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Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species
Neofusicoccum parvum belongs to the Botryosphaeriaceae family, which contains endophytes and pathogens of woody plants. In this study, we isolated 11 strains from diseased tissue of Liquidambar styraciflua. Testing with Koch’s postulates—followed by a molecular approach—revealed that N. parvum was t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707630/ https://www.ncbi.nlm.nih.gov/pubmed/34947009 http://dx.doi.org/10.3390/jof7121027 |
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author | Vázquez-Avendaño, Rebeca Rodríguez-Haas, José Benjamín Velázquez-Delgado, Hugo Rosas-Saito, Greta Hanako Hernández-Domínguez, Eric Edmundo Sánchez-Rangel, Diana |
author_facet | Vázquez-Avendaño, Rebeca Rodríguez-Haas, José Benjamín Velázquez-Delgado, Hugo Rosas-Saito, Greta Hanako Hernández-Domínguez, Eric Edmundo Sánchez-Rangel, Diana |
author_sort | Vázquez-Avendaño, Rebeca |
collection | PubMed |
description | Neofusicoccum parvum belongs to the Botryosphaeriaceae family, which contains endophytes and pathogens of woody plants. In this study, we isolated 11 strains from diseased tissue of Liquidambar styraciflua. Testing with Koch’s postulates—followed by a molecular approach—revealed that N. parvum was the most pathogenic strain. We established an in vitro pathosystem (L. styraciflua foliar tissue–N. parvum) in order to characterize the infection process during the first 16 days. New CysRPs were identified for both organisms using public transcriptomic and genomic databases, while mRNA expression of CysRPs was analyzed by RT-qPCR. The results showed that N. parvum caused disease symptoms after 24 h that intensified over time. Through in silico analysis, 5 CysRPs were identified for each organism, revealing that all of the proteins are potentially secreted and novel, including two of N. parvum proteins containing the CFEM domain. Interestingly, the levels of the CysRPs mRNAs change during the interaction. This study reports N. parvum as a pathogen of L. styraciflua for the first time and highlights the potential involvement of CysRPs in both organisms during this interaction. |
format | Online Article Text |
id | pubmed-8707630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87076302021-12-25 Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species Vázquez-Avendaño, Rebeca Rodríguez-Haas, José Benjamín Velázquez-Delgado, Hugo Rosas-Saito, Greta Hanako Hernández-Domínguez, Eric Edmundo Sánchez-Rangel, Diana J Fungi (Basel) Article Neofusicoccum parvum belongs to the Botryosphaeriaceae family, which contains endophytes and pathogens of woody plants. In this study, we isolated 11 strains from diseased tissue of Liquidambar styraciflua. Testing with Koch’s postulates—followed by a molecular approach—revealed that N. parvum was the most pathogenic strain. We established an in vitro pathosystem (L. styraciflua foliar tissue–N. parvum) in order to characterize the infection process during the first 16 days. New CysRPs were identified for both organisms using public transcriptomic and genomic databases, while mRNA expression of CysRPs was analyzed by RT-qPCR. The results showed that N. parvum caused disease symptoms after 24 h that intensified over time. Through in silico analysis, 5 CysRPs were identified for each organism, revealing that all of the proteins are potentially secreted and novel, including two of N. parvum proteins containing the CFEM domain. Interestingly, the levels of the CysRPs mRNAs change during the interaction. This study reports N. parvum as a pathogen of L. styraciflua for the first time and highlights the potential involvement of CysRPs in both organisms during this interaction. MDPI 2021-11-30 /pmc/articles/PMC8707630/ /pubmed/34947009 http://dx.doi.org/10.3390/jof7121027 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vázquez-Avendaño, Rebeca Rodríguez-Haas, José Benjamín Velázquez-Delgado, Hugo Rosas-Saito, Greta Hanako Hernández-Domínguez, Eric Edmundo Sánchez-Rangel, Diana Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species |
title | Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species |
title_full | Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species |
title_fullStr | Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species |
title_full_unstemmed | Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species |
title_short | Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species |
title_sort | insights of the neofusicoccum parvum–liquidambar styraciflua interaction and identification of new cysteine-rich proteins in both species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707630/ https://www.ncbi.nlm.nih.gov/pubmed/34947009 http://dx.doi.org/10.3390/jof7121027 |
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