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Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes
Fumonisin B(1) (FB(1)), as the most prevalent and toxic fumonisin, poses a health threat to humans and animals. The cytotoxicity of FB(1) is closely related to oxidative stress and apoptosis. The purpose of this study is to explore whether Grape seed proanthocyanidin (GSP), a natural antioxidant, co...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706835/ https://www.ncbi.nlm.nih.gov/pubmed/34941679 http://dx.doi.org/10.3390/toxins13120841 |
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author | Li, Wenhui He, Yijing Zhao, Hongyu Peng, Lei Li, Jia Rui, Rong Ju, Shiqiang |
author_facet | Li, Wenhui He, Yijing Zhao, Hongyu Peng, Lei Li, Jia Rui, Rong Ju, Shiqiang |
author_sort | Li, Wenhui |
collection | PubMed |
description | Fumonisin B(1) (FB(1)), as the most prevalent and toxic fumonisin, poses a health threat to humans and animals. The cytotoxicity of FB(1) is closely related to oxidative stress and apoptosis. The purpose of this study is to explore whether Grape seed proanthocyanidin (GSP), a natural antioxidant, could alleviate the meiotic maturation defects of oocytes caused by FB(1) exposure. Porcine cumulus oocyte complexes (COCs) were treated with 30 μM FB(1) alone or cotreated with 100, 200 and 300 μM GSP during in vitro maturation for 44 h. The results show that 200 μM GSP cotreatment observably ameliorated the toxic effects of FB(1) exposure, showing to be promoting first polar body extrusion and improving the subsequent cleavage rate and blastocyst development rate. Moreover, 200 μM GSP cotreatment restored cell cycle progression, reduced the proportion of aberrant spindles, improved actin distribution and protected mitochondrial function in FB(1)-exposed oocytes. Furthermore, reactive oxygen species (ROS) generation was significantly decreased and the mRNA levels of CAT, SOD2 and GSH-PX were obviously increased in the 200 μM GSP cotreatment group. Notably, the incidence of early apoptosis and autophagy level were also significantly decreased after GSP cotreatment and the mRNA expression levels of BAX, CASPASE3, LC3 and ATG5 were markedly decreased, whereas BCL2 and mTOR were observably increased in the oocytes after GSP cotreatment. Together, these results indicate that GSP could exert significant preventive effects on FB(1)-induced oocyte defects by ameliorating oxidative stress through repairing mitochondrial dysfunction. |
format | Online Article Text |
id | pubmed-8706835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87068352021-12-25 Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes Li, Wenhui He, Yijing Zhao, Hongyu Peng, Lei Li, Jia Rui, Rong Ju, Shiqiang Toxins (Basel) Article Fumonisin B(1) (FB(1)), as the most prevalent and toxic fumonisin, poses a health threat to humans and animals. The cytotoxicity of FB(1) is closely related to oxidative stress and apoptosis. The purpose of this study is to explore whether Grape seed proanthocyanidin (GSP), a natural antioxidant, could alleviate the meiotic maturation defects of oocytes caused by FB(1) exposure. Porcine cumulus oocyte complexes (COCs) were treated with 30 μM FB(1) alone or cotreated with 100, 200 and 300 μM GSP during in vitro maturation for 44 h. The results show that 200 μM GSP cotreatment observably ameliorated the toxic effects of FB(1) exposure, showing to be promoting first polar body extrusion and improving the subsequent cleavage rate and blastocyst development rate. Moreover, 200 μM GSP cotreatment restored cell cycle progression, reduced the proportion of aberrant spindles, improved actin distribution and protected mitochondrial function in FB(1)-exposed oocytes. Furthermore, reactive oxygen species (ROS) generation was significantly decreased and the mRNA levels of CAT, SOD2 and GSH-PX were obviously increased in the 200 μM GSP cotreatment group. Notably, the incidence of early apoptosis and autophagy level were also significantly decreased after GSP cotreatment and the mRNA expression levels of BAX, CASPASE3, LC3 and ATG5 were markedly decreased, whereas BCL2 and mTOR were observably increased in the oocytes after GSP cotreatment. Together, these results indicate that GSP could exert significant preventive effects on FB(1)-induced oocyte defects by ameliorating oxidative stress through repairing mitochondrial dysfunction. MDPI 2021-11-25 /pmc/articles/PMC8706835/ /pubmed/34941679 http://dx.doi.org/10.3390/toxins13120841 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Wenhui He, Yijing Zhao, Hongyu Peng, Lei Li, Jia Rui, Rong Ju, Shiqiang Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes |
title | Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes |
title_full | Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes |
title_fullStr | Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes |
title_full_unstemmed | Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes |
title_short | Grape Seed Proanthocyanidin Ameliorates FB(1)-Induced Meiotic Defects in Porcine Oocytes |
title_sort | grape seed proanthocyanidin ameliorates fb(1)-induced meiotic defects in porcine oocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706835/ https://www.ncbi.nlm.nih.gov/pubmed/34941679 http://dx.doi.org/10.3390/toxins13120841 |
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