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Acute Effect of Caffeine on the Synthesis of Pro-Inflammatory Cytokines in the Hypothalamus and Choroid Plexus during Endotoxin-Induced Inflammation in a Female Sheep Model

This study was designed to determine the effect of acute caffeine (CAF) administration, which exerts a broad spectrum of anti-inflammatory activity, on the synthesis of pro-inflammatory cytokines and their receptors in the hypothalamus and choroid plexus (ChP) during acute inflammation caused by the...

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Autores principales: Szczepkowska, Aleksandra, Wójcik, Maciej, Tomaszewska-Zaremba, Dorota, Antushevich, Hanna, Krawczyńska, Agata, Wiechetek, Wiktoria, Skipor, Janina, Herman, Andrzej Przemysław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706723/
https://www.ncbi.nlm.nih.gov/pubmed/34948033
http://dx.doi.org/10.3390/ijms222413237
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author Szczepkowska, Aleksandra
Wójcik, Maciej
Tomaszewska-Zaremba, Dorota
Antushevich, Hanna
Krawczyńska, Agata
Wiechetek, Wiktoria
Skipor, Janina
Herman, Andrzej Przemysław
author_facet Szczepkowska, Aleksandra
Wójcik, Maciej
Tomaszewska-Zaremba, Dorota
Antushevich, Hanna
Krawczyńska, Agata
Wiechetek, Wiktoria
Skipor, Janina
Herman, Andrzej Przemysław
author_sort Szczepkowska, Aleksandra
collection PubMed
description This study was designed to determine the effect of acute caffeine (CAF) administration, which exerts a broad spectrum of anti-inflammatory activity, on the synthesis of pro-inflammatory cytokines and their receptors in the hypothalamus and choroid plexus (ChP) during acute inflammation caused by the injection of bacterial endotoxin—lipopolysaccharide (LPS). The experiment was performed on 24 female sheep randomly divided into four groups: control; LPS treated (iv.; 400 ng/kg of body mass (bm.)); CAF treated (iv.; 30 mg/kg of bm.); and LPS and CAF treated. The animals were euthanized 3 h after the treatment. It was found that acute administration of CAF suppressed the synthesis of interleukin (IL-1β) and tumor necrosis factor (TNF)α, but did not influence IL-6, in the hypothalamus during LPS-induced inflammation. The injection of CAF reduced the LPS-induced expression of TNF mRNA in the ChP. CAF lowered the gene expression of IL-6 cytokine family signal transducer (IL6ST) and TNF receptor superfamily member 1A (TNFRSF1) in the hypothalamus and IL-1 type II receptor (IL1R2) in the ChP. Our study on the sheep model suggests that CAF may attenuate the inflammatory response at the hypothalamic level and partly influence the inflammatory signal generated by the ChP cells. This suggests the potential of CAF to suppress neuroinflammatory processes induced by peripheral immune/inflammatory challenges.
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spelling pubmed-87067232021-12-25 Acute Effect of Caffeine on the Synthesis of Pro-Inflammatory Cytokines in the Hypothalamus and Choroid Plexus during Endotoxin-Induced Inflammation in a Female Sheep Model Szczepkowska, Aleksandra Wójcik, Maciej Tomaszewska-Zaremba, Dorota Antushevich, Hanna Krawczyńska, Agata Wiechetek, Wiktoria Skipor, Janina Herman, Andrzej Przemysław Int J Mol Sci Article This study was designed to determine the effect of acute caffeine (CAF) administration, which exerts a broad spectrum of anti-inflammatory activity, on the synthesis of pro-inflammatory cytokines and their receptors in the hypothalamus and choroid plexus (ChP) during acute inflammation caused by the injection of bacterial endotoxin—lipopolysaccharide (LPS). The experiment was performed on 24 female sheep randomly divided into four groups: control; LPS treated (iv.; 400 ng/kg of body mass (bm.)); CAF treated (iv.; 30 mg/kg of bm.); and LPS and CAF treated. The animals were euthanized 3 h after the treatment. It was found that acute administration of CAF suppressed the synthesis of interleukin (IL-1β) and tumor necrosis factor (TNF)α, but did not influence IL-6, in the hypothalamus during LPS-induced inflammation. The injection of CAF reduced the LPS-induced expression of TNF mRNA in the ChP. CAF lowered the gene expression of IL-6 cytokine family signal transducer (IL6ST) and TNF receptor superfamily member 1A (TNFRSF1) in the hypothalamus and IL-1 type II receptor (IL1R2) in the ChP. Our study on the sheep model suggests that CAF may attenuate the inflammatory response at the hypothalamic level and partly influence the inflammatory signal generated by the ChP cells. This suggests the potential of CAF to suppress neuroinflammatory processes induced by peripheral immune/inflammatory challenges. MDPI 2021-12-08 /pmc/articles/PMC8706723/ /pubmed/34948033 http://dx.doi.org/10.3390/ijms222413237 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szczepkowska, Aleksandra
Wójcik, Maciej
Tomaszewska-Zaremba, Dorota
Antushevich, Hanna
Krawczyńska, Agata
Wiechetek, Wiktoria
Skipor, Janina
Herman, Andrzej Przemysław
Acute Effect of Caffeine on the Synthesis of Pro-Inflammatory Cytokines in the Hypothalamus and Choroid Plexus during Endotoxin-Induced Inflammation in a Female Sheep Model
title Acute Effect of Caffeine on the Synthesis of Pro-Inflammatory Cytokines in the Hypothalamus and Choroid Plexus during Endotoxin-Induced Inflammation in a Female Sheep Model
title_full Acute Effect of Caffeine on the Synthesis of Pro-Inflammatory Cytokines in the Hypothalamus and Choroid Plexus during Endotoxin-Induced Inflammation in a Female Sheep Model
title_fullStr Acute Effect of Caffeine on the Synthesis of Pro-Inflammatory Cytokines in the Hypothalamus and Choroid Plexus during Endotoxin-Induced Inflammation in a Female Sheep Model
title_full_unstemmed Acute Effect of Caffeine on the Synthesis of Pro-Inflammatory Cytokines in the Hypothalamus and Choroid Plexus during Endotoxin-Induced Inflammation in a Female Sheep Model
title_short Acute Effect of Caffeine on the Synthesis of Pro-Inflammatory Cytokines in the Hypothalamus and Choroid Plexus during Endotoxin-Induced Inflammation in a Female Sheep Model
title_sort acute effect of caffeine on the synthesis of pro-inflammatory cytokines in the hypothalamus and choroid plexus during endotoxin-induced inflammation in a female sheep model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706723/
https://www.ncbi.nlm.nih.gov/pubmed/34948033
http://dx.doi.org/10.3390/ijms222413237
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