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Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells
Following a Chlamydia trachomatis infection, the host immune response is characterized by its recognition via Toll-like and Nod-like Receptors, and the subsequent activation of interferon (IFN)-γ-mediated signaling pathways. Recently, the inflammasome-mediated host cell response has emerged to play...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707573/ https://www.ncbi.nlm.nih.gov/pubmed/34947890 http://dx.doi.org/10.3390/life11121359 |
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author | Filardo, Simone Di Pietro, Marisa Frasca, Federica Diaco, Fabiana Scordio, Mirko Antonelli, Guido Scagnolari, Carolina Sessa, Rosa |
author_facet | Filardo, Simone Di Pietro, Marisa Frasca, Federica Diaco, Fabiana Scordio, Mirko Antonelli, Guido Scagnolari, Carolina Sessa, Rosa |
author_sort | Filardo, Simone |
collection | PubMed |
description | Following a Chlamydia trachomatis infection, the host immune response is characterized by its recognition via Toll-like and Nod-like Receptors, and the subsequent activation of interferon (IFN)-γ-mediated signaling pathways. Recently, the inflammasome-mediated host cell response has emerged to play a role in the physiopathology of C. trachomatis infection. Here we investigated, for the first time, the interaction of IFN-γ and inflammasome in an in vitro model of C. trachomatis-infected primary human synovial cells. Chlamydial replication as well as the expression of caspase-1, IL-1β, as well as IL-18 and IL-6, were assayed. Our results demonstrated the inhibitory activity of IFN-γ by interfering with the inflammasome network through the downregulation of caspase-1 mRNA expression. In addition, the ability of C. trachomatis to hinder the inflammasome pathway favoring its intracellular survival within synovial cells, was observed. Overall, our data suggest a potential mechanism of immune evasion by C. trachomatis in synovial cells, that may be contested by IFN-γ. |
format | Online Article Text |
id | pubmed-8707573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87075732021-12-25 Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells Filardo, Simone Di Pietro, Marisa Frasca, Federica Diaco, Fabiana Scordio, Mirko Antonelli, Guido Scagnolari, Carolina Sessa, Rosa Life (Basel) Article Following a Chlamydia trachomatis infection, the host immune response is characterized by its recognition via Toll-like and Nod-like Receptors, and the subsequent activation of interferon (IFN)-γ-mediated signaling pathways. Recently, the inflammasome-mediated host cell response has emerged to play a role in the physiopathology of C. trachomatis infection. Here we investigated, for the first time, the interaction of IFN-γ and inflammasome in an in vitro model of C. trachomatis-infected primary human synovial cells. Chlamydial replication as well as the expression of caspase-1, IL-1β, as well as IL-18 and IL-6, were assayed. Our results demonstrated the inhibitory activity of IFN-γ by interfering with the inflammasome network through the downregulation of caspase-1 mRNA expression. In addition, the ability of C. trachomatis to hinder the inflammasome pathway favoring its intracellular survival within synovial cells, was observed. Overall, our data suggest a potential mechanism of immune evasion by C. trachomatis in synovial cells, that may be contested by IFN-γ. MDPI 2021-12-07 /pmc/articles/PMC8707573/ /pubmed/34947890 http://dx.doi.org/10.3390/life11121359 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Filardo, Simone Di Pietro, Marisa Frasca, Federica Diaco, Fabiana Scordio, Mirko Antonelli, Guido Scagnolari, Carolina Sessa, Rosa Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells |
title | Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells |
title_full | Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells |
title_fullStr | Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells |
title_full_unstemmed | Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells |
title_short | Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells |
title_sort | potential ifnγ modulation of inflammasome pathway in chlamydia trachomatis infected synovial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707573/ https://www.ncbi.nlm.nih.gov/pubmed/34947890 http://dx.doi.org/10.3390/life11121359 |
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