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Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings

The global economic success of man-made nanoscale materials has led to a higher production rate and diversification of emission sources in the environment. For these reasons, novel nanosafety approaches to assess the environmental impact of engineered nanomaterials are required. While studying the p...

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Autores principales: Ferrari, Eleonora, Barbero, Francesco, Busquets-Fité, Marti, Franz-Wachtel, Mirita, Köhler, Heinz-R., Puntes, Victor, Kemmerling, Birgit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707008/
https://www.ncbi.nlm.nih.gov/pubmed/34947510
http://dx.doi.org/10.3390/nano11123161
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author Ferrari, Eleonora
Barbero, Francesco
Busquets-Fité, Marti
Franz-Wachtel, Mirita
Köhler, Heinz-R.
Puntes, Victor
Kemmerling, Birgit
author_facet Ferrari, Eleonora
Barbero, Francesco
Busquets-Fité, Marti
Franz-Wachtel, Mirita
Köhler, Heinz-R.
Puntes, Victor
Kemmerling, Birgit
author_sort Ferrari, Eleonora
collection PubMed
description The global economic success of man-made nanoscale materials has led to a higher production rate and diversification of emission sources in the environment. For these reasons, novel nanosafety approaches to assess the environmental impact of engineered nanomaterials are required. While studying the potential toxicity of metal nanoparticles (NPs), we realized that gold nanoparticles (AuNPs) have a growth-promoting rather than a stress-inducing effect. In this study we established stable short- and long-term exposition systems for testing plant responses to NPs. Exposure of plants to moderate concentrations of AuNPs resulted in enhanced growth of the plants with longer primary roots, more and longer lateral roots and increased rosette diameter, and reduced oxidative stress responses elicited by the immune-stimulatory PAMP flg22. Our data did not reveal any detrimental effects of AuNPs on plants but clearly showed positive effects on growth, presumably by their protective influence on oxidative stress responses. Differential transcriptomics and proteomics analyses revealed that oxidative stress responses are downregulated whereas growth-promoting genes/proteins are upregulated. These omics datasets after AuNP exposure can now be exploited to study the underlying molecular mechanisms of AuNP-induced growth-promotion.
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spelling pubmed-87070082021-12-25 Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings Ferrari, Eleonora Barbero, Francesco Busquets-Fité, Marti Franz-Wachtel, Mirita Köhler, Heinz-R. Puntes, Victor Kemmerling, Birgit Nanomaterials (Basel) Article The global economic success of man-made nanoscale materials has led to a higher production rate and diversification of emission sources in the environment. For these reasons, novel nanosafety approaches to assess the environmental impact of engineered nanomaterials are required. While studying the potential toxicity of metal nanoparticles (NPs), we realized that gold nanoparticles (AuNPs) have a growth-promoting rather than a stress-inducing effect. In this study we established stable short- and long-term exposition systems for testing plant responses to NPs. Exposure of plants to moderate concentrations of AuNPs resulted in enhanced growth of the plants with longer primary roots, more and longer lateral roots and increased rosette diameter, and reduced oxidative stress responses elicited by the immune-stimulatory PAMP flg22. Our data did not reveal any detrimental effects of AuNPs on plants but clearly showed positive effects on growth, presumably by their protective influence on oxidative stress responses. Differential transcriptomics and proteomics analyses revealed that oxidative stress responses are downregulated whereas growth-promoting genes/proteins are upregulated. These omics datasets after AuNP exposure can now be exploited to study the underlying molecular mechanisms of AuNP-induced growth-promotion. MDPI 2021-11-23 /pmc/articles/PMC8707008/ /pubmed/34947510 http://dx.doi.org/10.3390/nano11123161 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferrari, Eleonora
Barbero, Francesco
Busquets-Fité, Marti
Franz-Wachtel, Mirita
Köhler, Heinz-R.
Puntes, Victor
Kemmerling, Birgit
Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings
title Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings
title_full Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings
title_fullStr Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings
title_full_unstemmed Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings
title_short Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings
title_sort growth-promoting gold nanoparticles decrease stress responses in arabidopsis seedlings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707008/
https://www.ncbi.nlm.nih.gov/pubmed/34947510
http://dx.doi.org/10.3390/nano11123161
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