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A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders

Diagnosis of SARS-CoV-2 infections is mostly based on the nasopharyngeal swabs (NPS). However, this collection is invasive and uncomfortable, especially for children and patients with coagulopathies, whose NPS collection often causes bleeding. Thus, the aim of this study was to evaluate the usefulne...

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Autores principales: Lopes, Josiane Iole França, da Costa Silva, Carlos Alexandre, Cunha, Rodrigo Guimarães, Soares, Alexandra Martins, Lopes, Maria Esther Duarte, da Conceição Neto, Orlando Carlos, Alves, Arthur Daniel Rocha, da Costa Nunes Pimentel Coelho, Wagner Luis, Amorim Filho, Luiz, Amado Leon, Luciane Almeida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707508/
https://www.ncbi.nlm.nih.gov/pubmed/34960630
http://dx.doi.org/10.3390/v13122361
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author Lopes, Josiane Iole França
da Costa Silva, Carlos Alexandre
Cunha, Rodrigo Guimarães
Soares, Alexandra Martins
Lopes, Maria Esther Duarte
da Conceição Neto, Orlando Carlos
Alves, Arthur Daniel Rocha
da Costa Nunes Pimentel Coelho, Wagner Luis
Amorim Filho, Luiz
Amado Leon, Luciane Almeida
author_facet Lopes, Josiane Iole França
da Costa Silva, Carlos Alexandre
Cunha, Rodrigo Guimarães
Soares, Alexandra Martins
Lopes, Maria Esther Duarte
da Conceição Neto, Orlando Carlos
Alves, Arthur Daniel Rocha
da Costa Nunes Pimentel Coelho, Wagner Luis
Amorim Filho, Luiz
Amado Leon, Luciane Almeida
author_sort Lopes, Josiane Iole França
collection PubMed
description Diagnosis of SARS-CoV-2 infections is mostly based on the nasopharyngeal swabs (NPS). However, this collection is invasive and uncomfortable, especially for children and patients with coagulopathies, whose NPS collection often causes bleeding. Thus, the aim of this study was to evaluate the usefulness and accuracy of saliva for the diagnosis of COVID-19 in patients presenting bleeding disorders. Samples of NPS, oropharyngeal swabs (OPS), and saliva were collected simultaneously from 1159 hospitalized patients with hematological diseases and from 524 healthcare workers, both symptomatic and asymptomatic for SARS-CoV-2. All samples were evaluated for SARS-CoV-2 by qRT-PCR. SARS-CoV-2 was detected in NPS, OPS and saliva from 16.9%, 14.4% and 15.6% individuals, respectively. Tests in saliva showed sensitivity, specificity, and overall agreement of 73.3%, 96.9% and 92.7% (=0.74), respectively. Salivary tests had good accuracy (AUC = 0.7) for discriminating negative and positive qRT-PCR for SARS-CoV-2. Higher sensitivity was observed in symptomatic than in non-symptomatic patients, as well as in healthy subjects than in patients with hematological disease, in both OPS and saliva. The mean viral load in NPS was significantly higher than in OPS and in saliva samples (p < 0.001). Saliva is a good diagnostic tool to detect SARS-CoV-2, especially among patients symptomatic for COVID-19, and is a valuable specimen for mass screening of hospitalized patients with hematological diseases, especially for those that with bleeding disorders.
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spelling pubmed-87075082021-12-25 A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders Lopes, Josiane Iole França da Costa Silva, Carlos Alexandre Cunha, Rodrigo Guimarães Soares, Alexandra Martins Lopes, Maria Esther Duarte da Conceição Neto, Orlando Carlos Alves, Arthur Daniel Rocha da Costa Nunes Pimentel Coelho, Wagner Luis Amorim Filho, Luiz Amado Leon, Luciane Almeida Viruses Article Diagnosis of SARS-CoV-2 infections is mostly based on the nasopharyngeal swabs (NPS). However, this collection is invasive and uncomfortable, especially for children and patients with coagulopathies, whose NPS collection often causes bleeding. Thus, the aim of this study was to evaluate the usefulness and accuracy of saliva for the diagnosis of COVID-19 in patients presenting bleeding disorders. Samples of NPS, oropharyngeal swabs (OPS), and saliva were collected simultaneously from 1159 hospitalized patients with hematological diseases and from 524 healthcare workers, both symptomatic and asymptomatic for SARS-CoV-2. All samples were evaluated for SARS-CoV-2 by qRT-PCR. SARS-CoV-2 was detected in NPS, OPS and saliva from 16.9%, 14.4% and 15.6% individuals, respectively. Tests in saliva showed sensitivity, specificity, and overall agreement of 73.3%, 96.9% and 92.7% (=0.74), respectively. Salivary tests had good accuracy (AUC = 0.7) for discriminating negative and positive qRT-PCR for SARS-CoV-2. Higher sensitivity was observed in symptomatic than in non-symptomatic patients, as well as in healthy subjects than in patients with hematological disease, in both OPS and saliva. The mean viral load in NPS was significantly higher than in OPS and in saliva samples (p < 0.001). Saliva is a good diagnostic tool to detect SARS-CoV-2, especially among patients symptomatic for COVID-19, and is a valuable specimen for mass screening of hospitalized patients with hematological diseases, especially for those that with bleeding disorders. MDPI 2021-11-25 /pmc/articles/PMC8707508/ /pubmed/34960630 http://dx.doi.org/10.3390/v13122361 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lopes, Josiane Iole França
da Costa Silva, Carlos Alexandre
Cunha, Rodrigo Guimarães
Soares, Alexandra Martins
Lopes, Maria Esther Duarte
da Conceição Neto, Orlando Carlos
Alves, Arthur Daniel Rocha
da Costa Nunes Pimentel Coelho, Wagner Luis
Amorim Filho, Luiz
Amado Leon, Luciane Almeida
A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders
title A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders
title_full A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders
title_fullStr A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders
title_full_unstemmed A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders
title_short A Large Cohort Study of SARS-CoV-2 Detection in Saliva: A Non-Invasive Alternative Diagnostic Test for Patients with Bleeding Disorders
title_sort large cohort study of sars-cov-2 detection in saliva: a non-invasive alternative diagnostic test for patients with bleeding disorders
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707508/
https://www.ncbi.nlm.nih.gov/pubmed/34960630
http://dx.doi.org/10.3390/v13122361
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