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Volatile Organic Compounds, Indole, and Biogenic Amines Assessment in Two Mediterranean Irciniidae (Porifera, Demospongiae)

Solid phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS) was employed for the headspace determination of the volatile organic fraction emitted by two of the most common Mediterranean demosponges, Ircinia variabilis and Sarcotragus spinosulus, and of indole and some...

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Autores principales: Aresta, Antonella, Cotugno, Pietro, De Vietro, Nicoletta, Longo, Caterina, Mercurio, Maria, Ferriol, Pere, Zambonin, Carlo, Nonnis Marzano, Carlotta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706640/
https://www.ncbi.nlm.nih.gov/pubmed/34940710
http://dx.doi.org/10.3390/md19120711
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author Aresta, Antonella
Cotugno, Pietro
De Vietro, Nicoletta
Longo, Caterina
Mercurio, Maria
Ferriol, Pere
Zambonin, Carlo
Nonnis Marzano, Carlotta
author_facet Aresta, Antonella
Cotugno, Pietro
De Vietro, Nicoletta
Longo, Caterina
Mercurio, Maria
Ferriol, Pere
Zambonin, Carlo
Nonnis Marzano, Carlotta
author_sort Aresta, Antonella
collection PubMed
description Solid phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS) was employed for the headspace determination of the volatile organic fraction emitted by two of the most common Mediterranean demosponges, Ircinia variabilis and Sarcotragus spinosulus, and of indole and some biogenic amines released by sponges in an aqueous medium. A total of 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane and 75 µm carboxen/polydimethylsiloxane fibers were used for the headspace extraction of low molecular weight sulfur compounds from a hermetically sealed vial containing sponge fragments, while the direct immersion determination of indole and biogenic amines was performed. The biogenic amines were extracted after in-solution derivatization with isobutyl chloroformate. All analytical parameters (linearity, limits of detection, and quantification, precision, and recovery) were evaluated for indole and biogenic amines. SPME-GC-MS proved to be a reliable means of highlighting the differences between molecules released by different sponges, principally responsible for their smell. The combined approaches allowed the identification of several volatile compounds in the headspace and other molecules released by the sponges in an aqueous medium, including indole and the BAs cadaverine, histamine, isobutylamine, isopentylamine, propylamine, 2-phenylethylamine, putrescine and tryptamine. The results obtained represent a further contribution to the picture of odoriferous molecules secreted by sponges.
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spelling pubmed-87066402021-12-25 Volatile Organic Compounds, Indole, and Biogenic Amines Assessment in Two Mediterranean Irciniidae (Porifera, Demospongiae) Aresta, Antonella Cotugno, Pietro De Vietro, Nicoletta Longo, Caterina Mercurio, Maria Ferriol, Pere Zambonin, Carlo Nonnis Marzano, Carlotta Mar Drugs Article Solid phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS) was employed for the headspace determination of the volatile organic fraction emitted by two of the most common Mediterranean demosponges, Ircinia variabilis and Sarcotragus spinosulus, and of indole and some biogenic amines released by sponges in an aqueous medium. A total of 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane and 75 µm carboxen/polydimethylsiloxane fibers were used for the headspace extraction of low molecular weight sulfur compounds from a hermetically sealed vial containing sponge fragments, while the direct immersion determination of indole and biogenic amines was performed. The biogenic amines were extracted after in-solution derivatization with isobutyl chloroformate. All analytical parameters (linearity, limits of detection, and quantification, precision, and recovery) were evaluated for indole and biogenic amines. SPME-GC-MS proved to be a reliable means of highlighting the differences between molecules released by different sponges, principally responsible for their smell. The combined approaches allowed the identification of several volatile compounds in the headspace and other molecules released by the sponges in an aqueous medium, including indole and the BAs cadaverine, histamine, isobutylamine, isopentylamine, propylamine, 2-phenylethylamine, putrescine and tryptamine. The results obtained represent a further contribution to the picture of odoriferous molecules secreted by sponges. MDPI 2021-12-17 /pmc/articles/PMC8706640/ /pubmed/34940710 http://dx.doi.org/10.3390/md19120711 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aresta, Antonella
Cotugno, Pietro
De Vietro, Nicoletta
Longo, Caterina
Mercurio, Maria
Ferriol, Pere
Zambonin, Carlo
Nonnis Marzano, Carlotta
Volatile Organic Compounds, Indole, and Biogenic Amines Assessment in Two Mediterranean Irciniidae (Porifera, Demospongiae)
title Volatile Organic Compounds, Indole, and Biogenic Amines Assessment in Two Mediterranean Irciniidae (Porifera, Demospongiae)
title_full Volatile Organic Compounds, Indole, and Biogenic Amines Assessment in Two Mediterranean Irciniidae (Porifera, Demospongiae)
title_fullStr Volatile Organic Compounds, Indole, and Biogenic Amines Assessment in Two Mediterranean Irciniidae (Porifera, Demospongiae)
title_full_unstemmed Volatile Organic Compounds, Indole, and Biogenic Amines Assessment in Two Mediterranean Irciniidae (Porifera, Demospongiae)
title_short Volatile Organic Compounds, Indole, and Biogenic Amines Assessment in Two Mediterranean Irciniidae (Porifera, Demospongiae)
title_sort volatile organic compounds, indole, and biogenic amines assessment in two mediterranean irciniidae (porifera, demospongiae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706640/
https://www.ncbi.nlm.nih.gov/pubmed/34940710
http://dx.doi.org/10.3390/md19120711
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