genomes revealed several interesting similarities and differences. Both strains contain a complete set of competence-related DNA transformation genes, suggesting that they might have natural competence, and an incomplete set of genes involved in sporulation; moreover, these strains not produce spores, suggesting that these genes might be involved in xerotolerance. |
format | Online Article Text |
id | pubmed-8706201 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87062012021-12-25 Xerotolerance: A New Property in Exiguobacterium Genus López, María Castillo Galán, Beatriz Carmona, Manuel Navarro Llorens, Juana María Peretó, Juli Porcar, Manuel Getino, Luis Olivera, Elías R. Luengo, José M. Castro, Laura García, José Luís Microorganisms Article The highly xerotolerant bacterium classified as Exiguobacterium sp. Helios isolated from a solar panel in Spain showed a close relationship to Exiguobacterium sibiricum 255-15 isolated from Siberian permafrost. Xerotolerance has not been previously described as a characteristic of the extremely diverse Exiguobacterium genus, but both strains Helios and 255-15 showed higher xerotolerance than that described in the reference xerotolerant model strain Deinococcus radiodurans. Significant changes observed in the cell morphology after their desiccation suggests that the structure of cellular surface plays an important role in xerotolerance. Apart from its remarkable resistance to desiccation, Exiguobacterium sp. Helios strain shows several polyextremophilic characteristics that make it a promising chassis for biotechnological applications. Exiguobacterium sp. Helios cells produce nanoparticles of selenium in the presence of selenite linked to its resistance mechanism. Using the Lactobacillus plasmid pRCR12 that harbors a cherry marker, we have developed a transformation protocol for Exiguobacterium sp. Helios strain, being the first time that a bacterium of Exiguobacterium genus has been genetically modified. The comparison of Exiguobacterium sp. Helios and E. sibiricum 255-15 genomes revealed several interesting similarities and differences. Both strains contain a complete set of competence-related DNA transformation genes, suggesting that they might have natural competence, and an incomplete set of genes involved in sporulation; moreover, these strains not produce spores, suggesting that these genes might be involved in xerotolerance. MDPI 2021-11-28 /pmc/articles/PMC8706201/ /pubmed/34946057 http://dx.doi.org/10.3390/microorganisms9122455 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article López, María Castillo Galán, Beatriz Carmona, Manuel Navarro Llorens, Juana María Peretó, Juli Porcar, Manuel Getino, Luis Olivera, Elías R. Luengo, José M. Castro, Laura García, José Luís Xerotolerance: A New Property in Exiguobacterium Genus |
title | Xerotolerance: A New Property in Exiguobacterium Genus |
title_full | Xerotolerance: A New Property in Exiguobacterium Genus |
title_fullStr | Xerotolerance: A New Property in Exiguobacterium Genus |
title_full_unstemmed | Xerotolerance: A New Property in Exiguobacterium Genus |
title_short | Xerotolerance: A New Property in Exiguobacterium Genus |
title_sort | xerotolerance: a new property in exiguobacterium genus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706201/ https://www.ncbi.nlm.nih.gov/pubmed/34946057 http://dx.doi.org/10.3390/microorganisms9122455 |
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