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Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling

We previously reported that Lepechinia meyenii (Walp.) Epling has antioxidant and aldose reductase (AR) inhibitory activities. In this study, L. meyenii was extracted in a 50% MeOH and CH(2)Cl(2)/MeOH system. The active extracts of MeOH and 50% MeOH were subjected to fractionation, followed by separ...

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Autores principales: Zuo, Guanglei, Je, Kang-Hoon, Guillen Quispe, Yanymee N., Shin, Kyong-Oh, Kim, Hyun Yong, Kim, Kang Hyuk, Arce, Paul H. Gonzales, Lim, Soon Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707451/
https://www.ncbi.nlm.nih.gov/pubmed/34961244
http://dx.doi.org/10.3390/plants10122773
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author Zuo, Guanglei
Je, Kang-Hoon
Guillen Quispe, Yanymee N.
Shin, Kyong-Oh
Kim, Hyun Yong
Kim, Kang Hyuk
Arce, Paul H. Gonzales
Lim, Soon Sung
author_facet Zuo, Guanglei
Je, Kang-Hoon
Guillen Quispe, Yanymee N.
Shin, Kyong-Oh
Kim, Hyun Yong
Kim, Kang Hyuk
Arce, Paul H. Gonzales
Lim, Soon Sung
author_sort Zuo, Guanglei
collection PubMed
description We previously reported that Lepechinia meyenii (Walp.) Epling has antioxidant and aldose reductase (AR) inhibitory activities. In this study, L. meyenii was extracted in a 50% MeOH and CH(2)Cl(2)/MeOH system. The active extracts of MeOH and 50% MeOH were subjected to fractionation, followed by separation using high-speed counter-current chromatography (HSCCC) and preparative HPLC. Separation and identification revealed the presence of caffeic acid, hesperidin, rosmarinic acid, diosmin, methyl rosmarinate, diosmetin, and butyl rosmarinate. Of these, rosmarinic acid, methyl rosmarinate, and butyl rosmarinate possessed remarkable antioxidant and AR inhibitory activities. The other compounds were less active. In particular, rosmarinic acid is the key contributor to the antioxidant and AR inhibitory activities of L. meyenii; it is rich in the MeOH extract (333.84 mg/g) and 50% MeOH extract (135.41 mg/g) of L. meyenii and is especially abundant in the EtOAc and n-BuOH fractions (373.71–804.07 mg/g) of the MeOH and 50% MeOH extracts. The results clarified the basis of antioxidant and AR inhibitory activity of L. meyenii, adding scientific evidence supporting its traditional use as an anti-diabetic herbal medicine. The HSCCC separation method established in this study can be used for the preparative separation of rosmarinic acid from natural products.
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spelling pubmed-87074512021-12-25 Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling Zuo, Guanglei Je, Kang-Hoon Guillen Quispe, Yanymee N. Shin, Kyong-Oh Kim, Hyun Yong Kim, Kang Hyuk Arce, Paul H. Gonzales Lim, Soon Sung Plants (Basel) Article We previously reported that Lepechinia meyenii (Walp.) Epling has antioxidant and aldose reductase (AR) inhibitory activities. In this study, L. meyenii was extracted in a 50% MeOH and CH(2)Cl(2)/MeOH system. The active extracts of MeOH and 50% MeOH were subjected to fractionation, followed by separation using high-speed counter-current chromatography (HSCCC) and preparative HPLC. Separation and identification revealed the presence of caffeic acid, hesperidin, rosmarinic acid, diosmin, methyl rosmarinate, diosmetin, and butyl rosmarinate. Of these, rosmarinic acid, methyl rosmarinate, and butyl rosmarinate possessed remarkable antioxidant and AR inhibitory activities. The other compounds were less active. In particular, rosmarinic acid is the key contributor to the antioxidant and AR inhibitory activities of L. meyenii; it is rich in the MeOH extract (333.84 mg/g) and 50% MeOH extract (135.41 mg/g) of L. meyenii and is especially abundant in the EtOAc and n-BuOH fractions (373.71–804.07 mg/g) of the MeOH and 50% MeOH extracts. The results clarified the basis of antioxidant and AR inhibitory activity of L. meyenii, adding scientific evidence supporting its traditional use as an anti-diabetic herbal medicine. The HSCCC separation method established in this study can be used for the preparative separation of rosmarinic acid from natural products. MDPI 2021-12-15 /pmc/articles/PMC8707451/ /pubmed/34961244 http://dx.doi.org/10.3390/plants10122773 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zuo, Guanglei
Je, Kang-Hoon
Guillen Quispe, Yanymee N.
Shin, Kyong-Oh
Kim, Hyun Yong
Kim, Kang Hyuk
Arce, Paul H. Gonzales
Lim, Soon Sung
Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling
title Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling
title_full Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling
title_fullStr Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling
title_full_unstemmed Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling
title_short Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling
title_sort separation and identification of antioxidants and aldose reductase inhibitors in lepechinia meyenii (walp.) epling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707451/
https://www.ncbi.nlm.nih.gov/pubmed/34961244
http://dx.doi.org/10.3390/plants10122773
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