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Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments

During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implement...

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Autores principales: Pavletić, Martina, Mazor, Marija, Lerga, Mate, Mileta, Tatjana, Železnjak, Jelena, Ružić, Tina, Ravlić, Sanda, Palčevski, Dora, Kirinčić, Jelena, Mežnarić, Silvestar, Mišković, Ana, Materljan, Maja, Šustić, Alan, Lisnić, Berislav, Juranić Lisnić, Vanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707628/
https://www.ncbi.nlm.nih.gov/pubmed/34960682
http://dx.doi.org/10.3390/v13122413
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author Pavletić, Martina
Mazor, Marija
Lerga, Mate
Mileta, Tatjana
Železnjak, Jelena
Ružić, Tina
Ravlić, Sanda
Palčevski, Dora
Kirinčić, Jelena
Mežnarić, Silvestar
Mišković, Ana
Materljan, Maja
Šustić, Alan
Lisnić, Berislav
Juranić Lisnić, Vanda
author_facet Pavletić, Martina
Mazor, Marija
Lerga, Mate
Mileta, Tatjana
Železnjak, Jelena
Ružić, Tina
Ravlić, Sanda
Palčevski, Dora
Kirinčić, Jelena
Mežnarić, Silvestar
Mišković, Ana
Materljan, Maja
Šustić, Alan
Lisnić, Berislav
Juranić Lisnić, Vanda
author_sort Pavletić, Martina
collection PubMed
description During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.
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spelling pubmed-87076282021-12-25 Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments Pavletić, Martina Mazor, Marija Lerga, Mate Mileta, Tatjana Železnjak, Jelena Ružić, Tina Ravlić, Sanda Palčevski, Dora Kirinčić, Jelena Mežnarić, Silvestar Mišković, Ana Materljan, Maja Šustić, Alan Lisnić, Berislav Juranić Lisnić, Vanda Viruses Article During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR. MDPI 2021-12-02 /pmc/articles/PMC8707628/ /pubmed/34960682 http://dx.doi.org/10.3390/v13122413 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pavletić, Martina
Mazor, Marija
Lerga, Mate
Mileta, Tatjana
Železnjak, Jelena
Ružić, Tina
Ravlić, Sanda
Palčevski, Dora
Kirinčić, Jelena
Mežnarić, Silvestar
Mišković, Ana
Materljan, Maja
Šustić, Alan
Lisnić, Berislav
Juranić Lisnić, Vanda
Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
title Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
title_full Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
title_fullStr Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
title_full_unstemmed Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
title_short Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
title_sort fast, reliable, and simple point-of-care-like adaptation of rt-qpcr for the detection of sars-cov-2 for use in hospital emergency departments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707628/
https://www.ncbi.nlm.nih.gov/pubmed/34960682
http://dx.doi.org/10.3390/v13122413
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