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Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli

Wounding stress is an effective strategy to induce glucosinolate (GS) biosynthesis in broccoli. However, there is insufficient knowledge on the physiological and molecular mechanisms underlying this stress response. Herein, a chemical-genetic approach was applied to elucidate the role of jasmonic ac...

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Autores principales: Torres-Contreras, Ana M., Nair, Vimal, Senés-Guerrero, Carolina, Pacheco, Adriana, González-Agüero, Mauricio, Ramos-Parra, Perla A., Cisneros-Zevallos, Luis, Jacobo-Velázquez, Daniel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706940/
https://www.ncbi.nlm.nih.gov/pubmed/34961133
http://dx.doi.org/10.3390/plants10122660
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author Torres-Contreras, Ana M.
Nair, Vimal
Senés-Guerrero, Carolina
Pacheco, Adriana
González-Agüero, Mauricio
Ramos-Parra, Perla A.
Cisneros-Zevallos, Luis
Jacobo-Velázquez, Daniel A.
author_facet Torres-Contreras, Ana M.
Nair, Vimal
Senés-Guerrero, Carolina
Pacheco, Adriana
González-Agüero, Mauricio
Ramos-Parra, Perla A.
Cisneros-Zevallos, Luis
Jacobo-Velázquez, Daniel A.
author_sort Torres-Contreras, Ana M.
collection PubMed
description Wounding stress is an effective strategy to induce glucosinolate (GS) biosynthesis in broccoli. However, there is insufficient knowledge on the physiological and molecular mechanisms underlying this stress response. Herein, a chemical-genetic approach was applied to elucidate the role of jasmonic acid (JA), ethylene (ET), and reactive oxygen species (ROS) on the wound-induced biosynthesis of GS. Broccoli was processed into chops to induce wounding stress. Broccoli chops were treated with phenidone (PHEN) and diphenyleneiodonium chloride (DPI) as inhibitors of JA and ROS biosynthesis, respectively, whereas 1-methylcyclopropene (1-MCP) was applied as an inhibitor of ET action. Wounding stress induced the expression of genes related to the biosynthesis of indolic and aliphatic GS, which was correlated with the accumulation of GS and modulated by the inhibitors of signaling molecules applied. Results of gene expression analysis indicated that JA played a key role in the activation of most genes, followed by ROS. Furthermore, except for the CYP79B2 gene, PHEN and 1-MCP synergistically downregulated the expression of GS biosynthetic genes evaluated, showing that the interaction between JA and ET was fundamental to modulate GS biosynthesis. Results presented herein increased our knowledge of the physiological and molecular mechanisms governing the wound-induced biosynthesis of GS in broccoli.
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spelling pubmed-87069402021-12-25 Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli Torres-Contreras, Ana M. Nair, Vimal Senés-Guerrero, Carolina Pacheco, Adriana González-Agüero, Mauricio Ramos-Parra, Perla A. Cisneros-Zevallos, Luis Jacobo-Velázquez, Daniel A. Plants (Basel) Article Wounding stress is an effective strategy to induce glucosinolate (GS) biosynthesis in broccoli. However, there is insufficient knowledge on the physiological and molecular mechanisms underlying this stress response. Herein, a chemical-genetic approach was applied to elucidate the role of jasmonic acid (JA), ethylene (ET), and reactive oxygen species (ROS) on the wound-induced biosynthesis of GS. Broccoli was processed into chops to induce wounding stress. Broccoli chops were treated with phenidone (PHEN) and diphenyleneiodonium chloride (DPI) as inhibitors of JA and ROS biosynthesis, respectively, whereas 1-methylcyclopropene (1-MCP) was applied as an inhibitor of ET action. Wounding stress induced the expression of genes related to the biosynthesis of indolic and aliphatic GS, which was correlated with the accumulation of GS and modulated by the inhibitors of signaling molecules applied. Results of gene expression analysis indicated that JA played a key role in the activation of most genes, followed by ROS. Furthermore, except for the CYP79B2 gene, PHEN and 1-MCP synergistically downregulated the expression of GS biosynthetic genes evaluated, showing that the interaction between JA and ET was fundamental to modulate GS biosynthesis. Results presented herein increased our knowledge of the physiological and molecular mechanisms governing the wound-induced biosynthesis of GS in broccoli. MDPI 2021-12-03 /pmc/articles/PMC8706940/ /pubmed/34961133 http://dx.doi.org/10.3390/plants10122660 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Torres-Contreras, Ana M.
Nair, Vimal
Senés-Guerrero, Carolina
Pacheco, Adriana
González-Agüero, Mauricio
Ramos-Parra, Perla A.
Cisneros-Zevallos, Luis
Jacobo-Velázquez, Daniel A.
Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli
title Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli
title_full Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli
title_fullStr Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli
title_full_unstemmed Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli
title_short Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli
title_sort chemical genetics applied to elucidate the physiological role of stress-signaling molecules on the wound-induced accumulation of glucosinolates in broccoli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706940/
https://www.ncbi.nlm.nih.gov/pubmed/34961133
http://dx.doi.org/10.3390/plants10122660
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