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MSC Secretome as a Promising Tool for Neuroprotection and Neuroregeneration in a Model of Intracerebral Hemorrhage

Multipotent mesenchymal stromal cells (MSCs) are considered to be critical contributors to injured tissue repair and regeneration, and MSC-based therapeutic approaches have been applied to many peripheral and central neurologic disorders. It has been demonstrated that the beneficial effects of MSC a...

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Autores principales: Karagyaur, Maxim, Dzhauari, Stalik, Basalova, Nataliya, Aleksandrushkina, Natalia, Sagaradze, Georgy, Danilova, Natalia, Malkov, Pavel, Popov, Vladimir, Skryabina, Mariya, Efimenko, Anastasia, Tkachuk, Vsevolod
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707464/
https://www.ncbi.nlm.nih.gov/pubmed/34959314
http://dx.doi.org/10.3390/pharmaceutics13122031
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author Karagyaur, Maxim
Dzhauari, Stalik
Basalova, Nataliya
Aleksandrushkina, Natalia
Sagaradze, Georgy
Danilova, Natalia
Malkov, Pavel
Popov, Vladimir
Skryabina, Mariya
Efimenko, Anastasia
Tkachuk, Vsevolod
author_facet Karagyaur, Maxim
Dzhauari, Stalik
Basalova, Nataliya
Aleksandrushkina, Natalia
Sagaradze, Georgy
Danilova, Natalia
Malkov, Pavel
Popov, Vladimir
Skryabina, Mariya
Efimenko, Anastasia
Tkachuk, Vsevolod
author_sort Karagyaur, Maxim
collection PubMed
description Multipotent mesenchymal stromal cells (MSCs) are considered to be critical contributors to injured tissue repair and regeneration, and MSC-based therapeutic approaches have been applied to many peripheral and central neurologic disorders. It has been demonstrated that the beneficial effects of MSC are mainly mediated by the components of their secretome. In the current study, we have explored the neuroprotective potential of the MSC secretome in a rat model of intracerebral hemorrhage and shown that a 10-fold concentrated secretome of human MSC and its combination with the brain-derived neurotrophic factor (BDNF) provided a better survival and neurological outcome of rats within 14 days of intracerebral hemorrhage compared to the negative (non-treated) and positive (BDNF) control groups. We found that it was due to the ability of MSC secretome to stimulate neuron survival under conditions of glutamate-induced neurotoxicity. However, the lesion volume did not shrink in these rats, and this also correlated with prominent microglia activation. We hypothesize that this could be caused by the species-specificity of the used MSC secretome and provide evidence to confirm this. Thus, we have found that allogenic rat MSC secretome was more effective than xenogenic human MSC secretome in the rat intracerebral hemorrhage model: it reduced the volume of the lesion and promoted excellent survival and neurological outcome of the treated rats.
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spelling pubmed-87074642021-12-25 MSC Secretome as a Promising Tool for Neuroprotection and Neuroregeneration in a Model of Intracerebral Hemorrhage Karagyaur, Maxim Dzhauari, Stalik Basalova, Nataliya Aleksandrushkina, Natalia Sagaradze, Georgy Danilova, Natalia Malkov, Pavel Popov, Vladimir Skryabina, Mariya Efimenko, Anastasia Tkachuk, Vsevolod Pharmaceutics Article Multipotent mesenchymal stromal cells (MSCs) are considered to be critical contributors to injured tissue repair and regeneration, and MSC-based therapeutic approaches have been applied to many peripheral and central neurologic disorders. It has been demonstrated that the beneficial effects of MSC are mainly mediated by the components of their secretome. In the current study, we have explored the neuroprotective potential of the MSC secretome in a rat model of intracerebral hemorrhage and shown that a 10-fold concentrated secretome of human MSC and its combination with the brain-derived neurotrophic factor (BDNF) provided a better survival and neurological outcome of rats within 14 days of intracerebral hemorrhage compared to the negative (non-treated) and positive (BDNF) control groups. We found that it was due to the ability of MSC secretome to stimulate neuron survival under conditions of glutamate-induced neurotoxicity. However, the lesion volume did not shrink in these rats, and this also correlated with prominent microglia activation. We hypothesize that this could be caused by the species-specificity of the used MSC secretome and provide evidence to confirm this. Thus, we have found that allogenic rat MSC secretome was more effective than xenogenic human MSC secretome in the rat intracerebral hemorrhage model: it reduced the volume of the lesion and promoted excellent survival and neurological outcome of the treated rats. MDPI 2021-11-29 /pmc/articles/PMC8707464/ /pubmed/34959314 http://dx.doi.org/10.3390/pharmaceutics13122031 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Karagyaur, Maxim
Dzhauari, Stalik
Basalova, Nataliya
Aleksandrushkina, Natalia
Sagaradze, Georgy
Danilova, Natalia
Malkov, Pavel
Popov, Vladimir
Skryabina, Mariya
Efimenko, Anastasia
Tkachuk, Vsevolod
MSC Secretome as a Promising Tool for Neuroprotection and Neuroregeneration in a Model of Intracerebral Hemorrhage
title MSC Secretome as a Promising Tool for Neuroprotection and Neuroregeneration in a Model of Intracerebral Hemorrhage
title_full MSC Secretome as a Promising Tool for Neuroprotection and Neuroregeneration in a Model of Intracerebral Hemorrhage
title_fullStr MSC Secretome as a Promising Tool for Neuroprotection and Neuroregeneration in a Model of Intracerebral Hemorrhage
title_full_unstemmed MSC Secretome as a Promising Tool for Neuroprotection and Neuroregeneration in a Model of Intracerebral Hemorrhage
title_short MSC Secretome as a Promising Tool for Neuroprotection and Neuroregeneration in a Model of Intracerebral Hemorrhage
title_sort msc secretome as a promising tool for neuroprotection and neuroregeneration in a model of intracerebral hemorrhage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707464/
https://www.ncbi.nlm.nih.gov/pubmed/34959314
http://dx.doi.org/10.3390/pharmaceutics13122031
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