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Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells

Following a Chlamydia trachomatis infection, the host immune response is characterized by its recognition via Toll-like and Nod-like Receptors, and the subsequent activation of interferon (IFN)-γ-mediated signaling pathways. Recently, the inflammasome-mediated host cell response has emerged to play...

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Autores principales: Filardo, Simone, Di Pietro, Marisa, Frasca, Federica, Diaco, Fabiana, Scordio, Mirko, Antonelli, Guido, Scagnolari, Carolina, Sessa, Rosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707573/
https://www.ncbi.nlm.nih.gov/pubmed/34947890
http://dx.doi.org/10.3390/life11121359
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author Filardo, Simone
Di Pietro, Marisa
Frasca, Federica
Diaco, Fabiana
Scordio, Mirko
Antonelli, Guido
Scagnolari, Carolina
Sessa, Rosa
author_facet Filardo, Simone
Di Pietro, Marisa
Frasca, Federica
Diaco, Fabiana
Scordio, Mirko
Antonelli, Guido
Scagnolari, Carolina
Sessa, Rosa
author_sort Filardo, Simone
collection PubMed
description Following a Chlamydia trachomatis infection, the host immune response is characterized by its recognition via Toll-like and Nod-like Receptors, and the subsequent activation of interferon (IFN)-γ-mediated signaling pathways. Recently, the inflammasome-mediated host cell response has emerged to play a role in the physiopathology of C. trachomatis infection. Here we investigated, for the first time, the interaction of IFN-γ and inflammasome in an in vitro model of C. trachomatis-infected primary human synovial cells. Chlamydial replication as well as the expression of caspase-1, IL-1β, as well as IL-18 and IL-6, were assayed. Our results demonstrated the inhibitory activity of IFN-γ by interfering with the inflammasome network through the downregulation of caspase-1 mRNA expression. In addition, the ability of C. trachomatis to hinder the inflammasome pathway favoring its intracellular survival within synovial cells, was observed. Overall, our data suggest a potential mechanism of immune evasion by C. trachomatis in synovial cells, that may be contested by IFN-γ.
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spelling pubmed-87075732021-12-25 Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells Filardo, Simone Di Pietro, Marisa Frasca, Federica Diaco, Fabiana Scordio, Mirko Antonelli, Guido Scagnolari, Carolina Sessa, Rosa Life (Basel) Article Following a Chlamydia trachomatis infection, the host immune response is characterized by its recognition via Toll-like and Nod-like Receptors, and the subsequent activation of interferon (IFN)-γ-mediated signaling pathways. Recently, the inflammasome-mediated host cell response has emerged to play a role in the physiopathology of C. trachomatis infection. Here we investigated, for the first time, the interaction of IFN-γ and inflammasome in an in vitro model of C. trachomatis-infected primary human synovial cells. Chlamydial replication as well as the expression of caspase-1, IL-1β, as well as IL-18 and IL-6, were assayed. Our results demonstrated the inhibitory activity of IFN-γ by interfering with the inflammasome network through the downregulation of caspase-1 mRNA expression. In addition, the ability of C. trachomatis to hinder the inflammasome pathway favoring its intracellular survival within synovial cells, was observed. Overall, our data suggest a potential mechanism of immune evasion by C. trachomatis in synovial cells, that may be contested by IFN-γ. MDPI 2021-12-07 /pmc/articles/PMC8707573/ /pubmed/34947890 http://dx.doi.org/10.3390/life11121359 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Filardo, Simone
Di Pietro, Marisa
Frasca, Federica
Diaco, Fabiana
Scordio, Mirko
Antonelli, Guido
Scagnolari, Carolina
Sessa, Rosa
Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells
title Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells
title_full Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells
title_fullStr Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells
title_full_unstemmed Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells
title_short Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells
title_sort potential ifnγ modulation of inflammasome pathway in chlamydia trachomatis infected synovial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707573/
https://www.ncbi.nlm.nih.gov/pubmed/34947890
http://dx.doi.org/10.3390/life11121359
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