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The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole

During its development, the leaf undergoes profound metabolic changes to ensure, among other things, its growth. The subcellular metabolome of tomato leaves was studied at four stages of leaf development, with a particular emphasis on the composition of the vacuole, a major actor of cell growth. For...

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Autores principales: Destailleur, Alice, Poucet, Théo, Cabasson, Cécile, Alonso, Ana Paula, Cocuron, Jean-Christophe, Larbat, Romain, Vercambre, Gilles, Colombié, Sophie, Petriacq, Pierre, Andrieu, Marie Hélène, Beauvoit, Bertrand, Gibon, Yves, Dieuaide-Noubhani, Martine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707551/
https://www.ncbi.nlm.nih.gov/pubmed/34940606
http://dx.doi.org/10.3390/metabo11120848
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author Destailleur, Alice
Poucet, Théo
Cabasson, Cécile
Alonso, Ana Paula
Cocuron, Jean-Christophe
Larbat, Romain
Vercambre, Gilles
Colombié, Sophie
Petriacq, Pierre
Andrieu, Marie Hélène
Beauvoit, Bertrand
Gibon, Yves
Dieuaide-Noubhani, Martine
author_facet Destailleur, Alice
Poucet, Théo
Cabasson, Cécile
Alonso, Ana Paula
Cocuron, Jean-Christophe
Larbat, Romain
Vercambre, Gilles
Colombié, Sophie
Petriacq, Pierre
Andrieu, Marie Hélène
Beauvoit, Bertrand
Gibon, Yves
Dieuaide-Noubhani, Martine
author_sort Destailleur, Alice
collection PubMed
description During its development, the leaf undergoes profound metabolic changes to ensure, among other things, its growth. The subcellular metabolome of tomato leaves was studied at four stages of leaf development, with a particular emphasis on the composition of the vacuole, a major actor of cell growth. For this, leaves were collected at different positions of the plant, corresponding to different developmental stages. Coupling cytology approaches to non-aqueous cell fractionation allowed to estimate the subcellular concentrations of major compounds in the leaves. The results showed major changes in the composition of the vacuole across leaf development. Thus, sucrose underwent a strong allocation, being mostly located in the vacuole at the beginning of development and in the cytosol at maturity. Furthermore, these analyses revealed that the vacuole, rather rich in secondary metabolites and sugars in the growth phases, accumulated organic acids thereafter. This result suggests that the maintenance of the osmolarity of the vacuole of mature leaves would largely involve inorganic molecules.
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spelling pubmed-87075512021-12-25 The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole Destailleur, Alice Poucet, Théo Cabasson, Cécile Alonso, Ana Paula Cocuron, Jean-Christophe Larbat, Romain Vercambre, Gilles Colombié, Sophie Petriacq, Pierre Andrieu, Marie Hélène Beauvoit, Bertrand Gibon, Yves Dieuaide-Noubhani, Martine Metabolites Article During its development, the leaf undergoes profound metabolic changes to ensure, among other things, its growth. The subcellular metabolome of tomato leaves was studied at four stages of leaf development, with a particular emphasis on the composition of the vacuole, a major actor of cell growth. For this, leaves were collected at different positions of the plant, corresponding to different developmental stages. Coupling cytology approaches to non-aqueous cell fractionation allowed to estimate the subcellular concentrations of major compounds in the leaves. The results showed major changes in the composition of the vacuole across leaf development. Thus, sucrose underwent a strong allocation, being mostly located in the vacuole at the beginning of development and in the cytosol at maturity. Furthermore, these analyses revealed that the vacuole, rather rich in secondary metabolites and sugars in the growth phases, accumulated organic acids thereafter. This result suggests that the maintenance of the osmolarity of the vacuole of mature leaves would largely involve inorganic molecules. MDPI 2021-12-06 /pmc/articles/PMC8707551/ /pubmed/34940606 http://dx.doi.org/10.3390/metabo11120848 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Destailleur, Alice
Poucet, Théo
Cabasson, Cécile
Alonso, Ana Paula
Cocuron, Jean-Christophe
Larbat, Romain
Vercambre, Gilles
Colombié, Sophie
Petriacq, Pierre
Andrieu, Marie Hélène
Beauvoit, Bertrand
Gibon, Yves
Dieuaide-Noubhani, Martine
The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole
title The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole
title_full The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole
title_fullStr The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole
title_full_unstemmed The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole
title_short The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole
title_sort evolution of leaf function during development is reflected in profound changes in the metabolic composition of the vacuole
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707551/
https://www.ncbi.nlm.nih.gov/pubmed/34940606
http://dx.doi.org/10.3390/metabo11120848
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