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An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil
Newcastle disease virus (NDV) can infect over 250 bird species with variable pathogenicity; it can also infect humans in rare cases. The present study investigated an outbreak in feral pigeons in São Paulo city, Brazil, in 2019. Affected birds displayed neurological signs, and hemorrhages were obser...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706928/ https://www.ncbi.nlm.nih.gov/pubmed/34960715 http://dx.doi.org/10.3390/v13122446 |
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author | Thomazelli, Luciano M. Sinhorini, Juliana A. Oliveira, Danielle B. L. Knöbl, Terezinha Bosqueiro, Tatiana C. M. Sano, Elder Costa, Gladyston C. V. Monteiro, Cairo Dorlass, Erick G. Utecht, Nathalia Scagion, Guilherme P. Meneguin, Carla Silva, Laura M. N. Moraes, Maria Vitória S. Bueno, Larissa M. Reischak, Dilmara Carrasco, Adriano O. T. Arns, Clarice W. Ferreira, Helena L. Durigon, Edison L. |
author_facet | Thomazelli, Luciano M. Sinhorini, Juliana A. Oliveira, Danielle B. L. Knöbl, Terezinha Bosqueiro, Tatiana C. M. Sano, Elder Costa, Gladyston C. V. Monteiro, Cairo Dorlass, Erick G. Utecht, Nathalia Scagion, Guilherme P. Meneguin, Carla Silva, Laura M. N. Moraes, Maria Vitória S. Bueno, Larissa M. Reischak, Dilmara Carrasco, Adriano O. T. Arns, Clarice W. Ferreira, Helena L. Durigon, Edison L. |
author_sort | Thomazelli, Luciano M. |
collection | PubMed |
description | Newcastle disease virus (NDV) can infect over 250 bird species with variable pathogenicity; it can also infect humans in rare cases. The present study investigated an outbreak in feral pigeons in São Paulo city, Brazil, in 2019. Affected birds displayed neurological signs, and hemorrhages were observed in different tissues. Histopathology changes with infiltration of mononuclear inflammatory cells were also found in the brain, kidney, proventriculus, heart, and spleen. NDV staining was detected by immunohistochemistry. Twenty-seven out of thirty-four tested samples (swabs and tissues) were positive for Newcastle disease virus by RT-qPCR test, targeting the M gene. One isolate, obtained from a pool of positive swab samples, was characterized by the intracerebral pathogenicity index (ICPI) and the hemagglutination inhibition (HI) tests. This isolate had an ICPI of 0.99, confirming a virulent NDV strain. The monoclonal antibody 617/161, which recognizes a distinct epitope in pigeon NDV strains, inhibited the isolate with an HI titer of 512. A complete genome of NDV was obtained using next-generation sequencing. Phylogenetic analysis based on the complete CDS F gene grouped the detected isolate with other viruses from subgenotype VI.2.1.2, class II, including one previously reported in Southern Brazil in 2014. This study reports a comprehensive characterization of the subgenotype VI.2.1.2, which seems to have been circulating in Brazilian urban areas since 2014. Due to the zoonotic risk of NDV, virus surveillance in feral pigeons should also be systematically performed in urban areas. |
format | Online Article Text |
id | pubmed-8706928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87069282021-12-25 An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil Thomazelli, Luciano M. Sinhorini, Juliana A. Oliveira, Danielle B. L. Knöbl, Terezinha Bosqueiro, Tatiana C. M. Sano, Elder Costa, Gladyston C. V. Monteiro, Cairo Dorlass, Erick G. Utecht, Nathalia Scagion, Guilherme P. Meneguin, Carla Silva, Laura M. N. Moraes, Maria Vitória S. Bueno, Larissa M. Reischak, Dilmara Carrasco, Adriano O. T. Arns, Clarice W. Ferreira, Helena L. Durigon, Edison L. Viruses Case Report Newcastle disease virus (NDV) can infect over 250 bird species with variable pathogenicity; it can also infect humans in rare cases. The present study investigated an outbreak in feral pigeons in São Paulo city, Brazil, in 2019. Affected birds displayed neurological signs, and hemorrhages were observed in different tissues. Histopathology changes with infiltration of mononuclear inflammatory cells were also found in the brain, kidney, proventriculus, heart, and spleen. NDV staining was detected by immunohistochemistry. Twenty-seven out of thirty-four tested samples (swabs and tissues) were positive for Newcastle disease virus by RT-qPCR test, targeting the M gene. One isolate, obtained from a pool of positive swab samples, was characterized by the intracerebral pathogenicity index (ICPI) and the hemagglutination inhibition (HI) tests. This isolate had an ICPI of 0.99, confirming a virulent NDV strain. The monoclonal antibody 617/161, which recognizes a distinct epitope in pigeon NDV strains, inhibited the isolate with an HI titer of 512. A complete genome of NDV was obtained using next-generation sequencing. Phylogenetic analysis based on the complete CDS F gene grouped the detected isolate with other viruses from subgenotype VI.2.1.2, class II, including one previously reported in Southern Brazil in 2014. This study reports a comprehensive characterization of the subgenotype VI.2.1.2, which seems to have been circulating in Brazilian urban areas since 2014. Due to the zoonotic risk of NDV, virus surveillance in feral pigeons should also be systematically performed in urban areas. MDPI 2021-12-06 /pmc/articles/PMC8706928/ /pubmed/34960715 http://dx.doi.org/10.3390/v13122446 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Case Report Thomazelli, Luciano M. Sinhorini, Juliana A. Oliveira, Danielle B. L. Knöbl, Terezinha Bosqueiro, Tatiana C. M. Sano, Elder Costa, Gladyston C. V. Monteiro, Cairo Dorlass, Erick G. Utecht, Nathalia Scagion, Guilherme P. Meneguin, Carla Silva, Laura M. N. Moraes, Maria Vitória S. Bueno, Larissa M. Reischak, Dilmara Carrasco, Adriano O. T. Arns, Clarice W. Ferreira, Helena L. Durigon, Edison L. An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil |
title | An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil |
title_full | An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil |
title_fullStr | An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil |
title_full_unstemmed | An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil |
title_short | An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil |
title_sort | outbreak in pigeons caused by the subgenotype vi.2.1.2 of newcastle disease virus in brazil |
topic | Case Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706928/ https://www.ncbi.nlm.nih.gov/pubmed/34960715 http://dx.doi.org/10.3390/v13122446 |
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