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Altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth
The metabolic reprogramming of phospholipids may affect intracellular signal transduction pathways. A high‐fat diet (HFD) is attributed to prostate cancer (PCa) progression, but the expression pattern and role of phospholipids in HFD‐mediated PCa progression remains unclear. In this study, HFD enhan...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706770/ https://www.ncbi.nlm.nih.gov/pubmed/34977875 http://dx.doi.org/10.1002/mco2.89 |
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author | Huang, Mingguo Koizumi, Atsushi Narita, Shintaro Nakanishi, Hiroki Sato, Hiromi Kashima, Soki Nara, Taketoshi Kanda, Sohei Numakura, Kazuyuki Saito, Mitsuru Satoh, Shigeru Nanjo, Hiroshi Sasaki, Takehiko Habuchi, Tomonori |
author_facet | Huang, Mingguo Koizumi, Atsushi Narita, Shintaro Nakanishi, Hiroki Sato, Hiromi Kashima, Soki Nara, Taketoshi Kanda, Sohei Numakura, Kazuyuki Saito, Mitsuru Satoh, Shigeru Nanjo, Hiroshi Sasaki, Takehiko Habuchi, Tomonori |
author_sort | Huang, Mingguo |
collection | PubMed |
description | The metabolic reprogramming of phospholipids may affect intracellular signal transduction pathways. A high‐fat diet (HFD) is attributed to prostate cancer (PCa) progression, but the expression pattern and role of phospholipids in HFD‐mediated PCa progression remains unclear. In this study, HFD enhanced LNCaP xenograft tumor growth by upregulating the phosphatidylinositol (PI) 3‐kinase (PI3K)/AKT signaling pathway. A lipidomic analysis using xenograft tumors showed that phosphoinositides, especially PI (3,4,5)‐trisphosphate (PIP(3)), including several species containing C38:4, C38:3, and C40:4 fatty acids, increased in the HFD group compared to control. Fatty acid synthase (FASN) was significantly upregulated in xenograft tumors under HFD in both gene and protein levels. PCa cell growth was significantly inhibited through the decreased AKT signaling pathway by treatment with cerulenin, a chemical FASN inhibitor, which also downregulated PIP, PIP(2), and PIP(3) but not PI. Thus, dietary fat influences PCa progression and alters phosphoinositides, especially PIP(3), a critical player in the PI3K/AKT pathway. These results may offer appropriate targets, such as FASN, for dietary intervention and/or chemoprevention to reduce PCa incidence and progression. |
format | Online Article Text |
id | pubmed-8706770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87067702021-12-30 Altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth Huang, Mingguo Koizumi, Atsushi Narita, Shintaro Nakanishi, Hiroki Sato, Hiromi Kashima, Soki Nara, Taketoshi Kanda, Sohei Numakura, Kazuyuki Saito, Mitsuru Satoh, Shigeru Nanjo, Hiroshi Sasaki, Takehiko Habuchi, Tomonori MedComm (2020) Original Articles The metabolic reprogramming of phospholipids may affect intracellular signal transduction pathways. A high‐fat diet (HFD) is attributed to prostate cancer (PCa) progression, but the expression pattern and role of phospholipids in HFD‐mediated PCa progression remains unclear. In this study, HFD enhanced LNCaP xenograft tumor growth by upregulating the phosphatidylinositol (PI) 3‐kinase (PI3K)/AKT signaling pathway. A lipidomic analysis using xenograft tumors showed that phosphoinositides, especially PI (3,4,5)‐trisphosphate (PIP(3)), including several species containing C38:4, C38:3, and C40:4 fatty acids, increased in the HFD group compared to control. Fatty acid synthase (FASN) was significantly upregulated in xenograft tumors under HFD in both gene and protein levels. PCa cell growth was significantly inhibited through the decreased AKT signaling pathway by treatment with cerulenin, a chemical FASN inhibitor, which also downregulated PIP, PIP(2), and PIP(3) but not PI. Thus, dietary fat influences PCa progression and alters phosphoinositides, especially PIP(3), a critical player in the PI3K/AKT pathway. These results may offer appropriate targets, such as FASN, for dietary intervention and/or chemoprevention to reduce PCa incidence and progression. John Wiley and Sons Inc. 2021-10-28 /pmc/articles/PMC8706770/ /pubmed/34977875 http://dx.doi.org/10.1002/mco2.89 Text en © 2021 The Authors. MedComm published by Sichuan International Medical Exchange & Promotion Association (SCIMEA) and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Huang, Mingguo Koizumi, Atsushi Narita, Shintaro Nakanishi, Hiroki Sato, Hiromi Kashima, Soki Nara, Taketoshi Kanda, Sohei Numakura, Kazuyuki Saito, Mitsuru Satoh, Shigeru Nanjo, Hiroshi Sasaki, Takehiko Habuchi, Tomonori Altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth |
title | Altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth |
title_full | Altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth |
title_fullStr | Altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth |
title_full_unstemmed | Altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth |
title_short | Altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth |
title_sort | altering phosphoinositides in high‐fat diet‐associated prostate tumor xenograft growth |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706770/ https://www.ncbi.nlm.nih.gov/pubmed/34977875 http://dx.doi.org/10.1002/mco2.89 |
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