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Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients

Objective: Long non-coding RNAs (lncRNAs) and their target microRNAs were documented in multiple studies to have a significant role in different joint disorders such as rheumatoid arthritis (RA) and osteoarthritis (OA). The current work aimed to determine the potential role of lnc-PVT1 and miR-146a...

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Autores principales: Erfan, Randa, Shaker, Olfat G., Khalil, Mahmoud A. F., Elsabagh, Yumn A., Ahmed, Azza M., Abu-El-Azayem, Abeer K., Gomaa, Mohamed S., Mohammed, Asmaa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706643/
https://www.ncbi.nlm.nih.gov/pubmed/34947913
http://dx.doi.org/10.3390/life11121382
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author Erfan, Randa
Shaker, Olfat G.
Khalil, Mahmoud A. F.
Elsabagh, Yumn A.
Ahmed, Azza M.
Abu-El-Azayem, Abeer K.
Gomaa, Mohamed S.
Mohammed, Asmaa
author_facet Erfan, Randa
Shaker, Olfat G.
Khalil, Mahmoud A. F.
Elsabagh, Yumn A.
Ahmed, Azza M.
Abu-El-Azayem, Abeer K.
Gomaa, Mohamed S.
Mohammed, Asmaa
author_sort Erfan, Randa
collection PubMed
description Objective: Long non-coding RNAs (lncRNAs) and their target microRNAs were documented in multiple studies to have a significant role in different joint disorders such as rheumatoid arthritis (RA) and osteoarthritis (OA). The current work aimed to determine the potential role of lnc-PVT1 and miR-146a as promising biomarkers to distinguish between RA, OA patients, and healthy individuals. Methods: The expression levels of lnc-PVT1 and its target miR-146a in the serum were measured for three different groups, including patients with RA (40), OA patients (40), and healthy controls (HCs) (40). Participating individuals were subjected to a full history investigation and clinical examination. Blood samples were tested for ESR, RF, CBC, as well as liver and renal functions. Serum was used to detect the relative expression levels of lnc-PVT1 and miR-146a and we correlated the levels with RA and OA activity and severity signs. Results: Lnc-PVT1 expression level was greater among patients with RA compared to that of OA patients, with a fold change median of 2.62 and 0.22, respectively (p = 0.001). The miR-146a fold change was significantly demonstrated between the RA, OA, and HCs groups. There was no correlation between both biomarkers with the disease activity scales (DAS28) of RA, the Knee injury Osteoarthritis Outcome Score (KOOS), or any sign of detection of the disease severity of OA. Conclusions: lnc-PVT1 and miR-146a could be considered as promising biomarkers for the diagnosis of RA and OA and may have an important role as therapeutic targets in the future.
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spelling pubmed-87066432021-12-25 Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients Erfan, Randa Shaker, Olfat G. Khalil, Mahmoud A. F. Elsabagh, Yumn A. Ahmed, Azza M. Abu-El-Azayem, Abeer K. Gomaa, Mohamed S. Mohammed, Asmaa Life (Basel) Article Objective: Long non-coding RNAs (lncRNAs) and their target microRNAs were documented in multiple studies to have a significant role in different joint disorders such as rheumatoid arthritis (RA) and osteoarthritis (OA). The current work aimed to determine the potential role of lnc-PVT1 and miR-146a as promising biomarkers to distinguish between RA, OA patients, and healthy individuals. Methods: The expression levels of lnc-PVT1 and its target miR-146a in the serum were measured for three different groups, including patients with RA (40), OA patients (40), and healthy controls (HCs) (40). Participating individuals were subjected to a full history investigation and clinical examination. Blood samples were tested for ESR, RF, CBC, as well as liver and renal functions. Serum was used to detect the relative expression levels of lnc-PVT1 and miR-146a and we correlated the levels with RA and OA activity and severity signs. Results: Lnc-PVT1 expression level was greater among patients with RA compared to that of OA patients, with a fold change median of 2.62 and 0.22, respectively (p = 0.001). The miR-146a fold change was significantly demonstrated between the RA, OA, and HCs groups. There was no correlation between both biomarkers with the disease activity scales (DAS28) of RA, the Knee injury Osteoarthritis Outcome Score (KOOS), or any sign of detection of the disease severity of OA. Conclusions: lnc-PVT1 and miR-146a could be considered as promising biomarkers for the diagnosis of RA and OA and may have an important role as therapeutic targets in the future. MDPI 2021-12-10 /pmc/articles/PMC8706643/ /pubmed/34947913 http://dx.doi.org/10.3390/life11121382 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Erfan, Randa
Shaker, Olfat G.
Khalil, Mahmoud A. F.
Elsabagh, Yumn A.
Ahmed, Azza M.
Abu-El-Azayem, Abeer K.
Gomaa, Mohamed S.
Mohammed, Asmaa
Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients
title Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients
title_full Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients
title_fullStr Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients
title_full_unstemmed Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients
title_short Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients
title_sort long non-coding rna pvt1 and its target mirna-146a as potential prognostic biomarkers in rheumatoid arthritis patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706643/
https://www.ncbi.nlm.nih.gov/pubmed/34947913
http://dx.doi.org/10.3390/life11121382
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